Gynura procumbens Improved Fertility of Diabetic Rats: Preliminary Study of Sperm Proteomic
Gynura procumbens (GP) is a medicinal herb that has long been known as anti-inflammatory and antihyperglycaemic. Recently, this herbal extract has been associated with a profertility effect, suggesting its applicability in treating both diabetes and male infertility. In this study, the effects of GP...
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Online Access: | http://dx.doi.org/10.1155/2018/9201539 |
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doaj-1e7a3bcb83d64813b8ee1f1d21aecc192020-11-24T23:56:30ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882018-01-01201810.1155/2018/92015399201539Gynura procumbens Improved Fertility of Diabetic Rats: Preliminary Study of Sperm ProteomicKhaidatul Akmar Kamaruzaman0Wan Mohd Aizat1Mahanem Mat Noor2Centre for Biotechnology and Functional Food, Faculty of Science and Technology, Universiti Kebangsaan Malaysia (UKM), 43600 Bangi, Selangor, MalaysiaInstitute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia (UKM), 43600 Bangi, Selangor, MalaysiaCentre for Biotechnology and Functional Food, Faculty of Science and Technology, Universiti Kebangsaan Malaysia (UKM), 43600 Bangi, Selangor, MalaysiaGynura procumbens (GP) is a medicinal herb that has long been known as anti-inflammatory and antihyperglycaemic. Recently, this herbal extract has been associated with a profertility effect, suggesting its applicability in treating both diabetes and male infertility. In this study, the effects of GP aqueous extract (GPAE) on diabetic rats were investigated through evaluating testes histology and androgen hormone levels as well as the implantation sites of female rats on copulation with the treated male rats. Three dosages of GPAE were used (150, 300, and 450 mg/kg), and there were three control groups [normal, diabetic, and metformin-treated diabetic]. Testes histology, androgen hormone levels, and number of implantation sites of the GPAE-treated groups matched those of the normal group in contrast to the diabetic and metformin-treated diabetic controls. Sperm proteomics analysis identified 666 proteins, but only 88 were consistently found in all the control and 450-mg/kg GPAE-treated groups. Four proteins, including cysteine-rich secretory protein 1, carboxylesterase 5A, zona pellucida binding protein, and phosphatidylethanolamine-binding protein 1, were significantly upregulated with GPAE treatment compared with the diabetic control, matching the protein levels of the normal group. These proteins were mainly involved in sperm maturation, sperm capacitation, and sperm-egg interaction, suggesting that GP treatment was able to restore the fertility of male diabetic rats at molecular protein level. In conclusion, GP treatment effectively treats infertility of male diabetic rats, possibly through the upregulation of proteins related to sperm maturation and sperm-egg interaction.http://dx.doi.org/10.1155/2018/9201539 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Khaidatul Akmar Kamaruzaman Wan Mohd Aizat Mahanem Mat Noor |
spellingShingle |
Khaidatul Akmar Kamaruzaman Wan Mohd Aizat Mahanem Mat Noor Gynura procumbens Improved Fertility of Diabetic Rats: Preliminary Study of Sperm Proteomic Evidence-Based Complementary and Alternative Medicine |
author_facet |
Khaidatul Akmar Kamaruzaman Wan Mohd Aizat Mahanem Mat Noor |
author_sort |
Khaidatul Akmar Kamaruzaman |
title |
Gynura procumbens Improved Fertility of Diabetic Rats: Preliminary Study of Sperm Proteomic |
title_short |
Gynura procumbens Improved Fertility of Diabetic Rats: Preliminary Study of Sperm Proteomic |
title_full |
Gynura procumbens Improved Fertility of Diabetic Rats: Preliminary Study of Sperm Proteomic |
title_fullStr |
Gynura procumbens Improved Fertility of Diabetic Rats: Preliminary Study of Sperm Proteomic |
title_full_unstemmed |
Gynura procumbens Improved Fertility of Diabetic Rats: Preliminary Study of Sperm Proteomic |
title_sort |
gynura procumbens improved fertility of diabetic rats: preliminary study of sperm proteomic |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2018-01-01 |
description |
Gynura procumbens (GP) is a medicinal herb that has long been known as anti-inflammatory and antihyperglycaemic. Recently, this herbal extract has been associated with a profertility effect, suggesting its applicability in treating both diabetes and male infertility. In this study, the effects of GP aqueous extract (GPAE) on diabetic rats were investigated through evaluating testes histology and androgen hormone levels as well as the implantation sites of female rats on copulation with the treated male rats. Three dosages of GPAE were used (150, 300, and 450 mg/kg), and there were three control groups [normal, diabetic, and metformin-treated diabetic]. Testes histology, androgen hormone levels, and number of implantation sites of the GPAE-treated groups matched those of the normal group in contrast to the diabetic and metformin-treated diabetic controls. Sperm proteomics analysis identified 666 proteins, but only 88 were consistently found in all the control and 450-mg/kg GPAE-treated groups. Four proteins, including cysteine-rich secretory protein 1, carboxylesterase 5A, zona pellucida binding protein, and phosphatidylethanolamine-binding protein 1, were significantly upregulated with GPAE treatment compared with the diabetic control, matching the protein levels of the normal group. These proteins were mainly involved in sperm maturation, sperm capacitation, and sperm-egg interaction, suggesting that GP treatment was able to restore the fertility of male diabetic rats at molecular protein level. In conclusion, GP treatment effectively treats infertility of male diabetic rats, possibly through the upregulation of proteins related to sperm maturation and sperm-egg interaction. |
url |
http://dx.doi.org/10.1155/2018/9201539 |
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