Screening potential mitochondria-targeting compounds from traditional Chinese medicines using a mitochondria-based centrifugal ultrafiltration/liquid chromatography/mass spectrometry method
Mitochondria regulate numerous crucial cell processes, including energy production, apoptotic cell death, oxidative stress, calcium homeostasis and lipid metabolism. Here, we applied an efficient mitochondria-based centrifugal ultrafiltration/liquid chromatography/mass spectrometry (LC/MS) method, a...
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doaj-1e91b3dbf0f944348355de066972c0c42021-04-02T06:46:38ZengElsevierJournal of Pharmaceutical Analysis2095-17792018-08-0184240249Screening potential mitochondria-targeting compounds from traditional Chinese medicines using a mitochondria-based centrifugal ultrafiltration/liquid chromatography/mass spectrometry methodXing-Xin Yang0Yu-Zhen Zhou1Feng Xu2Jie Yu3 Gegentana4Ming-Ying Shang5Xuan Wang6Shao-Qing Cai7Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, PR China; Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, PR China; College of Pharmaceutical Science, Yunnan University of Traditional Chinese Medicine, 1076 Yuhua Road, Kunming 650500, Yunnan Province, PR ChinaDepartment of Chemical Biology, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, PR ChinaDepartment of Natural Medicines, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, PR ChinaCollege of Pharmaceutical Science, Yunnan University of Traditional Chinese Medicine, 1076 Yuhua Road, Kunming 650500, Yunnan Province, PR ChinaDepartment of Natural Medicines, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, PR ChinaDepartment of Natural Medicines, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, PR ChinaDepartment of Chemical Biology, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, PR China; Corresponding authors.Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, PR China; Corresponding authors.Mitochondria regulate numerous crucial cell processes, including energy production, apoptotic cell death, oxidative stress, calcium homeostasis and lipid metabolism. Here, we applied an efficient mitochondria-based centrifugal ultrafiltration/liquid chromatography/mass spectrometry (LC/MS) method, also known as screening method for mitochondria-targeted bioactive constituents (SM-MBC). This method allowed searching natural mitochondria-targeting compounds from traditional Chinese medicines (TCMs), including Puerariae Radix (PR) and Chuanxiong Radix (CR). A total of 23 active compounds were successfully discovered from the two TCMs extracts. Among these 23 hit compounds, 17 were identified by LC/MS, 12 of which were novel mitochondria-targeting compounds. Among these, 6 active compounds were analyzed in vitro for pharmacological tests and found able to affect mitochondrial functions. We also investigated the effects of the hit compounds on HepG2 cell proliferation and on loss of cardiomyocyte viability induced by hypoxia/reoxygenation injury. The results obtained are useful for in-depth understanding of mechanisms underlying TCMs therapeutic effects at mitochondria level and for developing novel potential drugs using TCMs as lead compounds. Finally, we showed that SM-MBC was an efficient protocol for the rapid screening of mitochondria-targeting constituents from complex samples such as PR and CR extracts. Keywords: Mitochondria, Bioactive constituents, Traditional Chinese medicines, Centrifugal ultrafiltration, Liquid chromatography/mass spectrometryhttp://www.sciencedirect.com/science/article/pii/S2095177918300832 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xing-Xin Yang Yu-Zhen Zhou Feng Xu Jie Yu Gegentana Ming-Ying Shang Xuan Wang Shao-Qing Cai |
spellingShingle |
Xing-Xin Yang Yu-Zhen Zhou Feng Xu Jie Yu Gegentana Ming-Ying Shang Xuan Wang Shao-Qing Cai Screening potential mitochondria-targeting compounds from traditional Chinese medicines using a mitochondria-based centrifugal ultrafiltration/liquid chromatography/mass spectrometry method Journal of Pharmaceutical Analysis |
author_facet |
Xing-Xin Yang Yu-Zhen Zhou Feng Xu Jie Yu Gegentana Ming-Ying Shang Xuan Wang Shao-Qing Cai |
author_sort |
Xing-Xin Yang |
title |
Screening potential mitochondria-targeting compounds from traditional Chinese medicines using a mitochondria-based centrifugal ultrafiltration/liquid chromatography/mass spectrometry method |
title_short |
Screening potential mitochondria-targeting compounds from traditional Chinese medicines using a mitochondria-based centrifugal ultrafiltration/liquid chromatography/mass spectrometry method |
title_full |
Screening potential mitochondria-targeting compounds from traditional Chinese medicines using a mitochondria-based centrifugal ultrafiltration/liquid chromatography/mass spectrometry method |
title_fullStr |
Screening potential mitochondria-targeting compounds from traditional Chinese medicines using a mitochondria-based centrifugal ultrafiltration/liquid chromatography/mass spectrometry method |
title_full_unstemmed |
Screening potential mitochondria-targeting compounds from traditional Chinese medicines using a mitochondria-based centrifugal ultrafiltration/liquid chromatography/mass spectrometry method |
title_sort |
screening potential mitochondria-targeting compounds from traditional chinese medicines using a mitochondria-based centrifugal ultrafiltration/liquid chromatography/mass spectrometry method |
publisher |
Elsevier |
series |
Journal of Pharmaceutical Analysis |
issn |
2095-1779 |
publishDate |
2018-08-01 |
description |
Mitochondria regulate numerous crucial cell processes, including energy production, apoptotic cell death, oxidative stress, calcium homeostasis and lipid metabolism. Here, we applied an efficient mitochondria-based centrifugal ultrafiltration/liquid chromatography/mass spectrometry (LC/MS) method, also known as screening method for mitochondria-targeted bioactive constituents (SM-MBC). This method allowed searching natural mitochondria-targeting compounds from traditional Chinese medicines (TCMs), including Puerariae Radix (PR) and Chuanxiong Radix (CR). A total of 23 active compounds were successfully discovered from the two TCMs extracts. Among these 23 hit compounds, 17 were identified by LC/MS, 12 of which were novel mitochondria-targeting compounds. Among these, 6 active compounds were analyzed in vitro for pharmacological tests and found able to affect mitochondrial functions. We also investigated the effects of the hit compounds on HepG2 cell proliferation and on loss of cardiomyocyte viability induced by hypoxia/reoxygenation injury. The results obtained are useful for in-depth understanding of mechanisms underlying TCMs therapeutic effects at mitochondria level and for developing novel potential drugs using TCMs as lead compounds. Finally, we showed that SM-MBC was an efficient protocol for the rapid screening of mitochondria-targeting constituents from complex samples such as PR and CR extracts. Keywords: Mitochondria, Bioactive constituents, Traditional Chinese medicines, Centrifugal ultrafiltration, Liquid chromatography/mass spectrometry |
url |
http://www.sciencedirect.com/science/article/pii/S2095177918300832 |
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