Histamine N-methyltransferase Modulates Human Bronchial Smooth Muscle Contraction
To elucidate the modulatory role of histamine-degrading enzymes in airway constrictor responses, human bronchial strips were studied under isometric conditions in vitro. Pretreatment of tissues with the histamine N-methyltransferase (HMT) inhibitor SKF 91488 specifically potentiated the contractile...
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1994-01-01
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Series: | Mediators of Inflammation |
Online Access: | http://dx.doi.org/10.1155/S0962935194000153 |
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doaj-1e966ef7267e477fb31d048c1aaebbef2020-11-24T23:41:33ZengHindawi LimitedMediators of Inflammation0962-93511466-18611994-01-013212512910.1155/S0962935194000153Histamine N-methyltransferase Modulates Human Bronchial Smooth Muscle ContractionJ. Tamaoki0A. Chiyotani1E. Tagaya2K. Isono3K. Konno4First Department of Medicine, Tokyo Women's Medical College, Tokyo 162, JapanFirst Department of Medicine, Tokyo Women's Medical College, Tokyo 162, JapanFirst Department of Medicine, Tokyo Women's Medical College, Tokyo 162, JapanFirst Department of Medicine, Tokyo Women's Medical College, Tokyo 162, JapanFirst Department of Medicine, Tokyo Women's Medical College, Tokyo 162, JapanTo elucidate the modulatory role of histamine-degrading enzymes in airway constrictor responses, human bronchial strips were studied under isometric conditions in vitro. Pretreatment of tissues with the histamine N-methyltransferase (HMT) inhibitor SKF 91488 specifically potentiated the contractile responses to histamine, causing a leftward displacement of the concentration response curves, whereas the diamine oxidase inhibitor aminoguanidine had no effect. This potentiation was attenuated by mechanical removal of the epithelium. The HMT activity was detected in the human bronchi, which was less in the epithelium-denuded tissues than the epithelium-intact tissues. These results suggest that HMT localized to the airway epithelium may play a protective role against histamine-mediated bronchoconstriction in humans.http://dx.doi.org/10.1155/S0962935194000153 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
J. Tamaoki A. Chiyotani E. Tagaya K. Isono K. Konno |
spellingShingle |
J. Tamaoki A. Chiyotani E. Tagaya K. Isono K. Konno Histamine N-methyltransferase Modulates Human Bronchial Smooth Muscle Contraction Mediators of Inflammation |
author_facet |
J. Tamaoki A. Chiyotani E. Tagaya K. Isono K. Konno |
author_sort |
J. Tamaoki |
title |
Histamine N-methyltransferase Modulates Human Bronchial Smooth Muscle Contraction |
title_short |
Histamine N-methyltransferase Modulates Human Bronchial Smooth Muscle Contraction |
title_full |
Histamine N-methyltransferase Modulates Human Bronchial Smooth Muscle Contraction |
title_fullStr |
Histamine N-methyltransferase Modulates Human Bronchial Smooth Muscle Contraction |
title_full_unstemmed |
Histamine N-methyltransferase Modulates Human Bronchial Smooth Muscle Contraction |
title_sort |
histamine n-methyltransferase modulates human bronchial smooth muscle contraction |
publisher |
Hindawi Limited |
series |
Mediators of Inflammation |
issn |
0962-9351 1466-1861 |
publishDate |
1994-01-01 |
description |
To elucidate the modulatory role of histamine-degrading enzymes in
airway constrictor responses, human bronchial strips were studied
under isometric conditions in vitro. Pretreatment of tissues with
the histamine N-methyltransferase (HMT) inhibitor SKF 91488
specifically potentiated the contractile responses to histamine,
causing a leftward displacement of the concentration response curves,
whereas the diamine oxidase inhibitor aminoguanidine had no effect.
This potentiation was attenuated by mechanical removal of the
epithelium. The HMT activity was detected in the human bronchi,
which was less in the epithelium-denuded tissues than the
epithelium-intact tissues. These results suggest that HMT localized
to the airway epithelium may play a protective role against
histamine-mediated bronchoconstriction in humans. |
url |
http://dx.doi.org/10.1155/S0962935194000153 |
work_keys_str_mv |
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