Molecular epidemiology and disease severity of human respiratory syncytial virus in Vietnam.
Respiratory syncytial virus (RSV) is a major cause of acute respiratory infections (ARIs) in children worldwide and can cause high mortality, especially in developing countries. However, information on the clinical and molecular characteristics of RSV infection in developing countries is limited. Fr...
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doaj-1e9344516a884dd78cdf8af909a19ef32020-11-25T00:10:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0181e4543610.1371/journal.pone.0045436Molecular epidemiology and disease severity of human respiratory syncytial virus in Vietnam.Dinh Nguyen TranThi Minh Hong PhamManh Tuan HaThi Thu Loan TranThi Kim Huyen DangLay-Myint YoshidaShoko OkitsuSatoshi HayakawaMasashi MizuguchiHiroshi UshijimaRespiratory syncytial virus (RSV) is a major cause of acute respiratory infections (ARIs) in children worldwide and can cause high mortality, especially in developing countries. However, information on the clinical and molecular characteristics of RSV infection in developing countries is limited. From April 2010 to May 2011, 1,082 nasopharyngeal swabs were collected from children with ARI admitted to the Children's Hospital 2, Ho Chi Minh City, Vietnam. Samples were screened for RSV and genotyped by reverse transcription-PCR and sequencing. Demographic and clinical data was also recorded. RSV was found in 23.8% (257/1,082) of samples. RSV A was the dominant subgroup, accounting for 91.4% (235/257), followed by RSV B, 5.1% (13/257), and 9 cases (3.5%) were mixed infection of these subgroups. The phylogenetic analysis revealed that all group A strains belonged to the GA2 genotype. All group B strains belonged to the recently identified BA genotype, and further clustered into 2 recently described subgenotypes BA9 and BA10. One GA2 genotype strain had a premature stop codon which shortened the G protein length. RSV infection was significantly associated with younger age and higher severity score than those without. Co-infection with other viruses did not affect disease severity. RSV A caused more severe disease than RSV B. The results from this study will not only contribute to the growing database on the molecular diversity of RSV circulating worldwide but may be also useful in clinical management and vaccine development.http://europepmc.org/articles/PMC3551923?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dinh Nguyen Tran Thi Minh Hong Pham Manh Tuan Ha Thi Thu Loan Tran Thi Kim Huyen Dang Lay-Myint Yoshida Shoko Okitsu Satoshi Hayakawa Masashi Mizuguchi Hiroshi Ushijima |
spellingShingle |
Dinh Nguyen Tran Thi Minh Hong Pham Manh Tuan Ha Thi Thu Loan Tran Thi Kim Huyen Dang Lay-Myint Yoshida Shoko Okitsu Satoshi Hayakawa Masashi Mizuguchi Hiroshi Ushijima Molecular epidemiology and disease severity of human respiratory syncytial virus in Vietnam. PLoS ONE |
author_facet |
Dinh Nguyen Tran Thi Minh Hong Pham Manh Tuan Ha Thi Thu Loan Tran Thi Kim Huyen Dang Lay-Myint Yoshida Shoko Okitsu Satoshi Hayakawa Masashi Mizuguchi Hiroshi Ushijima |
author_sort |
Dinh Nguyen Tran |
title |
Molecular epidemiology and disease severity of human respiratory syncytial virus in Vietnam. |
title_short |
Molecular epidemiology and disease severity of human respiratory syncytial virus in Vietnam. |
title_full |
Molecular epidemiology and disease severity of human respiratory syncytial virus in Vietnam. |
title_fullStr |
Molecular epidemiology and disease severity of human respiratory syncytial virus in Vietnam. |
title_full_unstemmed |
Molecular epidemiology and disease severity of human respiratory syncytial virus in Vietnam. |
title_sort |
molecular epidemiology and disease severity of human respiratory syncytial virus in vietnam. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
Respiratory syncytial virus (RSV) is a major cause of acute respiratory infections (ARIs) in children worldwide and can cause high mortality, especially in developing countries. However, information on the clinical and molecular characteristics of RSV infection in developing countries is limited. From April 2010 to May 2011, 1,082 nasopharyngeal swabs were collected from children with ARI admitted to the Children's Hospital 2, Ho Chi Minh City, Vietnam. Samples were screened for RSV and genotyped by reverse transcription-PCR and sequencing. Demographic and clinical data was also recorded. RSV was found in 23.8% (257/1,082) of samples. RSV A was the dominant subgroup, accounting for 91.4% (235/257), followed by RSV B, 5.1% (13/257), and 9 cases (3.5%) were mixed infection of these subgroups. The phylogenetic analysis revealed that all group A strains belonged to the GA2 genotype. All group B strains belonged to the recently identified BA genotype, and further clustered into 2 recently described subgenotypes BA9 and BA10. One GA2 genotype strain had a premature stop codon which shortened the G protein length. RSV infection was significantly associated with younger age and higher severity score than those without. Co-infection with other viruses did not affect disease severity. RSV A caused more severe disease than RSV B. The results from this study will not only contribute to the growing database on the molecular diversity of RSV circulating worldwide but may be also useful in clinical management and vaccine development. |
url |
http://europepmc.org/articles/PMC3551923?pdf=render |
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