Low Molecular Weight Chitosan-Insulin Complexes Solubilized in a Mixture of Self-Assembled Labrosol and Plurol Oleaque and Their Glucose Reduction Activity in Rats
Oral insulin delivery that better mimics physiological pathways is a necessity as it ensures patient comfort and compliance. A system which is based on a vehicle of nano order where positively charged chitosan interacts with negatively charged insulin and forms a polyelectrolyte complex (PEC) solubi...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2018-01-01
|
Series: | Marine Drugs |
Subjects: | |
Online Access: | http://www.mdpi.com/1660-3397/16/1/32 |
id |
doaj-1d6b8b43874a4d80b1d0205229e0fbaf |
---|---|
record_format |
Article |
spelling |
doaj-1d6b8b43874a4d80b1d0205229e0fbaf2020-11-24T21:15:39ZengMDPI AGMarine Drugs1660-33972018-01-011613210.3390/md16010032md16010032Low Molecular Weight Chitosan-Insulin Complexes Solubilized in a Mixture of Self-Assembled Labrosol and Plurol Oleaque and Their Glucose Reduction Activity in RatsAmani M. Elsayed0Aseel H. Khaled1Mayyas M. Al Remawi2Nidal A. Qinna3Hussam Abu Farsakh4Adnan A. Badwan5Department of Pharmaceutics, College of Pharmacy, Taif University, Taif 26571, Saudi ArabiaThe Jordanian Pharmaceutical Manufacturing Co., Naor 11710, JordanFaculty of Pharmacy and Medical Sciences, University of Petra, Amman 11196, JordanFaculty of Pharmacy and Medical Sciences, University of Petra, Amman 11196, JordanDepartment of Pathology, First Medical Laboratory, Amman, JordanThe Jordanian Pharmaceutical Manufacturing Co., Naor 11710, JordanOral insulin delivery that better mimics physiological pathways is a necessity as it ensures patient comfort and compliance. A system which is based on a vehicle of nano order where positively charged chitosan interacts with negatively charged insulin and forms a polyelectrolyte complex (PEC) solubilizate, which is then solubilized into an oily phase of oleic acid, labrasol, and plurol oleaque-protects insulin against enzymatic gastrointestinal reduction. The use of an anionic fatty acid in the oily phase, such as oleic acid, is thought to allow an interaction with cationic chitosan, hence reducing particle size. Formulations were assessed based on their hypoglycaemic capacities in diabetic rats as compared to conventional subcutaneous dosage forms. 50 IU/kg oral insulin strength could only induce blood glucose reduction equivalent to that of 5 IU/kg (1 International unit = 0.0347 mg of human insulin). Parameters that influence the pharmacological availability were evaluated. A preliminary investigation of the mechanism of absorption suggests the involvement of the lymphatic route.http://www.mdpi.com/1660-3397/16/1/32chitosaninsulinoralsolubilization |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Amani M. Elsayed Aseel H. Khaled Mayyas M. Al Remawi Nidal A. Qinna Hussam Abu Farsakh Adnan A. Badwan |
spellingShingle |
Amani M. Elsayed Aseel H. Khaled Mayyas M. Al Remawi Nidal A. Qinna Hussam Abu Farsakh Adnan A. Badwan Low Molecular Weight Chitosan-Insulin Complexes Solubilized in a Mixture of Self-Assembled Labrosol and Plurol Oleaque and Their Glucose Reduction Activity in Rats Marine Drugs chitosan insulin oral solubilization |
author_facet |
Amani M. Elsayed Aseel H. Khaled Mayyas M. Al Remawi Nidal A. Qinna Hussam Abu Farsakh Adnan A. Badwan |
author_sort |
Amani M. Elsayed |
title |
Low Molecular Weight Chitosan-Insulin Complexes Solubilized in a Mixture of Self-Assembled Labrosol and Plurol Oleaque and Their Glucose Reduction Activity in Rats |
title_short |
Low Molecular Weight Chitosan-Insulin Complexes Solubilized in a Mixture of Self-Assembled Labrosol and Plurol Oleaque and Their Glucose Reduction Activity in Rats |
title_full |
Low Molecular Weight Chitosan-Insulin Complexes Solubilized in a Mixture of Self-Assembled Labrosol and Plurol Oleaque and Their Glucose Reduction Activity in Rats |
title_fullStr |
Low Molecular Weight Chitosan-Insulin Complexes Solubilized in a Mixture of Self-Assembled Labrosol and Plurol Oleaque and Their Glucose Reduction Activity in Rats |
title_full_unstemmed |
Low Molecular Weight Chitosan-Insulin Complexes Solubilized in a Mixture of Self-Assembled Labrosol and Plurol Oleaque and Their Glucose Reduction Activity in Rats |
title_sort |
low molecular weight chitosan-insulin complexes solubilized in a mixture of self-assembled labrosol and plurol oleaque and their glucose reduction activity in rats |
publisher |
MDPI AG |
series |
Marine Drugs |
issn |
1660-3397 |
publishDate |
2018-01-01 |
description |
Oral insulin delivery that better mimics physiological pathways is a necessity as it ensures patient comfort and compliance. A system which is based on a vehicle of nano order where positively charged chitosan interacts with negatively charged insulin and forms a polyelectrolyte complex (PEC) solubilizate, which is then solubilized into an oily phase of oleic acid, labrasol, and plurol oleaque-protects insulin against enzymatic gastrointestinal reduction. The use of an anionic fatty acid in the oily phase, such as oleic acid, is thought to allow an interaction with cationic chitosan, hence reducing particle size. Formulations were assessed based on their hypoglycaemic capacities in diabetic rats as compared to conventional subcutaneous dosage forms. 50 IU/kg oral insulin strength could only induce blood glucose reduction equivalent to that of 5 IU/kg (1 International unit = 0.0347 mg of human insulin). Parameters that influence the pharmacological availability were evaluated. A preliminary investigation of the mechanism of absorption suggests the involvement of the lymphatic route. |
topic |
chitosan insulin oral solubilization |
url |
http://www.mdpi.com/1660-3397/16/1/32 |
work_keys_str_mv |
AT amanimelsayed lowmolecularweightchitosaninsulincomplexessolubilizedinamixtureofselfassembledlabrosolandplurololeaqueandtheirglucosereductionactivityinrats AT aseelhkhaled lowmolecularweightchitosaninsulincomplexessolubilizedinamixtureofselfassembledlabrosolandplurololeaqueandtheirglucosereductionactivityinrats AT mayyasmalremawi lowmolecularweightchitosaninsulincomplexessolubilizedinamixtureofselfassembledlabrosolandplurololeaqueandtheirglucosereductionactivityinrats AT nidalaqinna lowmolecularweightchitosaninsulincomplexessolubilizedinamixtureofselfassembledlabrosolandplurololeaqueandtheirglucosereductionactivityinrats AT hussamabufarsakh lowmolecularweightchitosaninsulincomplexessolubilizedinamixtureofselfassembledlabrosolandplurololeaqueandtheirglucosereductionactivityinrats AT adnanabadwan lowmolecularweightchitosaninsulincomplexessolubilizedinamixtureofselfassembledlabrosolandplurololeaqueandtheirglucosereductionactivityinrats |
_version_ |
1716744475552776192 |