Local and systemic immunomodulatory mechanisms triggered by Human Papillomavirus transformed cells: a potential role for G-CSF and neutrophils
Abstract Cervical cancer is the last stage of a series of molecular and cellular alterations initiated with Human Papillomavirus (HPV) infection. The process involves immune responses and evasion mechanisms, which culminates with tolerance toward tumor antigens. Our objective was to understand local...
Main Authors: | , , , , , , , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2017-08-01
|
Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-017-09079-3 |
id |
doaj-1d9d6263aa184d768c0d46a69d067d8b |
---|---|
record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Karla Lucia Fernandez Alvarez Mariana Beldi Fabiane Sarmanho Renata Ariza Marques Rossetti Caio Raony Farina Silveira Giana Rabello Mota Maria Antonieta Andreoli Eliana Dias de Carvalho Caruso Marcia Ferreira Kamillos Ana Marta Souza Haydee Mastrocalla Maria Alejandra Clavijo-Salomon José Alexandre Marzagão Barbuto Noely Paula Lorenzi Adhemar Longatto-Filho Edmund Baracat Rossana Verónica Mendoza Lopez Luisa Lina Villa Maricy Tacla Ana Paula Lepique |
spellingShingle |
Karla Lucia Fernandez Alvarez Mariana Beldi Fabiane Sarmanho Renata Ariza Marques Rossetti Caio Raony Farina Silveira Giana Rabello Mota Maria Antonieta Andreoli Eliana Dias de Carvalho Caruso Marcia Ferreira Kamillos Ana Marta Souza Haydee Mastrocalla Maria Alejandra Clavijo-Salomon José Alexandre Marzagão Barbuto Noely Paula Lorenzi Adhemar Longatto-Filho Edmund Baracat Rossana Verónica Mendoza Lopez Luisa Lina Villa Maricy Tacla Ana Paula Lepique Local and systemic immunomodulatory mechanisms triggered by Human Papillomavirus transformed cells: a potential role for G-CSF and neutrophils Scientific Reports |
author_facet |
Karla Lucia Fernandez Alvarez Mariana Beldi Fabiane Sarmanho Renata Ariza Marques Rossetti Caio Raony Farina Silveira Giana Rabello Mota Maria Antonieta Andreoli Eliana Dias de Carvalho Caruso Marcia Ferreira Kamillos Ana Marta Souza Haydee Mastrocalla Maria Alejandra Clavijo-Salomon José Alexandre Marzagão Barbuto Noely Paula Lorenzi Adhemar Longatto-Filho Edmund Baracat Rossana Verónica Mendoza Lopez Luisa Lina Villa Maricy Tacla Ana Paula Lepique |
author_sort |
Karla Lucia Fernandez Alvarez |
title |
Local and systemic immunomodulatory mechanisms triggered by Human Papillomavirus transformed cells: a potential role for G-CSF and neutrophils |
title_short |
Local and systemic immunomodulatory mechanisms triggered by Human Papillomavirus transformed cells: a potential role for G-CSF and neutrophils |
title_full |
Local and systemic immunomodulatory mechanisms triggered by Human Papillomavirus transformed cells: a potential role for G-CSF and neutrophils |
title_fullStr |
Local and systemic immunomodulatory mechanisms triggered by Human Papillomavirus transformed cells: a potential role for G-CSF and neutrophils |
title_full_unstemmed |
Local and systemic immunomodulatory mechanisms triggered by Human Papillomavirus transformed cells: a potential role for G-CSF and neutrophils |
title_sort |
local and systemic immunomodulatory mechanisms triggered by human papillomavirus transformed cells: a potential role for g-csf and neutrophils |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-08-01 |
description |
Abstract Cervical cancer is the last stage of a series of molecular and cellular alterations initiated with Human Papillomavirus (HPV) infection. The process involves immune responses and evasion mechanisms, which culminates with tolerance toward tumor antigens. Our objective was to understand local and systemic changes in the interactions between HPV associated cervical lesions and the immune system as lesions progress to cancer. Locally, we observed higher cervical leukocyte infiltrate, reflected by the increase in the frequency of T lymphocytes, neutrophils and M2 macrophages, in cancer patients. We observed a strong negative correlation between the frequency of neutrophils and T cells in precursor and cancer samples, but not cervicitis. In 3D tumor cell cultures, neutrophils inhibited T cell activity, displayed longer viability and longer CD16 expression half-life than neat neutrophil cultures. Systemically, we observed higher plasma G-CSF concentration, higher frequency of immature low density neutrophils, and tolerogenic monocyte derived dendritic cells, MoDCs, also in cancer patients. Interestingly, there was a negative correlation between T cell activation by MoDCs and G-CSF concentration in the plasma. Our results indicate that neutrophils and G-CSF may be part of the immune escape mechanisms triggered by cervical cancer cells, locally and systemically, respectively. |
