Chemical Profiling, Antioxidant, Anticholinesterase, and Antiprotozoal Potentials of Artemisia copa Phil. (Asteraceae)
Artemisia copa Phil. (Asteraceae) (known as copa-copa) is a native species of Chile used as an infusion in traditional medicine by Atacameños people in the Altiplano, highlands of northern Chile. In this research, we have investigated for the first time the cholinesterase inhibition potential agains...
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doaj-1e6e6ed4b33344dea7ba04e2b077a6082020-12-08T08:34:37ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122020-12-011110.3389/fphar.2020.594174594174Chemical Profiling, Antioxidant, Anticholinesterase, and Antiprotozoal Potentials of Artemisia copa Phil. (Asteraceae)María José Larrazábal-Fuentes0Carlos Fernández-Galleguillos1Jenifer Palma-Ramírez2Javier Romero-Parra3Kevin Sepúlveda4Alexandra Galetovic5Jorge González6Adrián Paredes7Jorge Bórquez8Mario J. Simirgiotis9Javier Echeverría10Unidad Alimentos, Departamento de Ciencias de los Alimentos y Nutrición, Facultad de Ciencias de la Salud, Universidad de Antofagasta, Antofagasta, ChileInstituto de Farmacia, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, ChileUnidad Alimentos, Departamento de Ciencias de los Alimentos y Nutrición, Facultad de Ciencias de la Salud, Universidad de Antofagasta, Antofagasta, ChileDepartamento de Química Orgánica y Fisicoquímica, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago, ChileUnidad de Parasitología Molecular, Departamento de Tecnología Médica, Facultad de Ciencias de la Salud, Universidad de Antofagasta, Antofagasta, ChileDepartamento Biomédico, Universidad de Antofagasta, Antofagasta, ChileUnidad de Parasitología Molecular, Departamento de Tecnología Médica, Facultad de Ciencias de la Salud, Universidad de Antofagasta, Antofagasta, ChileLaboratorio de Productos Naturales, Departamento de Química, Facultad de Ciencias Básicas, Universidad de Antofagasta, Antofagasta, ChileLaboratorio de Productos Naturales, Departamento de Química, Facultad de Ciencias Básicas, Universidad de Antofagasta, Antofagasta, ChileInstituto de Farmacia, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, ChileDepartamento de Ciencias del Ambiente, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, ChileArtemisia copa Phil. (Asteraceae) (known as copa-copa) is a native species of Chile used as an infusion in traditional medicine by Atacameños people in the Altiplano, highlands of northern Chile. In this research, we have investigated for the first time the cholinesterase inhibition potential against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), and the chemical profiling of the infusions prepared from the aerial parts of A. copa by high resolution spectrometry. In addition, total phenolic, total flavonoid content, antioxidant (DPPH, FRAP, and ORAC) and antiprozoal activity were tested. Artemisia copa showed good inhibitory activity against AChE and BChE (3.92 ± 0.08 µg/ml and 44.13 ± 0.10 µg/ml). The infusion displayed a total phenolics content of 155.6 ± 2.9 mg of gallic acid equivalents/g and total flavonoid content of 5.5 ± 0.2 mg quercetin equivalents/g. Additionally, trypanocidal activity against Trypanosoma cruzi was found (LD50 of 131.8 µg/ml). Forty-seven metabolites were detected in the infusion of A. copa including several phenolic acids and flavonoids which were rapidly identified using ultrahigh performance liquid chromatography orbitrap mass spectrometry analysis (UHPLC-Orbitrap-MS) for chemical profiling. The major compounds identified in the infusions were studied by molecular docking against AChE and BChE. The UHPLC-MS fingerprints generated can be also used for the authentication of these endemic species. These findings reveal that A. copa infusions can be used as beverages with protective effects.https://www.frontiersin.org/articles/10.3389/fphar.2020.594174/fullArtemisia copaAsteraceaetraditional medicineHPLC-MSanti-Trypanosoma cruziAnti-Trypanosoma activity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
