Perimicrovillar membrane assembly: the fate of phospholipids synthesised by the midgut of Rhodnius prolixus
In this study, we describe the fate of fatty acids that are incorporated from the lumen by the posterior midgut epithelium of Rhodnius prolixus and the biosynthesis of lipids. We also demonstrate that neutral lipids (NL) are transferred to the haemolymphatic lipophorin (Lp) and that phospholipids re...
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Instituto Oswaldo Cruz, Ministério da Saúde
2013-06-01
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doaj-1d72c6d639214a1e9b20bd571775ee0f2020-11-24T21:57:51ZengInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz.1678-80602013-06-01108449450010.1590/S0074-0276108042013016S0074-02762013000400494Perimicrovillar membrane assembly: the fate of phospholipids synthesised by the midgut of Rhodnius prolixusPaula Rego Bittencourt-CunhaLivia Silva-CardosoGiselle Almeida de OliveiraJose Roberto da SilvaAlan Barbosa da SilveiraGeorge Eduardo Gabriel KluckMichele Souza-LimaKatia Calp GondimMarilvia Dansa-PetretskyCarlos Peres SilvaHatisaburo MasudaMario Alberto Cardoso da Silva NetoGeorgia Correa AtellaIn this study, we describe the fate of fatty acids that are incorporated from the lumen by the posterior midgut epithelium of Rhodnius prolixus and the biosynthesis of lipids. We also demonstrate that neutral lipids (NL) are transferred to the haemolymphatic lipophorin (Lp) and that phospholipids remain in the tissue in which they are organised into perimicrovillar membranes (PMMs). 3H-palmitic acid added at the luminal side of isolated midguts of R. prolixus females was readily absorbed and was used to synthesise phospholipids (80%) and NL (20%). The highest incorporation of 3H-palmitic acid was on the first day after a blood meal. The amounts of diacylglycerol (DG) and triacylglycerol synthesised by the tissue decreased in the presence of Lp in the incubation medium. The metabolic fates of 3H-lipids synthesised by the posterior midgut were followed and it was observed that DG was the major lipid released to Lp particles. However, the majority of phospholipids were not transferred to Lp, but remained in the tissue. The phospholipids that were synthesised and accumulated in the posterior midgut were found to be associated with Rhodnius luminal contents as structural components of PMMs.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000400494&lng=en&tlng=enlipid metabolismlipoproteinlipid transportphospholipidsmidgut |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Paula Rego Bittencourt-Cunha Livia Silva-Cardoso Giselle Almeida de Oliveira Jose Roberto da Silva Alan Barbosa da Silveira George Eduardo Gabriel Kluck Michele Souza-Lima Katia Calp Gondim Marilvia Dansa-Petretsky Carlos Peres Silva Hatisaburo Masuda Mario Alberto Cardoso da Silva Neto Georgia Correa Atella |
spellingShingle |
Paula Rego Bittencourt-Cunha Livia Silva-Cardoso Giselle Almeida de Oliveira Jose Roberto da Silva Alan Barbosa da Silveira George Eduardo Gabriel Kluck Michele Souza-Lima Katia Calp Gondim Marilvia Dansa-Petretsky Carlos Peres Silva Hatisaburo Masuda Mario Alberto Cardoso da Silva Neto Georgia Correa Atella Perimicrovillar membrane assembly: the fate of phospholipids synthesised by the midgut of Rhodnius prolixus Memórias do Instituto Oswaldo Cruz. lipid metabolism lipoprotein lipid transport phospholipids midgut |
author_facet |
Paula Rego Bittencourt-Cunha Livia Silva-Cardoso Giselle Almeida de Oliveira Jose Roberto da Silva Alan Barbosa da Silveira George Eduardo Gabriel Kluck Michele Souza-Lima Katia Calp Gondim Marilvia Dansa-Petretsky Carlos Peres Silva Hatisaburo Masuda Mario Alberto Cardoso da Silva Neto Georgia Correa Atella |
author_sort |
Paula Rego Bittencourt-Cunha |
title |
Perimicrovillar membrane assembly: the fate of phospholipids synthesised by the midgut of Rhodnius prolixus |
title_short |
Perimicrovillar membrane assembly: the fate of phospholipids synthesised by the midgut of Rhodnius prolixus |
title_full |
Perimicrovillar membrane assembly: the fate of phospholipids synthesised by the midgut of Rhodnius prolixus |
title_fullStr |
Perimicrovillar membrane assembly: the fate of phospholipids synthesised by the midgut of Rhodnius prolixus |
title_full_unstemmed |
Perimicrovillar membrane assembly: the fate of phospholipids synthesised by the midgut of Rhodnius prolixus |
title_sort |
perimicrovillar membrane assembly: the fate of phospholipids synthesised by the midgut of rhodnius prolixus |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
series |
Memórias do Instituto Oswaldo Cruz. |
issn |
1678-8060 |
publishDate |
2013-06-01 |
description |
In this study, we describe the fate of fatty acids that are incorporated from the lumen by the posterior midgut epithelium of Rhodnius prolixus and the biosynthesis of lipids. We also demonstrate that neutral lipids (NL) are transferred to the haemolymphatic lipophorin (Lp) and that phospholipids remain in the tissue in which they are organised into perimicrovillar membranes (PMMs). 3H-palmitic acid added at the luminal side of isolated midguts of R. prolixus females was readily absorbed and was used to synthesise phospholipids (80%) and NL (20%). The highest incorporation of 3H-palmitic acid was on the first day after a blood meal. The amounts of diacylglycerol (DG) and triacylglycerol synthesised by the tissue decreased in the presence of Lp in the incubation medium. The metabolic fates of 3H-lipids synthesised by the posterior midgut were followed and it was observed that DG was the major lipid released to Lp particles. However, the majority of phospholipids were not transferred to Lp, but remained in the tissue. The phospholipids that were synthesised and accumulated in the posterior midgut were found to be associated with Rhodnius luminal contents as structural components of PMMs. |
topic |
lipid metabolism lipoprotein lipid transport phospholipids midgut |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000400494&lng=en&tlng=en |
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