CD40L Expression Allows CD8+ T Cells to Promote Their Own Expansion and Differentiation through Dendritic Cells
CD8+ T cells play an important role in providing protective immunity against a wide range of pathogens, and a number of different factors control their activation. Although CD40L-mediated CD40 licensing of dendritic cells (DCs) by CD4+ T cells is known to be necessary for the generation of a robust...
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doaj-1e4221065f454e70988556de08ce40c02020-11-25T00:02:03ZengFrontiers Media S.A.Frontiers in Immunology1664-32242017-11-01810.3389/fimmu.2017.01484303774CD40L Expression Allows CD8+ T Cells to Promote Their Own Expansion and Differentiation through Dendritic CellsNeil Q. Tay0Neil Q. Tay1Neil Q. Tay2Debbie C. P. Lee3Debbie C. P. Lee4Yen Leong Chua5Yen Leong Chua6Nayana Prabhu7Nayana Prabhu8Nicholas R. J. Gascoigne9Nicholas R. J. Gascoigne10David M. Kemeny11David M. Kemeny12NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, SingaporeDepartment of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, SingaporeImmunology Programme, Life Sciences Institute, National University of Singapore, Singapore, SingaporeDepartment of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, SingaporeImmunology Programme, Life Sciences Institute, National University of Singapore, Singapore, SingaporeDepartment of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, SingaporeImmunology Programme, Life Sciences Institute, National University of Singapore, Singapore, SingaporeDepartment of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, SingaporeImmunology Programme, Life Sciences Institute, National University of Singapore, Singapore, SingaporeDepartment of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, SingaporeImmunology Programme, Life Sciences Institute, National University of Singapore, Singapore, SingaporeDepartment of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, SingaporeImmunology Programme, Life Sciences Institute, National University of Singapore, Singapore, SingaporeCD8+ T cells play an important role in providing protective immunity against a wide range of pathogens, and a number of different factors control their activation. Although CD40L-mediated CD40 licensing of dendritic cells (DCs) by CD4+ T cells is known to be necessary for the generation of a robust CD8+ T cell response, the contribution of CD8+ T cell-expressed CD40L on DC licensing is less clear. We have previously shown that CD8+ T cells are able to induce the production of IL-12 p70 by DCs in a CD40L-dependent manner, providing some evidence that CD8+ T cell-mediated activation of DCs is possible. To better understand the role of CD40L on CD8+ T cell responses, we generated and characterized CD40L-expressing CD8+ T cells both in vitro and in vivo. We found that CD40L was expressed on 30–50% of effector CD8+ T cells when stimulated and that this expression was transient. The expression of CD40L on CD8+ T cells promoted the proliferation and differentiation of both the CD40L-expressing CD8+ T cells and the bystander effector CD8+ T cells. This process occurred via a cell-extrinsic manner and was mediated by DCs. These data demonstrate the existence of a mechanism where CD8+ T cells and DCs cooperate to maximize CD8+ T cell responses.http://journal.frontiersin.org/article/10.3389/fimmu.2017.01484/fullCD8+ T cellsCD40CD40Ldendritic cellscell proliferationcell differentiation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Neil Q. Tay Neil Q. Tay Neil Q. Tay Debbie C. P. Lee Debbie C. P. Lee Yen Leong Chua Yen Leong Chua Nayana Prabhu Nayana Prabhu Nicholas R. J. Gascoigne Nicholas R. J. Gascoigne David M. Kemeny David M. Kemeny |
spellingShingle |
Neil Q. Tay Neil Q. Tay Neil Q. Tay Debbie C. P. Lee Debbie C. P. Lee Yen Leong Chua Yen Leong Chua Nayana Prabhu Nayana Prabhu Nicholas R. J. Gascoigne Nicholas R. J. Gascoigne David M. Kemeny David M. Kemeny CD40L Expression Allows CD8+ T Cells to Promote Their Own Expansion and Differentiation through Dendritic Cells Frontiers in Immunology CD8+ T cells CD40 CD40L dendritic cells cell proliferation cell differentiation |
author_facet |
Neil Q. Tay Neil Q. Tay Neil Q. Tay Debbie C. P. Lee Debbie C. P. Lee Yen Leong Chua Yen Leong Chua Nayana Prabhu Nayana Prabhu Nicholas R. J. Gascoigne Nicholas R. J. Gascoigne David M. Kemeny David M. Kemeny |
author_sort |
Neil Q. Tay |
title |
CD40L Expression Allows CD8+ T Cells to Promote Their Own Expansion and Differentiation through Dendritic Cells |
title_short |
CD40L Expression Allows CD8+ T Cells to Promote Their Own Expansion and Differentiation through Dendritic Cells |
title_full |
CD40L Expression Allows CD8+ T Cells to Promote Their Own Expansion and Differentiation through Dendritic Cells |
title_fullStr |
CD40L Expression Allows CD8+ T Cells to Promote Their Own Expansion and Differentiation through Dendritic Cells |
title_full_unstemmed |
CD40L Expression Allows CD8+ T Cells to Promote Their Own Expansion and Differentiation through Dendritic Cells |
title_sort |
cd40l expression allows cd8+ t cells to promote their own expansion and differentiation through dendritic cells |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2017-11-01 |
description |
CD8+ T cells play an important role in providing protective immunity against a wide range of pathogens, and a number of different factors control their activation. Although CD40L-mediated CD40 licensing of dendritic cells (DCs) by CD4+ T cells is known to be necessary for the generation of a robust CD8+ T cell response, the contribution of CD8+ T cell-expressed CD40L on DC licensing is less clear. We have previously shown that CD8+ T cells are able to induce the production of IL-12 p70 by DCs in a CD40L-dependent manner, providing some evidence that CD8+ T cell-mediated activation of DCs is possible. To better understand the role of CD40L on CD8+ T cell responses, we generated and characterized CD40L-expressing CD8+ T cells both in vitro and in vivo. We found that CD40L was expressed on 30–50% of effector CD8+ T cells when stimulated and that this expression was transient. The expression of CD40L on CD8+ T cells promoted the proliferation and differentiation of both the CD40L-expressing CD8+ T cells and the bystander effector CD8+ T cells. This process occurred via a cell-extrinsic manner and was mediated by DCs. These data demonstrate the existence of a mechanism where CD8+ T cells and DCs cooperate to maximize CD8+ T cell responses. |
topic |
CD8+ T cells CD40 CD40L dendritic cells cell proliferation cell differentiation |
url |
http://journal.frontiersin.org/article/10.3389/fimmu.2017.01484/full |
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