Suppressive Effect and Molecular Mechanism of <i>Houttuynia cordata</i> Thunb. Extract against Prostate Carcinogenesis and Castration-Resistant Prostate Cancer
<i>Houttuynia cordata</i> Thunb. (HCT) is a well-known Asian medicinal plant with biological activities used in the treatment of many diseases including cancer. This study investigated the effects of HCT extract and its ethyl acetate fraction (EA) on prostate carcinogenesis and castratio...
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doaj-1e2e0c07c5ca498cbf051415669f9b912021-07-23T13:33:17ZengMDPI AGCancers2072-66942021-07-01133403340310.3390/cancers13143403Suppressive Effect and Molecular Mechanism of <i>Houttuynia cordata</i> Thunb. Extract against Prostate Carcinogenesis and Castration-Resistant Prostate CancerSubhawat Subhawa0Aya Naiki-Ito1Hiroyuki Kato2Taku Naiki3Masayuki Komura4Aya Nagano-Matsuo5Ranchana Yeewa6Shingo Inaguma7Teera Chewonarin8Ratana Banjerdpongchai9Satoru Takahashi10Department of Experimental Pathology and Tumor Biology, Nagoya City University Graduate School of Medical Sciences, 1-Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, JapanDepartment of Experimental Pathology and Tumor Biology, Nagoya City University Graduate School of Medical Sciences, 1-Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, JapanDepartment of Experimental Pathology and Tumor Biology, Nagoya City University Graduate School of Medical Sciences, 1-Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, JapanDepartment of Experimental Pathology and Tumor Biology, Nagoya City University Graduate School of Medical Sciences, 1-Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, JapanDepartment of Experimental Pathology and Tumor Biology, Nagoya City University Graduate School of Medical Sciences, 1-Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, JapanDepartment of Experimental Pathology and Tumor Biology, Nagoya City University Graduate School of Medical Sciences, 1-Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, JapanDepartment of Experimental Pathology and Tumor Biology, Nagoya City University Graduate School of Medical Sciences, 1-Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, JapanDepartment of Experimental Pathology and Tumor Biology, Nagoya City University Graduate School of Medical Sciences, 1-Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, JapanDepartment of Biochemistry, Faculty of Medicine, Chiang Mai University, 110 Intravaroros Rd., Sripoom, Muang, Chiang Mai 50200, ThailandDepartment of Biochemistry, Faculty of Medicine, Chiang Mai University, 110 Intravaroros Rd., Sripoom, Muang, Chiang Mai 50200, ThailandDepartment of Experimental Pathology and Tumor Biology, Nagoya City University Graduate School of Medical Sciences, 1-Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan<i>Houttuynia cordata</i> Thunb. (HCT) is a well-known Asian medicinal plant with biological activities used in the treatment of many diseases including cancer. This study investigated the effects of HCT extract and its ethyl acetate fraction (EA) on prostate carcinogenesis and castration-resistant prostate cancer (CRPC). HCT and EA induced apoptosis in androgen-sensitive prostate cancer cells (LNCaP) and CRPC cells (PCai1) through activation of caspases, down-regulation of androgen receptor, and inactivation of AKT/ERK/MAPK signaling. Rutin was found to be a major component in HCT (44.00 ± 5.61 mg/g) and EA (81.34 ± 5.21 mg/g) in a previous study. Rutin had similar effects to HCT/EA on LNCaP cells and was considered to be one of the active compounds. Moreover, HCT/EA inhibited cell migration and epithelial-mesenchymal transition phenotypes via STAT3/Snail/Twist pathways in LNCaP cells. The consumption of 1% HCT-mixed diet significantly decreased the incidence of adenocarcinoma in the lateral prostate lobe of the Transgenic rat for adenocarcinoma of prostate model. Similarly, tumor growth of PCai1 xenografts was significantly suppressed by 1% HCT treatment. HCT also induced caspase-dependent apoptosis via AKT inactivation in both in vivo models. Together, the results of in vitro and in vivo studies indicate that HCT has inhibitory effects against prostate carcinogenesis and CRPC. This plant therefore should receive more attention as a source for the future development of non-toxic chemopreventive agents against various cancers.https://www.mdpi.com/2072-6694/13/14/3403<i>Houttuynia cordata</i> Thunb.natural extractsfunctional foodanti-proliferationapoptosisprostate cancer |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Subhawat Subhawa Aya Naiki-Ito Hiroyuki Kato Taku Naiki Masayuki Komura Aya Nagano-Matsuo Ranchana Yeewa Shingo Inaguma Teera Chewonarin Ratana Banjerdpongchai Satoru Takahashi |
