Using targeted enrichment of nuclear genes to increase phylogenetic resolution in the neotropical rain forest genus Inga (Leguminosae: Mimosoideae)
Evolutionary radiations are prominent and pervasive across many plant lineages in diverse geographical and ecological settings; in neotropical rainforests there is growing evidence suggesting that a significant fraction of species richness is the result of recent radiations. Understanding the evolu...
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doaj-1d59eb9b27d14a60902186d91408c0cb2020-11-24T23:16:58ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2015-09-01610.3389/fpls.2015.00710153142Using targeted enrichment of nuclear genes to increase phylogenetic resolution in the neotropical rain forest genus Inga (Leguminosae: Mimosoideae)James A Nicholls0R. Toby Pennington1Erik Jozef, Mathieu Koenen2Colin Edward Hughes3Jack eHearn4Lynsey eBunnefeld5Kyle Graham Dexter6Graham Nicholas Stone7Catherine Anne Kidner8Catherine Anne Kidner9University of EdinburghRoyal Botanic Garden EdinburghUniversity of ZurichUniversity of ZurichUniversity of EdinburghUniversity of EdinburghUniversity of EdinburghUniversity of EdinburghUniversity of EdinburghRoyal Botanic Garden EdinburghEvolutionary radiations are prominent and pervasive across many plant lineages in diverse geographical and ecological settings; in neotropical rainforests there is growing evidence suggesting that a significant fraction of species richness is the result of recent radiations. Understanding the evolutionary trajectories and mechanisms underlying these radiations demands much greater phylogenetic resolution than is currently available for these groups. The neotropical tree genus Inga (Leguminosae) is a good example, with ~300 extant species and a crown age of 2-10 MY, yet over 6kb of plastid and nuclear DNA sequence data gives only poor phylogenetic resolution among species. Here we explore the use of larger-scale nuclear gene data obtained though targeted enrichment to increase phylogenetic resolution within Inga. Transcriptome data from three Inga species were used to select 264 nuclear loci for targeted enrichment and sequencing. Following quality control to remove probable paralogs from these sequence data, the final dataset comprised 259,313 bases from 194 loci for 24 accessions representing 22 Inga species and an outgroup (Zygia). Bayesian phylogenies reconstructed using either all loci concatenated or a subset of 60 loci in a gene-tree/species-tree approach yielded highly resolved phylogenies. We used coalescent approaches to show that the same targeted enrichment data also have significant power to discriminate among alternative within-species population histories in the widespread species I. umbellifera. In either application, targeted enrichment simplifies the informatics challenge of identifying orthologous loci associated with de novo genome sequencing. We conclude that targeted enrichment provides the large volumes of phylogenetically-informative sequence data required to resolve relationships within recent plant species radiations, both at the species level and for within-species phylogeographic studies.http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00710/fullRadiationNext-generation sequencingphylogenomicsPopulation GenomicsInGaHybrid Capture |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
James A Nicholls R. Toby Pennington Erik Jozef, Mathieu Koenen Colin Edward Hughes Jack eHearn Lynsey eBunnefeld Kyle Graham Dexter Graham Nicholas Stone Catherine Anne Kidner Catherine Anne Kidner |
spellingShingle |
James A Nicholls R. Toby Pennington Erik Jozef, Mathieu Koenen Colin Edward Hughes Jack eHearn Lynsey eBunnefeld Kyle Graham Dexter Graham Nicholas Stone Catherine Anne Kidner Catherine Anne Kidner Using targeted enrichment of nuclear genes to increase phylogenetic resolution in the neotropical rain forest genus Inga (Leguminosae: Mimosoideae) Frontiers in Plant Science Radiation Next-generation sequencing phylogenomics Population Genomics InGa Hybrid Capture |
author_facet |
James A Nicholls R. Toby Pennington Erik Jozef, Mathieu Koenen Colin Edward Hughes Jack eHearn Lynsey eBunnefeld Kyle Graham Dexter Graham Nicholas Stone Catherine Anne Kidner Catherine Anne Kidner |
author_sort |
James A Nicholls |
title |
Using targeted enrichment of nuclear genes to increase phylogenetic resolution in the neotropical rain forest genus Inga (Leguminosae: Mimosoideae) |
title_short |
Using targeted enrichment of nuclear genes to increase phylogenetic resolution in the neotropical rain forest genus Inga (Leguminosae: Mimosoideae) |
title_full |
Using targeted enrichment of nuclear genes to increase phylogenetic resolution in the neotropical rain forest genus Inga (Leguminosae: Mimosoideae) |
title_fullStr |
Using targeted enrichment of nuclear genes to increase phylogenetic resolution in the neotropical rain forest genus Inga (Leguminosae: Mimosoideae) |
title_full_unstemmed |
Using targeted enrichment of nuclear genes to increase phylogenetic resolution in the neotropical rain forest genus Inga (Leguminosae: Mimosoideae) |
title_sort |
using targeted enrichment of nuclear genes to increase phylogenetic resolution in the neotropical rain forest genus inga (leguminosae: mimosoideae) |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2015-09-01 |
description |
Evolutionary radiations are prominent and pervasive across many plant lineages in diverse geographical and ecological settings; in neotropical rainforests there is growing evidence suggesting that a significant fraction of species richness is the result of recent radiations. Understanding the evolutionary trajectories and mechanisms underlying these radiations demands much greater phylogenetic resolution than is currently available for these groups. The neotropical tree genus Inga (Leguminosae) is a good example, with ~300 extant species and a crown age of 2-10 MY, yet over 6kb of plastid and nuclear DNA sequence data gives only poor phylogenetic resolution among species. Here we explore the use of larger-scale nuclear gene data obtained though targeted enrichment to increase phylogenetic resolution within Inga. Transcriptome data from three Inga species were used to select 264 nuclear loci for targeted enrichment and sequencing. Following quality control to remove probable paralogs from these sequence data, the final dataset comprised 259,313 bases from 194 loci for 24 accessions representing 22 Inga species and an outgroup (Zygia). Bayesian phylogenies reconstructed using either all loci concatenated or a subset of 60 loci in a gene-tree/species-tree approach yielded highly resolved phylogenies. We used coalescent approaches to show that the same targeted enrichment data also have significant power to discriminate among alternative within-species population histories in the widespread species I. umbellifera. In either application, targeted enrichment simplifies the informatics challenge of identifying orthologous loci associated with de novo genome sequencing. We conclude that targeted enrichment provides the large volumes of phylogenetically-informative sequence data required to resolve relationships within recent plant species radiations, both at the species level and for within-species phylogeographic studies. |
topic |
Radiation Next-generation sequencing phylogenomics Population Genomics InGa Hybrid Capture |
url |
http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00710/full |
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