url |
https://doi.org/10.1038/s41598-017-09079-3 |
work_keys_str_mv |
AT karlaluciafernandezalvarez localandsystemicimmunomodulatorymechanismstriggeredbyhumanpapillomavirustransformedcellsapotentialroleforgcsfandneutrophils AT marianabeldi localandsystemicimmunomodulatorymechanismstriggeredbyhumanpapillomavirustransformedcellsapotentialroleforgcsfandneutrophils AT fabianesarmanho localandsystemicimmunomodulatorymechanismstriggeredbyhumanpapillomavirustransformedcellsapotentialroleforgcsfandneutrophils AT renataarizamarquesrossetti localandsystemicimmunomodulatorymechanismstriggeredbyhumanpapillomavirustransformedcellsapotentialroleforgcsfandneutrophils AT caioraonyfarinasilveira localandsystemicimmunomodulatorymechanismstriggeredbyhumanpapillomavirustransformedcellsapotentialroleforgcsfandneutrophils AT gianarabellomota localandsystemicimmunomodulatorymechanismstriggeredbyhumanpapillomavirustransformedcellsapotentialroleforgcsfandneutrophils AT mariaantonietaandreoli localandsystemicimmunomodulatorymechanismstriggeredbyhumanpapillomavirustransformedcellsapotentialroleforgcsfandneutrophils AT elianadiasdecarvalhocaruso localandsystemicimmunomodulatorymechanismstriggeredbyhumanpapillomavirustransformedcellsapotentialroleforgcsfandneutrophils AT marciaferreirakamillos localandsystemicimmunomodulatorymechanismstriggeredbyhumanpapillomavirustransformedcellsapotentialroleforgcsfandneutrophils AT anamartasouza localandsystemicimmunomodulatorymechanismstriggeredbyhumanpapillomavirustransformedcellsapotentialroleforgcsfandneutrophils AT haydeemastrocalla localandsystemicimmunomodulatorymechanismstriggeredbyhumanpapillomavirustransformedcellsapotentialroleforgcsfandneutrophils AT mariaalejandraclavijosalomon localandsystemicimmunomodulatorymechanismstriggeredbyhumanpapillomavirustransformedcellsapotentialroleforgcsfandneutrophils AT josealexandremarzagaobarbuto localandsystemicimmunomodulatorymechanismstriggeredbyhumanpapillomavirustransformedcellsapotentialroleforgcsfandneutrophils AT noelypaulalorenzi localandsystemicimmunomodulatorymechanismstriggeredbyhumanpapillomavirustransformedcellsapotentialroleforgcsfandneutrophils AT adhemarlongattofilho localandsystemicimmunomodulatorymechanismstriggeredbyhumanpapillomavirustransformedcellsapotentialroleforgcsfandneutrophils AT edmundbaracat localandsystemicimmunomodulatorymechanismstriggeredbyhumanpapillomavirustransformedcellsapotentialroleforgcsfandneutrophils AT rossanaveronicamendozalopez localandsystemicimmunomodulatorymechanismstriggeredbyhumanpapillomavirustransformedcellsapotentialroleforgcsfandneutrophils AT luisalinavilla localandsystemicimmunomodulatorymechanismstriggeredbyhumanpapillomavirustransformedcellsapotentialroleforgcsfandneutrophils AT maricytacla localandsystemicimmunomodulatorymechanismstriggeredbyhumanpapillomavirustransformedcellsapotentialroleforgcsfandneutrophils AT anapaulalepique localandsystemicimmunomodulatorymechanismstriggeredbyhumanpapillomavirustransformedcellsapotentialroleforgcsfandneutrophils |
_version_ |
1724395345908072448 |
spelling |
doaj-1d9d6263aa184d768c0d46a69d067d8b2020-12-08T01:02:14ZengNature Publishing GroupScientific Reports2045-23222017-08-017111610.1038/s41598-017-09079-3Local and systemic immunomodulatory mechanisms triggered by Human Papillomavirus transformed cells: a potential role for G-CSF and neutrophilsKarla Lucia Fernandez Alvarez0Mariana Beldi1Fabiane Sarmanho2Renata Ariza Marques Rossetti3Caio Raony Farina Silveira4Giana Rabello Mota5Maria Antonieta Andreoli6Eliana Dias de Carvalho Caruso7Marcia Ferreira Kamillos8Ana Marta Souza9Haydee Mastrocalla10Maria Alejandra Clavijo-Salomon11José Alexandre Marzagão Barbuto12Noely Paula Lorenzi13Adhemar Longatto-Filho14Edmund Baracat15Rossana Verónica Mendoza Lopez16Luisa Lina Villa17Maricy Tacla18Ana Paula Lepique19Department of Immunology, Institute of Biomedical Sciences, Universidade de São