spellingShingle |
Subhawat Subhawa Aya Naiki-Ito Hiroyuki Kato Taku Naiki Masayuki Komura Aya Nagano-Matsuo Ranchana Yeewa Shingo Inaguma Teera Chewonarin Ratana Banjerdpongchai Satoru Takahashi Suppressive Effect and Molecular Mechanism of <i>Houttuynia cordata</i> Thunb. Extract against Prostate Carcinogenesis and Castration-Resistant Prostate Cancer Cancers <i>Houttuynia cordata</i> Thunb. natural extracts functional food anti-proliferation apoptosis prostate cancer |
author_facet |
Subhawat Subhawa Aya Naiki-Ito Hiroyuki Kato Taku Naiki Masayuki Komura Aya Nagano-Matsuo Ranchana Yeewa Shingo Inaguma Teera Chewonarin Ratana Banjerdpongchai Satoru Takahashi |
author_sort |
Subhawat Subhawa |
title |
Suppressive Effect and Molecular Mechanism of <i>Houttuynia cordata</i> Thunb. Extract against Prostate Carcinogenesis and Castration-Resistant Prostate Cancer |
title_short |
Suppressive Effect and Molecular Mechanism of <i>Houttuynia cordata</i> Thunb. Extract against Prostate Carcinogenesis and Castration-Resistant Prostate Cancer |
title_full |
Suppressive Effect and Molecular Mechanism of <i>Houttuynia cordata</i> Thunb. Extract against Prostate Carcinogenesis and Castration-Resistant Prostate Cancer |
title_fullStr |
Suppressive Effect and Molecular Mechanism of <i>Houttuynia cordata</i> Thunb. Extract against Prostate Carcinogenesis and Castration-Resistant Prostate Cancer |
title_full_unstemmed |
Suppressive Effect and Molecular Mechanism of <i>Houttuynia cordata</i> Thunb. Extract against Prostate Carcinogenesis and Castration-Resistant Prostate Cancer |
title_sort |
suppressive effect and molecular mechanism of <i>houttuynia cordata</i> thunb. extract against prostate carcinogenesis and castration-resistant prostate cancer |
publisher |
MDPI AG |
series |
Cancers |
issn |
2072-6694 |
publishDate |
2021-07-01 |
description |
<i>Houttuynia cordata</i> Thunb. (HCT) is a well-known Asian medicinal plant with biological activities used in the treatment of many diseases including cancer. This study investigated the effects of HCT extract and its ethyl acetate fraction (EA) on prostate carcinogenesis and castration-resistant prostate cancer (CRPC). HCT and EA induced apoptosis in androgen-sensitive prostate cancer cells (LNCaP) and CRPC cells (PCai1) through activation of caspases, down-regulation of androgen receptor, and inactivation of AKT/ERK/MAPK signaling. Rutin was found to be a major component in HCT (44.00 ± 5.61 mg/g) and EA (81.34 ± 5.21 mg/g) in a previous study. Rutin had similar effects to HCT/EA on LNCaP cells and was considered to be one of the active compounds. Moreover, HCT/EA inhibited cell migration and epithelial-mesenchymal transition phenotypes via STAT3/Snail/Twist pathways in LNCaP cells. The consumption of 1% HCT-mixed diet significantly decreased the incidence of adenocarcinoma in the lateral prostate lobe of the Transgenic rat for adenocarcinoma of prostate model. Similarly, tumor growth of PCai1 xenografts was significantly suppressed by 1% HCT treatment. HCT also induced caspase-dependent apoptosis via AKT inactivation in both in vivo models. Together, the results of in vitro and in vivo studies indicate that HCT has inhibitory effects against prostate carcinogenesis and CRPC. This plant therefore should receive more attention as a source for the future development of non-toxic chemopreventive agents against various cancers. |
topic |
<i>Houttuynia cordata</i> Thunb. natural extracts functional food anti-proliferation apoptosis prostate cancer |
url |
https://www.mdpi.com/2072-6694/13/14/3403 |
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