Multilocus sequence typing of clinical Borreliella afzelii strains: population structure and differential ability to disseminate in humans
Abstract Background Lyme borreliosis in humans results in a range of clinical manifestations, thought to be partly due to differences in the pathogenicity of the infecting strain. This study compared European human clinical strains of Borreliella afzelii (previously named Borrelia afzelii) using mul...
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doaj-1d9243c03e8b4296a41e5df3266f53152020-11-25T00:28:17ZengBMCParasites & Vectors1756-33052018-06-0111111310.1186/s13071-018-2938-xMultilocus sequence typing of clinical Borreliella afzelii strains: population structure and differential ability to disseminate in humansFloriane Gallais0Sylvie J. De Martino1Erik A. Sauleau2Yves Hansmann3Dan Lipsker4Cédric Lenormand5Emilie Talagrand-Reboul6Pierre H. Boyer7Nathalie Boulanger8Benoît Jaulhac9Frédéric Schramm10EA7290 Early Bacterial Virulence: Lyme borreliosis Group, FMTS, University of StrasbourgEA7290 Early Bacterial Virulence: Lyme borreliosis Group, FMTS, University of StrasbourgPublic Health, Methods in Clinical Research Team, University Hospital StrasbourgEA7290 Early Bacterial Virulence: Lyme borreliosis Group, FMTS, University of StrasbourgGroupe d’Étude de la Borréliose de Lyme (GEBLY)EA7290 Early Bacterial Virulence: Lyme borreliosis Group, FMTS, University of StrasbourgEA7290 Early Bacterial Virulence: Lyme borreliosis Group, FMTS, University of StrasbourgEA7290 Early Bacterial Virulence: Lyme borreliosis Group, FMTS, University of StrasbourgEA7290 Early Bacterial Virulence: Lyme borreliosis Group, FMTS, University of StrasbourgEA7290 Early Bacterial Virulence: Lyme borreliosis Group, FMTS, University of StrasbourgEA7290 Early Bacterial Virulence: Lyme borreliosis Group, FMTS, University of StrasbourgAbstract Background Lyme borreliosis in humans results in a range of clinical manifestations, thought to be partly due to differences in the pathogenicity of the infecting strain. This study compared European human clinical strains of Borreliella afzelii (previously named Borrelia afzelii) using multilocus sequence typing (MLST) to determine their spatial distribution across Europe and to establish whether there are associations between B. afzelii genotypes and specific clinical manifestations of Lyme borreliosis. For this purpose, typing was performed on 63 strains, and data on a further 245 strains were accessed from the literature. Results All 308 strains were categorized into 149 sequence types (STs), 27 of which are described here for the first time. Phylogenetic and goeBURST analyses showed short evolutionary distances between strains. Although the main STs differed among the countries with the largest number of strains of interest (Germany, the Netherlands, France and Slovenia), the B. afzelii clinical strains were less genetically structured than those previously observed in the European tick population. Two STs were found significantly more frequently in strains associated with clinical manifestations involving erythema migrans, whereas another ST was found significantly more frequently in strains associated with disseminated manifestations, especially neuroborreliosis. Conclusions The MLST profiles showed low genetic differentiation between B. afzelii strains isolated from patients with Lyme borreliosis in Europe. Also, clinical data analysis suggests the existence of lineages with differential dissemination properties in humans.http://link.springer.com/article/10.1186/s13071-018-2938-xBorreliaBorreliellaBorreliella afzeliiMultilocus sequence typingLyme borreliosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Floriane Gallais Sylvie J. De Martino Erik A. Sauleau Yves Hansmann Dan Lipsker Cédric Lenormand Emilie Talagrand-Reboul Pierre H. Boyer Nathalie Boulanger Benoît Jaulhac Frédéric Schramm |
spellingShingle |
Floriane Gallais Sylvie J. De Martino Erik A. Sauleau Yves Hansmann Dan Lipsker Cédric Lenormand Emilie Talagrand-Reboul Pierre H. Boyer Nathalie Boulanger Benoît Jaulhac Frédéric Schramm Multilocus sequence typing of clinical Borreliella afzelii strains: population structure and differential ability to disseminate in humans Parasites & Vectors Borrelia Borreliella Borreliella afzelii Multilocus sequence typing Lyme borreliosis |
author_facet |
Floriane Gallais Sylvie J. De Martino Erik A. Sauleau Yves Hansmann Dan Lipsker Cédric Lenormand Emilie Talagrand-Reboul Pierre H. Boyer Nathalie Boulanger Benoît Jaulhac Frédéric Schramm |
author_sort |
Floriane Gallais |
title |
Multilocus sequence typing of clinical Borreliella afzelii strains: population structure and differential ability to disseminate in humans |
title_short |
Multilocus sequence typing of clinical Borreliella afzelii strains: population structure and differential ability to disseminate in humans |
title_full |
Multilocus sequence typing of clinical Borreliella afzelii strains: population structure and differential ability to disseminate in humans |
title_fullStr |
Multilocus sequence typing of clinical Borreliella afzelii strains: population structure and differential ability to disseminate in humans |
title_full_unstemmed |
Multilocus sequence typing of clinical Borreliella afzelii strains: population structure and differential ability to disseminate in humans |
title_sort |
multilocus sequence typing of clinical borreliella afzelii strains: population structure and differential ability to disseminate in humans |
publisher |
BMC |
series |
Parasites & Vectors |
issn |
1756-3305 |
publishDate |
2018-06-01 |
description |
Abstract Background Lyme borreliosis in humans results in a range of clinical manifestations, thought to be partly due to differences in the pathogenicity of the infecting strain. This study compared European human clinical strains of Borreliella afzelii (previously named Borrelia afzelii) using multilocus sequence typing (MLST) to determine their spatial distribution across Europe and to establish whether there are associations between B. afzelii genotypes and specific clinical manifestations of Lyme borreliosis. For this purpose, typing was performed on 63 strains, and data on a further 245 strains were accessed from the literature. Results All 308 strains were categorized into 149 sequence types (STs), 27 of which are described here for the first time. Phylogenetic and goeBURST analyses showed short evolutionary distances between strains. Although the main STs differed among the countries with the largest number of strains of interest (Germany, the Netherlands, France and Slovenia), the B. afzelii clinical strains were less genetically structured than those previously observed in the European tick population. Two STs were found significantly more frequently in strains associated with clinical manifestations involving erythema migrans, whereas another ST was found significantly more frequently in strains associated with disseminated manifestations, especially neuroborreliosis. Conclusions The MLST profiles showed low genetic differentiation between B. afzelii strains isolated from patients with Lyme borreliosis in Europe. Also, clinical data analysis suggests the existence of lineages with differential dissemination properties in humans. |
topic |
Borrelia Borreliella Borreliella afzelii Multilocus sequence typing Lyme borreliosis |
url |
http://link.springer.com/article/10.1186/s13071-018-2938-x |
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