miR-486-5p attenuates tumor growth and lymphangiogenesis by targeting neuropilin-2 in colorectal carcinoma

• Main Authors: Liu C, Li M, Hu Y, Shi N, Yu H, Liu H, Lian H
• Format: Article
• Language: English
• Published: Dove Medical Press 2016-05-01
• Series: OncoTargets and Therapy
• Subjects: miR-486-5p; colorectal carcinoma; neuropilin-2; tumor growth; lymphangiogenesis
• Online Access: https://www.dovepress.com/mir-486-5p-attenuates-tumor-growth-and-lymphangiogenesis-by-targeting--peer-reviewed-article-OTT

Chengxia Liu, Ming Li, Yingbin Hu, Ning Shi, Haisheng Yu, Haiyan Liu, Haifeng Lian Department of Gastroenterology, the Affiliated Hospital of Binzhou Medical University, Binzhou, Shandong Province, People’s Republic of China Abstract: Increasing evidence suggests that microRNAs are associated with many important biologic processes in carcinogenesis. Despite ample research revealing the dysregualtion of miR-486-5p in various cancers, little is known about the roles of miR-486-5p in colorectal carcinoma (CRC). In this study, we investigated the biological functions and molecular mechanisms of miR-486-5p in CRC growth and invasion, discussing the potential of using miR-486-5p as a biomarker for colorectal cancers. Our data revealed that miR-486-5p was significantly downregulated in CRC tissues compared with the paracancer tissue by quantitative real-time polymerase chain reaction and that miR-486-5p was downregulated to a greater extent in advanced stage cancer (stage III and IV) as compared to early stage cancer (stage I and II). Luciferase reporter assay verified that neuropilin-2 was a direct functional target of miR-486-5p in the CRC cells, and upregulation of miR-486-5p in CRC cells negatively correlated with the expression of neuropilin-2. Furthermore, overexpression of miR-486-5p inhibited the tumor growth and lymphangiogenesis in nude mice, which was reversed by overexpression of neuropilin-2. Taken together, our study suggested miR-486-5p might be a suppressor of CRC. Keywords: miR-486-5p, colorectal carcinoma, neuropilin-2, tumor growth, lymphangiogenesis