Genetic and Antigenic Analysis of Adenovirus Type 3 Strains Showing Intermediate Behavior in Standard Seroneutralization Test
During an epidemiological survey of acute respiratory infection in Rio de Janeiro, among 208 adenovirus isolates, we found two strains that we were not able, by a standard neutralization procedure, to distinguish between type 3 or 7. However, DNA restriction pattern for the two strains with differen...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Instituto Oswaldo Cruz, Ministério da Saúde
1998-03-01
|
Series: | Memórias do Instituto Oswaldo Cruz. |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000200019 |
id |
doaj-1e863c253e6b44ccbc3836d3efb32c90 |
---|---|
record_format |
Article |
spelling |
doaj-1e863c253e6b44ccbc3836d3efb32c902020-11-24T21:06:16ZengInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz.0074-02761678-80601998-03-0193223110.1590/S0074-02761998000200019Genetic and Antigenic Analysis of Adenovirus Type 3 Strains Showing Intermediate Behavior in Standard Seroneutralization TestMárcia TB MoraesMessias da SilvaJosé Paulo G LeiteJussara P NascimentoDuring an epidemiological survey of acute respiratory infection in Rio de Janeiro, among 208 adenovirus isolates, we found two strains that we were not able, by a standard neutralization procedure, to distinguish between type 3 or 7. However, DNA restriction pattern for the two strains with different enzymes were analyzed and showed a typical Ad3h profile. Using a cross-neutralization test in which both Ad3p and Ad7p antisera were used in different concentration against 100 TCID50 of each adenovirus standard and both isolates, we were able to confirm that the two isolates belong to serotype 3. An hemagglutination inhibition test also corroborated the identification of both strains as adenovirus type 3. Comparing Ad3h and Ad3p genome, we observed 16 different restriction enzyme sites, three of which were located in genomic regions encoding polypeptides involved in neutralization siteshttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000200019adenovirus type 3genetic and antigenic analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Márcia TB Moraes Messias da Silva José Paulo G Leite Jussara P Nascimento |
spellingShingle |
Márcia TB Moraes Messias da Silva José Paulo G Leite Jussara P Nascimento Genetic and Antigenic Analysis of Adenovirus Type 3 Strains Showing Intermediate Behavior in Standard Seroneutralization Test Memórias do Instituto Oswaldo Cruz. adenovirus type 3 genetic and antigenic analysis |
author_facet |
Márcia TB Moraes Messias da Silva José Paulo G Leite Jussara P Nascimento |
author_sort |
Márcia TB Moraes |
title |
Genetic and Antigenic Analysis of Adenovirus Type 3 Strains Showing Intermediate Behavior in Standard Seroneutralization Test |
title_short |
Genetic and Antigenic Analysis of Adenovirus Type 3 Strains Showing Intermediate Behavior in Standard Seroneutralization Test |
title_full |
Genetic and Antigenic Analysis of Adenovirus Type 3 Strains Showing Intermediate Behavior in Standard Seroneutralization Test |
title_fullStr |
Genetic and Antigenic Analysis of Adenovirus Type 3 Strains Showing Intermediate Behavior in Standard Seroneutralization Test |
title_full_unstemmed |
Genetic and Antigenic Analysis of Adenovirus Type 3 Strains Showing Intermediate Behavior in Standard Seroneutralization Test |
title_sort |
genetic and antigenic analysis of adenovirus type 3 strains showing intermediate behavior in standard seroneutralization test |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
series |
Memórias do Instituto Oswaldo Cruz. |
issn |
0074-0276 1678-8060 |
publishDate |
1998-03-01 |
description |
During an epidemiological survey of acute respiratory infection in Rio de Janeiro, among 208 adenovirus isolates, we found two strains that we were not able, by a standard neutralization procedure, to distinguish between type 3 or 7. However, DNA restriction pattern for the two strains with different enzymes were analyzed and showed a typical Ad3h profile. Using a cross-neutralization test in which both Ad3p and Ad7p antisera were used in different concentration against 100 TCID50 of each adenovirus standard and both isolates, we were able to confirm that the two isolates belong to serotype 3. An hemagglutination inhibition test also corroborated the identification of both strains as adenovirus type 3. Comparing Ad3h and Ad3p genome, we observed 16 different restriction enzyme sites, three of which were located in genomic regions encoding polypeptides involved in neutralization sites |
topic |
adenovirus type 3 genetic and antigenic analysis |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000200019 |
work_keys_str_mv |
AT marciatbmoraes geneticandantigenicanalysisofadenovirustype3strainsshowingintermediatebehaviorinstandardseroneutralizationtest AT messiasdasilva geneticandantigenicanalysisofadenovirustype3strainsshowingintermediatebehaviorinstandardseroneutralizationtest AT josepaulogleite geneticandantigenicanalysisofadenovirustype3strainsshowingintermediatebehaviorinstandardseroneutralizationtest AT jussarapnascimento geneticandantigenicanalysisofadenovirustype3strainsshowingintermediatebehaviorinstandardseroneutralizationtest |
_version_ |
1716766105837502464 |