GluLa-DPG-PEG600 nanopolymer binds proteins and spreads to rats’ organs and tissues

• Main Authors: B. O. Chekh, M. V. Ferens, Y. V. Martyn, D. D. Ostapiv, V. V. Vlizlo
• Format: Article
• Language: English
• Published: Львівський національний університет імені Івана Франка 2016-12-01
• Series: Біологічні студії
• Subjects: rats; nanopolymer; pseudopolyamino acids; drug transporter; adjuvant
• Online Access: http://publications.lnu.edu.ua/journals/index.php/biology/article/view/25
• ISSN: 1996-4536; 2311-0783

In this article, we described the ability of GluLa-DPG-PEG600 nanopolymer based on the pseudopolyamino acids to bind proteins and its localization in rats organs and tissues after intravenous and intramuscular injections. By using electrophoresis in 5% polyacrylamide gel, it was found the ability of GluLa-DPG-PEG600 nanopolymer to bind bovine serum albumin (BSA). This indicates the GluLa-DPG-PEG600 is a potential transporter of proteins and their complexes. By means of fluorescent microscopy, it was found that GluLa-DPG-PEG600 nanopolymer labeled with fluorescein with BSA labeled with fluorescent Alexa Fluor 555 dye (GluLa-DPG-PEG600-F + BSA Alexa Fluor 555) localized in liver and brain on the 16th after intramuscular injection. On the 5th hour after intravenous injection, GluLa-DPG-PEG600-F + BSA Alexa Fluor 555 complex localized in spleen and kidney obtained. Results show a potential usefulness of GluLa-DPG-PEG600 as a transporter of drugs capable of penetrating the blood-brain barrier. Locali­zation of GluLa-DPG-PEG600-F + BSA Alexa Fluor 555 complex in spleen suggests that GluLa-DPG-PEG600 could be used as an adjuvant for development of vaccines.