Enzyme-Polymers Conjugated to Quantum-Dots for Sensing Applications
In the present research, the concept of developing a novel system based on polymer-enzyme macromolecules was tested by coupling carboxylic acid functionalized poly(vinyl alcohol) (PVA-COOH) to glucose oxidase (GOx) followed by the bioconjugation with CdS quantum-dots (QD). The resulting organic-inor...
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doaj-1d655e01b9494e4c9946cb946184b31e2020-11-24T21:55:48ZengMDPI AGSensors1424-82202011-10-0111109951997210.3390/s111009951Enzyme-Polymers Conjugated to Quantum-Dots for Sensing ApplicationsAlexandra MansurJuan GonzálezHerman MansurIn the present research, the concept of developing a novel system based on polymer-enzyme macromolecules was tested by coupling carboxylic acid functionalized poly(vinyl alcohol) (PVA-COOH) to glucose oxidase (GOx) followed by the bioconjugation with CdS quantum-dots (QD). The resulting organic-inorganic nanohybrids were characterized by UV-visible spectroscopy, infrared spectroscopy, Photoluminescence spectroscopy (PL) and transmission electron microscopy (TEM). The spectroscopy results have clearly shown that the polymer-enzyme macromolecules (PVA-COOH/GOx) were synthesized by the proposed zero-length linker route. Moreover, they have performed as successful capping agents for the nucleation and constrained growth of CdS quantum-dots via aqueous colloidal chemistry. The TEM images associated with the optical absorption results have indicated the formation of CdS nanocrystals with estimated diameters of about 3.0 nm. The “blue-shift” in the visible absorption spectra and the PL values have provided strong evidence that the fluorescent CdS nanoparticles were produced in the quantum-size confinement regime. Finally, the hybrid system was biochemically assayed by injecting the glucose substrate and detecting the formation of peroxide with the enzyme horseradish peroxidase (HRP). Thus, the polymer-enzyme-QD hybrid has behaved as a nanostructured sensor for glucose detecting.http://www.mdpi.com/1424-8220/11/10/9951/hybridsquantum dotsfunctionalized polymerenzyme sensorcolloidsnanossensor |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alexandra Mansur Juan González Herman Mansur |
spellingShingle |
Alexandra Mansur Juan González Herman Mansur Enzyme-Polymers Conjugated to Quantum-Dots for Sensing Applications Sensors hybrids quantum dots functionalized polymer enzyme sensor colloids nanossensor |
author_facet |
Alexandra Mansur Juan González Herman Mansur |
author_sort |
Alexandra Mansur |
title |
Enzyme-Polymers Conjugated to Quantum-Dots for Sensing Applications |
title_short |
Enzyme-Polymers Conjugated to Quantum-Dots for Sensing Applications |
title_full |
Enzyme-Polymers Conjugated to Quantum-Dots for Sensing Applications |
title_fullStr |
Enzyme-Polymers Conjugated to Quantum-Dots for Sensing Applications |
title_full_unstemmed |
Enzyme-Polymers Conjugated to Quantum-Dots for Sensing Applications |
title_sort |
enzyme-polymers conjugated to quantum-dots for sensing applications |
publisher |
MDPI AG |
series |
Sensors |
issn |
1424-8220 |
publishDate |
2011-10-01 |
description |
In the present research, the concept of developing a novel system based on polymer-enzyme macromolecules was tested by coupling carboxylic acid functionalized poly(vinyl alcohol) (PVA-COOH) to glucose oxidase (GOx) followed by the bioconjugation with CdS quantum-dots (QD). The resulting organic-inorganic nanohybrids were characterized by UV-visible spectroscopy, infrared spectroscopy, Photoluminescence spectroscopy (PL) and transmission electron microscopy (TEM). The spectroscopy results have clearly shown that the polymer-enzyme macromolecules (PVA-COOH/GOx) were synthesized by the proposed zero-length linker route. Moreover, they have performed as successful capping agents for the nucleation and constrained growth of CdS quantum-dots via aqueous colloidal chemistry. The TEM images associated with the optical absorption results have indicated the formation of CdS nanocrystals with estimated diameters of about 3.0 nm. The “blue-shift” in the visible absorption spectra and the PL values have provided strong evidence that the fluorescent CdS nanoparticles were produced in the quantum-size confinement regime. Finally, the hybrid system was biochemically assayed by injecting the glucose substrate and detecting the formation of peroxide with the enzyme horseradish peroxidase (HRP). Thus, the polymer-enzyme-QD hybrid has behaved as a nanostructured sensor for glucose detecting. |
topic |
hybrids quantum dots functionalized polymer enzyme sensor colloids nanossensor |
url |
http://www.mdpi.com/1424-8220/11/10/9951/ |
work_keys_str_mv |
AT alexandramansur enzymepolymersconjugatedtoquantumdotsforsensingapplications AT juangonzalez enzymepolymersconjugatedtoquantumdotsforsensingapplications AT hermanmansur enzymepolymersconjugatedtoquantumdotsforsensingapplications |
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