Transcriptome and proteome analysis of Salmonella enterica serovar Typhimurium systemic infection of wild type and immune-deficient mice.
Salmonella enterica are a threat to public health. Current vaccines are not fully effective. The ability to grow in infected tissues within phagocytes is required for S. enterica virulence in systemic disease. As the infection progresses the bacteria are exposed to a complex host immune response. Co...
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doaj-1d9cfdd9b41d4e28b88fa683244078292020-11-25T01:45:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01128e018136510.1371/journal.pone.0181365Transcriptome and proteome analysis of Salmonella enterica serovar Typhimurium systemic infection of wild type and immune-deficient mice.Olusegun OshotaMax ConwayMaria FookesFernanda SchreiberRoy R ChaudhuriLu YuFiona J E MorganSimon ClareJyoti ChoudharyNicholas R ThomsonPietro LioDuncan J MaskellPietro MastroeniAndrew J GrantSalmonella enterica are a threat to public health. Current vaccines are not fully effective. The ability to grow in infected tissues within phagocytes is required for S. enterica virulence in systemic disease. As the infection progresses the bacteria are exposed to a complex host immune response. Consequently, in order to continue growing in the tissues, S. enterica requires the coordinated regulation of fitness genes. Bacterial gene regulation has so far been investigated largely using exposure to artificial environmental conditions or to in vitro cultured cells, and little information is available on how S. enterica adapts in vivo to sustain cell division and survival. We have studied the transcriptome, proteome and metabolic flux of Salmonella, and the transcriptome of the host during infection of wild type C57BL/6 and immune-deficient gp91-/-phox mice. Our analyses advance the understanding of how S. enterica and the host behaves during infection to a more sophisticated level than has previously been reported.http://europepmc.org/articles/PMC5552096?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Olusegun Oshota Max Conway Maria Fookes Fernanda Schreiber Roy R Chaudhuri Lu Yu Fiona J E Morgan Simon Clare Jyoti Choudhary Nicholas R Thomson Pietro Lio Duncan J Maskell Pietro Mastroeni Andrew J Grant |
spellingShingle |
Olusegun Oshota Max Conway Maria Fookes Fernanda Schreiber Roy R Chaudhuri Lu Yu Fiona J E Morgan Simon Clare Jyoti Choudhary Nicholas R Thomson Pietro Lio Duncan J Maskell Pietro Mastroeni Andrew J Grant Transcriptome and proteome analysis of Salmonella enterica serovar Typhimurium systemic infection of wild type and immune-deficient mice. PLoS ONE |
author_facet |
Olusegun Oshota Max Conway Maria Fookes Fernanda Schreiber Roy R Chaudhuri Lu Yu Fiona J E Morgan Simon Clare Jyoti Choudhary Nicholas R Thomson Pietro Lio Duncan J Maskell Pietro Mastroeni Andrew J Grant |
author_sort |
Olusegun Oshota |
title |
Transcriptome and proteome analysis of Salmonella enterica serovar Typhimurium systemic infection of wild type and immune-deficient mice. |
title_short |
Transcriptome and proteome analysis of Salmonella enterica serovar Typhimurium systemic infection of wild type and immune-deficient mice. |
title_full |
Transcriptome and proteome analysis of Salmonella enterica serovar Typhimurium systemic infection of wild type and immune-deficient mice. |
title_fullStr |
Transcriptome and proteome analysis of Salmonella enterica serovar Typhimurium systemic infection of wild type and immune-deficient mice. |
title_full_unstemmed |
Transcriptome and proteome analysis of Salmonella enterica serovar Typhimurium systemic infection of wild type and immune-deficient mice. |
title_sort |
transcriptome and proteome analysis of salmonella enterica serovar typhimurium systemic infection of wild type and immune-deficient mice. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2017-01-01 |
description |
Salmonella enterica are a threat to public health. Current vaccines are not fully effective. The ability to grow in infected tissues within phagocytes is required for S. enterica virulence in systemic disease. As the infection progresses the bacteria are exposed to a complex host immune response. Consequently, in order to continue growing in the tissues, S. enterica requires the coordinated regulation of fitness genes. Bacterial gene regulation has so far been investigated largely using exposure to artificial environmental conditions or to in vitro cultured cells, and little information is available on how S. enterica adapts in vivo to sustain cell division and survival. We have studied the transcriptome, proteome and metabolic flux of Salmonella, and the transcriptome of the host during infection of wild type C57BL/6 and immune-deficient gp91-/-phox mice. Our analyses advance the understanding of how S. enterica and the host behaves during infection to a more sophisticated level than has previously been reported. |
url |
http://europepmc.org/articles/PMC5552096?pdf=render |
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