Hydroethanolic Extract of Strychnos pseudoquina Accelerates Skin Wound Healing by Modulating the Oxidative Status and Microstructural Reorganization of Scar Tissue in Experimental Type I Diabetes
The effect of topical application of ointment based on Strychnos pseudoquina hydroethanolic extract in the cutaneous wounds healing in diabetic rats was evaluated. Samples of S. pseudoquina were submitted to phytochemical prospection and in vitro antioxidant assay. Thirty Wistar rats were divided in...
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doaj-1e7718a9ea55481c9ae215a9436e844d2020-11-24T23:19:33ZengHindawi LimitedBioMed Research International2314-61332314-61412017-01-01201710.1155/2017/95383519538351Hydroethanolic Extract of Strychnos pseudoquina Accelerates Skin Wound Healing by Modulating the Oxidative Status and Microstructural Reorganization of Scar Tissue in Experimental Type I DiabetesMariáurea M. Sarandy0Rômulo D. Novaes1Antônio A. Xavier2Camilo E. Vital3João P. V. Leite4Fabiana C. S. A. Melo5Reggiani V. Gonçalves6Department of General Biology, Federal University of Viçosa, 35570-000 Viçosa, MG, BrazilInstitute of Biomedical Sciences, Department of Structural Biology, Federal University of Alfenas, 37130-001 Alfenas, MG, BrazilDepartment of Biochemistry and Molecular Biology, Federal University of Viçosa, 35570-000 Viçosa, MG, BrazilCenter of Biomolecules Analysis (NuBioMol), Federal University of Viçosa, 35570-000 Viçosa, MG, BrazilDepartment of Biochemistry and Molecular Biology, Federal University of Viçosa, 35570-000 Viçosa, MG, BrazilDepartment of Animal Biology, Federal University of Viçosa, 35570-000 Viçosa, MG, BrazilDepartment of Animal Biology, Federal University of Viçosa, 35570-000 Viçosa, MG, BrazilThe effect of topical application of ointment based on Strychnos pseudoquina hydroethanolic extract in the cutaneous wounds healing in diabetic rats was evaluated. Samples of S. pseudoquina were submitted to phytochemical prospection and in vitro antioxidant assay. Thirty Wistar rats were divided into 5 groups: Sal-wounds treated with 0.9% saline solution; VH-wounds treated with 0.6 g of lanolin cream (vehicle); SS-wounds treated with silver sulfadiazine cream (10 mg/g); ES5- and ES10-wounds treated with an ointment of S. pseudoquina extract, 5% and 10%, respectively. Fragments of wounds were removed for histological and biochemical analysis every 7 days during 21 days. ES showed equivalent levels per gram of extract of total phenols and flavonoids equal to 122.04 mg for TAE and 0.60 mg for RE. The chlorogenic acid was one of the major constituents. S. pseudoquina extract presented high antioxidant potential in vitro. ES5 and ES10 showed higher wound healing rate and higher amount of cells, blood vessels, and type III and I collagen. The oxidative stress markers were lower in the ES5 and ES10 groups, while the antioxidants enzymes levels were higher. Ointment based on S. pseudoquina extract promotes a fast and efficient cutaneous repair in diabetic rats.http://dx.doi.org/10.1155/2017/9538351 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mariáurea M. Sarandy Rômulo D. Novaes Antônio A. Xavier Camilo E. Vital João P. V. Leite Fabiana C. S. A. Melo Reggiani V. Gonçalves |
spellingShingle |
Mariáurea M. Sarandy Rômulo D. Novaes Antônio A. Xavier Camilo E. Vital João P. V. Leite Fabiana C. S. A. Melo Reggiani V. Gonçalves Hydroethanolic Extract of Strychnos pseudoquina Accelerates Skin Wound Healing by Modulating the Oxidative Status and Microstructural Reorganization of Scar Tissue in Experimental Type I Diabetes BioMed Research International |
author_facet |
Mariáurea M. Sarandy Rômulo D. Novaes Antônio A. Xavier Camilo E. Vital João P. V. Leite Fabiana C. S. A. Melo Reggiani V. Gonçalves |
author_sort |
Mariáurea M. Sarandy |
title |
Hydroethanolic Extract of Strychnos pseudoquina Accelerates Skin Wound Healing by Modulating the Oxidative Status and Microstructural Reorganization of Scar Tissue in Experimental Type I Diabetes |
title_short |
Hydroethanolic Extract of Strychnos pseudoquina Accelerates Skin Wound Healing by Modulating the Oxidative Status and Microstructural Reorganization of Scar Tissue in Experimental Type I Diabetes |
title_full |
Hydroethanolic Extract of Strychnos pseudoquina Accelerates Skin Wound Healing by Modulating the Oxidative Status and Microstructural Reorganization of Scar Tissue in Experimental Type I Diabetes |
title_fullStr |
Hydroethanolic Extract of Strychnos pseudoquina Accelerates Skin Wound Healing by Modulating the Oxidative Status and Microstructural Reorganization of Scar Tissue in Experimental Type I Diabetes |
title_full_unstemmed |
Hydroethanolic Extract of Strychnos pseudoquina Accelerates Skin Wound Healing by Modulating the Oxidative Status and Microstructural Reorganization of Scar Tissue in Experimental Type I Diabetes |
title_sort |
hydroethanolic extract of strychnos pseudoquina accelerates skin wound healing by modulating the oxidative status and microstructural reorganization of scar tissue in experimental type i diabetes |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2017-01-01 |
description |
The effect of topical application of ointment based on Strychnos pseudoquina hydroethanolic extract in the cutaneous wounds healing in diabetic rats was evaluated. Samples of S. pseudoquina were submitted to phytochemical prospection and in vitro antioxidant assay. Thirty Wistar rats were divided into 5 groups: Sal-wounds treated with 0.9% saline solution; VH-wounds treated with 0.6 g of lanolin cream (vehicle); SS-wounds treated with silver sulfadiazine cream (10 mg/g); ES5- and ES10-wounds treated with an ointment of S. pseudoquina extract, 5% and 10%, respectively. Fragments of wounds were removed for histological and biochemical analysis every 7 days during 21 days. ES showed equivalent levels per gram of extract of total phenols and flavonoids equal to 122.04 mg for TAE and 0.60 mg for RE. The chlorogenic acid was one of the major constituents. S. pseudoquina extract presented high antioxidant potential in vitro. ES5 and ES10 showed higher wound healing rate and higher amount of cells, blood vessels, and type III and I collagen. The oxidative stress markers were lower in the ES5 and ES10 groups, while the antioxidants enzymes levels were higher. Ointment based on S. pseudoquina extract promotes a fast and efficient cutaneous repair in diabetic rats. |
url |
http://dx.doi.org/10.1155/2017/9538351 |
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