Paulo, Av. Prof. Lineu Prestes, 1730, Ed. Biomédicas IVDepartment of Gynecologic Clinic, School of Medicine, Universidade de São Paulo; Clinics Hospital at the São Paulo University, R. Dr. Enéas de Carvalho aguiar, 255, 5th floorDepartment of Gynecologic Clinic, School of Medicine, Universidade de São Paulo; Clinics Hospital at the São Paulo University, R. Dr. Enéas de Carvalho aguiar, 255, 5th floorDepartment of Immunology, Institute of Biomedical Sciences, Universidade de São Paulo, Av. Prof. Lineu Prestes, 1730, Ed. Biomédicas IVDepartment of Immunology, Institute of Biomedical Sciences, Universidade de São Paulo, Av. Prof. Lineu Prestes, 1730, Ed. Biomédicas IVDepartment of Radiology and Oncology, Faculdade de Medicina da Universidade de São Paulo, LIM-24. R. Dr. Ovídio Pires de Campos, 255, Radiology BuildingHospital AC Camargo, International Research Center, R. Taguá 440Department of Gynecologic Clinic, School of Medicine, Universidade de São Paulo; Clinics Hospital at the São Paulo University, R. Dr. Enéas de Carvalho aguiar, 255, 5th floorDepartment of Gynecologic Clinic, School of Medicine, Universidade de São Paulo; Clinics Hospital at the São Paulo University, R. Dr. Enéas de Carvalho aguiar, 255, 5th floorDepartment of Gynecologic Clinic, School of Medicine, Universidade de São Paulo; Clinics Hospital at the São Paulo University, R. Dr. Enéas de Carvalho aguiar, 255, 5th floorDepartment of Gynecologic Clinic, School of Medicine, Universidade de São Paulo; Clinics Hospital at the São Paulo University, R. Dr. Enéas de Carvalho aguiar, 255, 5th floorDepartment of Immunology, Institute of Biomedical Sciences, Universidade de São Paulo, Av. Prof. Lineu Prestes, 1730, Ed. Biomédicas IVDepartment of Immunology, Institute of Biomedical Sciences, Universidade de São Paulo, Av. Prof. Lineu Prestes, 1730, Ed. Biomédicas IVDepartment of Gynecologic Clinic, School of Medicine, Universidade de São Paulo; Clinics Hospital at the São Paulo University, R. Dr. Enéas de Carvalho aguiar, 255, 5th floorLaboratory of Medical Investigation, School of Medicine, University of São Paulo, Av. Dr. Arnaldo, 455, office 1159Department of Gynecologic Clinic, School of Medicine, Universidade de São Paulo; Clinics Hospital at the São Paulo University, R. Dr. Enéas de Carvalho aguiar, 255, 5th floorCenter for Translational Research in Oncology, Instituto do Câncer do Estado de São Paulo, Av. Dr. Arnaldo, 251, 8th floorDepartment of Radiology and Oncology, Faculdade de Medicina da Universidade de São Paulo, LIM-24. R. Dr. Ovídio Pires de Campos, 255, Radiology BuildingDepartment of Gynecologic Clinic, School of Medicine, Universidade de São Paulo; Clinics Hospital at the São Paulo University, R. Dr. Enéas de Carvalho aguiar, 255, 5th floorDepartment of Immunology, Institute of Biomedical Sciences, Universidade de São Paulo, Av. Prof. Lineu Prestes, 1730, Ed. Biomédicas IVAbstract Cervical cancer is the last stage of a series of molecular and cellular alterations initiated with Human Papillomavirus (HPV) infection. The process involves immune responses and evasion mechanisms, which culminates with tolerance toward tumor antigens. Our objective was to understand local and systemic changes in the interactions between HPV associated cervical lesions and the immune system as lesions progress to cancer. Locally, we observed higher cervical leukocyte infiltrate, reflected by the increase in the frequency of T lymphocytes, neutrophils and M2 macrophages, in cancer patients. We observed a strong negative correlation between the frequency of neutrophils and T cells in precursor and cancer samples, but not cervicitis. In 3D tumor cell cultures, neutrophils inhibited T cell activity, displayed longer viability and longer CD16 expression half-life than neat neutrophil cultures. Systemically, we observed higher plasma G-CSF concentration, higher frequency of immature low density neutrophils, and tolerogenic monocyte derived dendritic cells, MoDCs, also in cancer patients. Interestingly, there was a negative correlation between T cell activation by MoDCs and G-CSF concentration in the plasma. Our results indicate that neutrophils and G-CSF may be part of the immune escape mechanisms triggered by cervical cancer cells, locally and systemically, respectively.https://doi.org/10.1038/s41598-017-09079-3 |