María José Larrazábal-Fuentes Carlos Fernández-Galleguillos Jenifer Palma-Ramírez Javier Romero-Parra Kevin Sepúlveda Alexandra Galetovic Jorge González Adrián Paredes Jorge Bórquez Mario J. Simirgiotis Javier Echeverría |
spellingShingle |
María José Larrazábal-Fuentes Carlos Fernández-Galleguillos Jenifer Palma-Ramírez Javier Romero-Parra Kevin Sepúlveda Alexandra Galetovic Jorge González Adrián Paredes Jorge Bórquez Mario J. Simirgiotis Javier Echeverría Chemical Profiling, Antioxidant, Anticholinesterase, and Antiprotozoal Potentials of Artemisia copa Phil. (Asteraceae) Frontiers in Pharmacology Artemisia copa Asteraceae traditional medicine HPLC-MS anti-Trypanosoma cruzi Anti-Trypanosoma activity |
author_facet |
María José Larrazábal-Fuentes Carlos Fernández-Galleguillos Jenifer Palma-Ramírez Javier Romero-Parra Kevin Sepúlveda Alexandra Galetovic Jorge González Adrián Paredes Jorge Bórquez Mario J. Simirgiotis Javier Echeverría |
author_sort |
María José Larrazábal-Fuentes |
title |
Chemical Profiling, Antioxidant, Anticholinesterase, and Antiprotozoal Potentials of Artemisia copa Phil. (Asteraceae) |
title_short |
Chemical Profiling, Antioxidant, Anticholinesterase, and Antiprotozoal Potentials of Artemisia copa Phil. (Asteraceae) |
title_full |
Chemical Profiling, Antioxidant, Anticholinesterase, and Antiprotozoal Potentials of Artemisia copa Phil. (Asteraceae) |
title_fullStr |
Chemical Profiling, Antioxidant, Anticholinesterase, and Antiprotozoal Potentials of Artemisia copa Phil. (Asteraceae) |
title_full_unstemmed |
Chemical Profiling, Antioxidant, Anticholinesterase, and Antiprotozoal Potentials of Artemisia copa Phil. (Asteraceae) |
title_sort |
chemical profiling, antioxidant, anticholinesterase, and antiprotozoal potentials of artemisia copa phil. (asteraceae) |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Pharmacology |
issn |
1663-9812 |
publishDate |
2020-12-01 |
description |
Artemisia copa Phil. (Asteraceae) (known as copa-copa) is a native species of Chile used as an infusion in traditional medicine by Atacameños people in the Altiplano, highlands of northern Chile. In this research, we have investigated for the first time the cholinesterase inhibition potential against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), and the chemical profiling of the infusions prepared from the aerial parts of A. copa by high resolution spectrometry. In addition, total phenolic, total flavonoid content, antioxidant (DPPH, FRAP, and ORAC) and antiprozoal activity were tested. Artemisia copa showed good inhibitory activity against AChE and BChE (3.92 ± 0.08 µg/ml and 44.13 ± 0.10 µg/ml). The infusion displayed a total phenolics content of 155.6 ± 2.9 mg of gallic acid equivalents/g and total flavonoid content of 5.5 ± 0.2 mg quercetin equivalents/g. Additionally, trypanocidal activity against Trypanosoma cruzi was found (LD50 of 131.8 µg/ml). Forty-seven metabolites were detected in the infusion of A. copa including several phenolic acids and flavonoids which were rapidly identified using ultrahigh performance liquid chromatography orbitrap mass spectrometry analysis (UHPLC-Orbitrap-MS) for chemical profiling. The major compounds identified in the infusions were studied by molecular docking against AChE and BChE. The UHPLC-MS fingerprints generated can be also used for the authentication of these endemic species. These findings reveal that A. copa infusions can be used as beverages with protective effects. |
topic |
Artemisia copa Asteraceae traditional medicine HPLC-MS anti-Trypanosoma cruzi Anti-Trypanosoma activity |
url |
https://www.frontiersin.org/articles/10.3389/fphar.2020.594174/full |
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