Effects of Cu, Zn and Pb Combined Pollution on Soil Hydrolase Activities

• Main Authors: FENG Dan, WANG Jin-sheng, TENG Yan-guo
• Format: Article
• Language: zho
• Published: Agro-Environmental Protection Institute, Ministry of Agriculture 2015-08-01
• Series: Journal of Agricultural Resources and Environment
• Subjects: heavy metals; orthogonal experiment; invertase; urease; alkaline phosphatase
• Online Access: http://www.aed.org.cn/nyzyyhjxb/html/2015/4/20150414.htm

To study the relations between soil enzyme activities and heavy metal pollution, the combined effects of Cu, Zn and Pb on the three hydrolase activities, including invertase(IN), urease(U)and alkaline phosphatase(ALP)were investigated via an orthogonal experiment. Results showed as the following: When the concentration of Cu was 400 mg·kg-1, the U and ALP activities were decreased 51% and 44%, separately; When Zn was at 500 mg·kg-1, IN and ALP activities were only decreased 3% and 9%, while U activity was increased; When Pb was at 500 mg·kg-1, IN and U activities were increased, while ALP activity was decreased 13%. As a whole, Cu was considered as the most remarkable influence factor for IN, U and ALP activity regardless of interactions among the heavy metals, Zn came second, and Pb mainly showed activation. Considering interactions, Cu×Zn could significantly influence U activity(P<0.05), effects of Cu×Pb and Cu×Zn on ALP activity were remarkable(95% confidence interval). The response of ALP activity was more sensitive than the other two enzymes. Soil ALP activity might be a sensitive tool for assessing the pollution degree of Cu.