Proteomic identification of galectin-11 and 14 ligands from Haemonchus contortus
Haemonchus contortus is the most pathogenic nematode of small ruminants. Infection in sheep and goats results in anaemia that decreases animal productivity and can ultimately cause death. The involvement of ruminant-specific galectin-11 (LGALS-11) and galectin-14 (LGALS-14) has been postulated to pl...
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doaj-1e54bd8f8c864e6aa00d04b7c0308c492020-11-25T00:15:31ZengPeerJ Inc.PeerJ2167-83592018-03-016e451010.7717/peerj.4510Proteomic identification of galectin-11 and 14 ligands from Haemonchus contortusDhanasekaran Sakthivel0Jaclyn Swan1Sarah Preston2MD Shakif-Azam3Pierre Faou4Yaqing Jiao5Rachael Downs6Harinda Rajapaksha7Robin Gasser8David Piedrafita9Travis Beddoe10Department of Animal, Plant and Soil Science and Centre for AgriBioscience (AgriBio), La Trobe University, Bundoora, Victoria, AustraliaDepartment of Animal, Plant and Soil Science and Centre for AgriBioscience (AgriBio), La Trobe University, Bundoora, Victoria, AustraliaMelbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Melbourne, AustraliaSchool of Applied and Biomedical Sciences, Federation University, Churchill, AustraliaDepartment of Biochemistry & Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, AustraliaMelbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Melbourne, AustraliaDepartment of Biochemistry & Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, AustraliaDepartment of Biochemistry & Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, AustraliaMelbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Melbourne, AustraliaSchool of Applied and Biomedical Sciences, Federation University, Churchill, AustraliaDepartment of Animal, Plant and Soil Science and Centre for AgriBioscience (AgriBio), La Trobe University, Bundoora, Victoria, AustraliaHaemonchus contortus is the most pathogenic nematode of small ruminants. Infection in sheep and goats results in anaemia that decreases animal productivity and can ultimately cause death. The involvement of ruminant-specific galectin-11 (LGALS-11) and galectin-14 (LGALS-14) has been postulated to play important roles in protective immune responses against parasitic infection; however, their ligands are unknown. In the current study, LGALS-11 and LGALS-14 ligands in H. contortus were identified from larval (L4) and adult parasitic stages extracts using immobilised LGALS-11 and LGALS-14 affinity column chromatography and mass spectrometry. Both LGALS-11 and LGALS-14 bound more putative protein targets in the adult stage of H. contortus (43 proteins) when compared to the larval stage (two proteins). Of the 43 proteins identified in the adult stage, 34 and 35 proteins were bound by LGALS-11 and LGALS-14, respectively, with 26 proteins binding to both galectins. Interestingly, hematophagous stage-specific sperm-coating protein and zinc metalloprotease (M13), which are known vaccine candidates, were identified as putative ligands of both LGALS-11 and LGALS-14. The identification of glycoproteins of H. contortus by LGALS-11 and LGALS-14 provide new insights into host-parasite interactions and the potential for developing new interventions.https://peerj.com/articles/4510.pdfMass spectrometryHost-parasite interactomeGalectin-14Haemonchus contortusGalectin-11Gastrointestinal parasite |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dhanasekaran Sakthivel Jaclyn Swan Sarah Preston MD Shakif-Azam Pierre Faou Yaqing Jiao Rachael Downs Harinda Rajapaksha Robin Gasser David Piedrafita Travis Beddoe |
spellingShingle |
Dhanasekaran Sakthivel Jaclyn Swan Sarah Preston MD Shakif-Azam Pierre Faou Yaqing Jiao Rachael Downs Harinda Rajapaksha Robin Gasser David Piedrafita Travis Beddoe Proteomic identification of galectin-11 and 14 ligands from Haemonchus contortus PeerJ Mass spectrometry Host-parasite interactome Galectin-14 Haemonchus contortus Galectin-11 Gastrointestinal parasite |
author_facet |
Dhanasekaran Sakthivel Jaclyn Swan Sarah Preston MD Shakif-Azam Pierre Faou Yaqing Jiao Rachael Downs Harinda Rajapaksha Robin Gasser David Piedrafita Travis Beddoe |
author_sort |
Dhanasekaran Sakthivel |
title |
Proteomic identification of galectin-11 and 14 ligands from Haemonchus contortus |
title_short |
Proteomic identification of galectin-11 and 14 ligands from Haemonchus contortus |
title_full |
Proteomic identification of galectin-11 and 14 ligands from Haemonchus contortus |
title_fullStr |
Proteomic identification of galectin-11 and 14 ligands from Haemonchus contortus |
title_full_unstemmed |
Proteomic identification of galectin-11 and 14 ligands from Haemonchus contortus |
title_sort |
proteomic identification of galectin-11 and 14 ligands from haemonchus contortus |
publisher |
PeerJ Inc. |
series |
PeerJ |
issn |
2167-8359 |
publishDate |
2018-03-01 |
description |
Haemonchus contortus is the most pathogenic nematode of small ruminants. Infection in sheep and goats results in anaemia that decreases animal productivity and can ultimately cause death. The involvement of ruminant-specific galectin-11 (LGALS-11) and galectin-14 (LGALS-14) has been postulated to play important roles in protective immune responses against parasitic infection; however, their ligands are unknown. In the current study, LGALS-11 and LGALS-14 ligands in H. contortus were identified from larval (L4) and adult parasitic stages extracts using immobilised LGALS-11 and LGALS-14 affinity column chromatography and mass spectrometry. Both LGALS-11 and LGALS-14 bound more putative protein targets in the adult stage of H. contortus (43 proteins) when compared to the larval stage (two proteins). Of the 43 proteins identified in the adult stage, 34 and 35 proteins were bound by LGALS-11 and LGALS-14, respectively, with 26 proteins binding to both galectins. Interestingly, hematophagous stage-specific sperm-coating protein and zinc metalloprotease (M13), which are known vaccine candidates, were identified as putative ligands of both LGALS-11 and LGALS-14. The identification of glycoproteins of H. contortus by LGALS-11 and LGALS-14 provide new insights into host-parasite interactions and the potential for developing new interventions. |
topic |
Mass spectrometry Host-parasite interactome Galectin-14 Haemonchus contortus Galectin-11 Gastrointestinal parasite |
url |
https://peerj.com/articles/4510.pdf |
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