Long term N-acetylcysteine administration rescues liver steatosis via endoplasmic reticulum stress with unfolded protein response in mice
Abstract Background Fat accumulation in the liver contributes to the development of non-alcoholic fatty liver disease (NAFLD). N-acetylcysteine (NAC) is an antioxidant, acting both directly and indirectly via upregulation of cellular antioxidants. We examined the mechanisms of liver steatosis after...
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doaj-1de87bdb7cfc4da1928fb8baf42f36302020-11-25T02:52:33ZengBMCLipids in Health and Disease1476-511X2020-05-0119111110.1186/s12944-020-01274-yLong term N-acetylcysteine administration rescues liver steatosis via endoplasmic reticulum stress with unfolded protein response in miceChing-Chou Tsai0Yu-Jen Chen1Hong-Ren Yu2Li-Tung Huang3You-Lin Tain4I-Chun Lin5Jiunn-Ming Sheen6Pei-Wen Wang7Mao-Meng Tiao8Department of Obstetrics and Gynecology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineDepartment of Obstetrics and Gynecology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineDepartment of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineDepartment of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineDepartment of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineDepartment of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineDepartment of Pediatrics, Chiayi Chang Gung Memorial HospitalDepartment of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineDepartment of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of MedicineAbstract Background Fat accumulation in the liver contributes to the development of non-alcoholic fatty liver disease (NAFLD). N-acetylcysteine (NAC) is an antioxidant, acting both directly and indirectly via upregulation of cellular antioxidants. We examined the mechanisms of liver steatosis after 12 months high fat (HF) diet and tested the ability of NAC to rescue liver steatosis. Methods Seven-week-old C57BL/6 (B6) male mice were administered HF diet for 12 months (HF group). Two other groups received HF diet for 12 months accompanied by NAC for 12 months (HFD + NAC(1–12)) or 6 months (HFD + NAC(1–6)). The control group was fed regular diet for 12 months (CD group). Results Liver steatosis was more pronounced in the HF group than in the CD group after 12 month feeding. NAC intake for 6 or 12 months decreased liver steatosis in comparison with HF diet (p < 0.05). Furthermore, NAC treatment also reduced cellular apoptosis and caspase-3 expression. In the unfolded protein response (UPR) pathway, the expression of ECHS1, HSP60, and HSP70 was decreased in the HFD group (p < 0.05) and rescued by NAC therapy. With regards to the endoplasmic reticulum (ER) stress, Phospho-PERK (p-PERK) and ATF4 expression was decreased in the HF group, and only the HFD + NAC(1–12), but not HFD + NAC(1–6) group, showed significant improvement. Conclusion HF diet for 12 months induces significant liver steatosis via altered ER stress and UPR pathway activity, as well as liver apoptosis. NAC treatment rescues the liver steatosis and apoptosis induced by HF diet.http://link.springer.com/article/10.1186/s12944-020-01274-yAcetylcysteineSteatosisLiverER stress |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ching-Chou Tsai Yu-Jen Chen Hong-Ren Yu Li-Tung Huang You-Lin Tain I-Chun Lin Jiunn-Ming Sheen Pei-Wen Wang Mao-Meng Tiao |
spellingShingle |
Ching-Chou Tsai Yu-Jen Chen Hong-Ren Yu Li-Tung Huang You-Lin Tain I-Chun Lin Jiunn-Ming Sheen Pei-Wen Wang Mao-Meng Tiao Long term N-acetylcysteine administration rescues liver steatosis via endoplasmic reticulum stress with unfolded protein response in mice Lipids in Health and Disease Acetylcysteine Steatosis Liver ER stress |
author_facet |
Ching-Chou Tsai Yu-Jen Chen Hong-Ren Yu Li-Tung Huang You-Lin Tain I-Chun Lin Jiunn-Ming Sheen Pei-Wen Wang Mao-Meng Tiao |
author_sort |
Ching-Chou Tsai |
title |
Long term N-acetylcysteine administration rescues liver steatosis via endoplasmic reticulum stress with unfolded protein response in mice |
title_short |
Long term N-acetylcysteine administration rescues liver steatosis via endoplasmic reticulum stress with unfolded protein response in mice |
title_full |
Long term N-acetylcysteine administration rescues liver steatosis via endoplasmic reticulum stress with unfolded protein response in mice |
title_fullStr |
Long term N-acetylcysteine administration rescues liver steatosis via endoplasmic reticulum stress with unfolded protein response in mice |
title_full_unstemmed |
Long term N-acetylcysteine administration rescues liver steatosis via endoplasmic reticulum stress with unfolded protein response in mice |
title_sort |
long term n-acetylcysteine administration rescues liver steatosis via endoplasmic reticulum stress with unfolded protein response in mice |
publisher |
BMC |
series |
Lipids in Health and Disease |
issn |
1476-511X |
publishDate |
2020-05-01 |
description |
Abstract Background Fat accumulation in the liver contributes to the development of non-alcoholic fatty liver disease (NAFLD). N-acetylcysteine (NAC) is an antioxidant, acting both directly and indirectly via upregulation of cellular antioxidants. We examined the mechanisms of liver steatosis after 12 months high fat (HF) diet and tested the ability of NAC to rescue liver steatosis. Methods Seven-week-old C57BL/6 (B6) male mice were administered HF diet for 12 months (HF group). Two other groups received HF diet for 12 months accompanied by NAC for 12 months (HFD + NAC(1–12)) or 6 months (HFD + NAC(1–6)). The control group was fed regular diet for 12 months (CD group). Results Liver steatosis was more pronounced in the HF group than in the CD group after 12 month feeding. NAC intake for 6 or 12 months decreased liver steatosis in comparison with HF diet (p < 0.05). Furthermore, NAC treatment also reduced cellular apoptosis and caspase-3 expression. In the unfolded protein response (UPR) pathway, the expression of ECHS1, HSP60, and HSP70 was decreased in the HFD group (p < 0.05) and rescued by NAC therapy. With regards to the endoplasmic reticulum (ER) stress, Phospho-PERK (p-PERK) and ATF4 expression was decreased in the HF group, and only the HFD + NAC(1–12), but not HFD + NAC(1–6) group, showed significant improvement. Conclusion HF diet for 12 months induces significant liver steatosis via altered ER stress and UPR pathway activity, as well as liver apoptosis. NAC treatment rescues the liver steatosis and apoptosis induced by HF diet. |
topic |
Acetylcysteine Steatosis Liver ER stress |
url |
http://link.springer.com/article/10.1186/s12944-020-01274-y |
work_keys_str_mv |
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