Effect of Cyclic Stretch on Vascular Endothelial Cells and Abdominal Aortic Aneurysm (AAA): Role in the Inflammatory Response
Abdominal aortic aneurysm (AAA) is a focal dilatation of the aorta, caused by both genetic and environmental factors. Although vascular endothelium plays a key role in AAA progression, the biological mechanisms underlying the mechanical stress involvement are only partially understood. In this study...
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doaj-1eb00523dd154767af4e1ae0d27071232020-11-25T01:25:00ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-01-0120228710.3390/ijms20020287ijms20020287Effect of Cyclic Stretch on Vascular Endothelial Cells and Abdominal Aortic Aneurysm (AAA): Role in the Inflammatory ResponseMartina Ramella0Giulia Bertozzi1Luca Fusaro2Maria Talmon3Marcello Manfredi4Marta Calvo Catoria5Francesco Casella6Carla Maria Porta7Renzo Boldorini8Luigia Grazia Fresu9Emilio Marengo10Francesca Boccafoschi11Department of Health Science, University of Piemonte Orientale (UPO), via Solaroli 17, 28100 Novara, ItalyDepartment of Health Science, University of Piemonte Orientale (UPO), via Solaroli 17, 28100 Novara, ItalyDepartment of Health Science, University of Piemonte Orientale (UPO), via Solaroli 17, 28100 Novara, ItalyDepartment of Health Science, University of Piemonte Orientale (UPO), via Solaroli 17, 28100 Novara, ItalyISALIT s.r.l., Spin-off of DISIT, University of Piemonte Orientale (UPO), 15121 Alessandria, ItalyDepartment of Health Science, University of Piemonte Orientale (UPO), via Solaroli 17, 28100 Novara, ItalyVascular Surgery Unit, Ospedale Maggiore della Carità, 28100 Novara, ItalyVascular Surgery Unit, Ospedale Maggiore della Carità, 28100 Novara, ItalyDepartment of Health Science, University of Piemonte Orientale (UPO), via Solaroli 17, 28100 Novara, ItalyDepartment of Health Science, University of Piemonte Orientale (UPO), via Solaroli 17, 28100 Novara, ItalyDepartment of Sciences and Technological Innovation, University of Piemonte Orientale (UPO), 15121 Alessandria, ItalyDepartment of Health Science, University of Piemonte Orientale (UPO), via Solaroli 17, 28100 Novara, ItalyAbdominal aortic aneurysm (AAA) is a focal dilatation of the aorta, caused by both genetic and environmental factors. Although vascular endothelium plays a key role in AAA progression, the biological mechanisms underlying the mechanical stress involvement are only partially understood. In this study, we developed an in vitro model to characterize the role of mechanical stress as a potential trigger of endothelial deregulation in terms of inflammatory response bridging between endothelial cells (ECs), inflammatory cells, and matrix remodeling. In AAA patients, data revealed different degrees of calcification, inversely correlated with wall stretching and also with inflammation and extracellular matrix degradation. In order to study the role of mechanical stimulation, endothelial cell line (EA.hy926) has been cultured in healthy (10% strain) and pathological (5% strain) dynamic conditions using a bioreactor. In presence of tumor necrosis factor alpha (TNF-α), high levels of matrix metalloproteinase-9 (MMP-9) expression and inflammation are obtained, while mechanical stimulation significantly counteracts the TNF-α effects. Moreover, physiological deformation also plays a significant role in the control of the oxidative stress. Overall our findings indicate that, due to wall calcification, in AAA there is a significant change in terms of decreased wall stretching.http://www.mdpi.com/1422-0067/20/2/287cardiovascular diseasesabdominal aortic aneurysmoxidative stressinflammationcalcificationcyclic stretch |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Martina Ramella Giulia Bertozzi Luca Fusaro Maria Talmon Marcello Manfredi Marta Calvo Catoria Francesco Casella Carla Maria Porta Renzo Boldorini Luigia Grazia Fresu Emilio Marengo Francesca Boccafoschi |
spellingShingle |
Martina Ramella Giulia Bertozzi Luca Fusaro Maria Talmon Marcello Manfredi Marta Calvo Catoria Francesco Casella Carla Maria Porta Renzo Boldorini Luigia Grazia Fresu Emilio Marengo Francesca Boccafoschi Effect of Cyclic Stretch on Vascular Endothelial Cells and Abdominal Aortic Aneurysm (AAA): Role in the Inflammatory Response International Journal of Molecular Sciences cardiovascular diseases abdominal aortic aneurysm oxidative stress inflammation calcification cyclic stretch |
author_facet |
Martina Ramella Giulia Bertozzi Luca Fusaro Maria Talmon Marcello Manfredi Marta Calvo Catoria Francesco Casella Carla Maria Porta Renzo Boldorini Luigia Grazia Fresu Emilio Marengo Francesca Boccafoschi |
author_sort |
Martina Ramella |
title |
Effect of Cyclic Stretch on Vascular Endothelial Cells and Abdominal Aortic Aneurysm (AAA): Role in the Inflammatory Response |
title_short |
Effect of Cyclic Stretch on Vascular Endothelial Cells and Abdominal Aortic Aneurysm (AAA): Role in the Inflammatory Response |
title_full |
Effect of Cyclic Stretch on Vascular Endothelial Cells and Abdominal Aortic Aneurysm (AAA): Role in the Inflammatory Response |
title_fullStr |
Effect of Cyclic Stretch on Vascular Endothelial Cells and Abdominal Aortic Aneurysm (AAA): Role in the Inflammatory Response |
title_full_unstemmed |
Effect of Cyclic Stretch on Vascular Endothelial Cells and Abdominal Aortic Aneurysm (AAA): Role in the Inflammatory Response |
title_sort |
effect of cyclic stretch on vascular endothelial cells and abdominal aortic aneurysm (aaa): role in the inflammatory response |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2019-01-01 |
description |
Abdominal aortic aneurysm (AAA) is a focal dilatation of the aorta, caused by both genetic and environmental factors. Although vascular endothelium plays a key role in AAA progression, the biological mechanisms underlying the mechanical stress involvement are only partially understood. In this study, we developed an in vitro model to characterize the role of mechanical stress as a potential trigger of endothelial deregulation in terms of inflammatory response bridging between endothelial cells (ECs), inflammatory cells, and matrix remodeling. In AAA patients, data revealed different degrees of calcification, inversely correlated with wall stretching and also with inflammation and extracellular matrix degradation. In order to study the role of mechanical stimulation, endothelial cell line (EA.hy926) has been cultured in healthy (10% strain) and pathological (5% strain) dynamic conditions using a bioreactor. In presence of tumor necrosis factor alpha (TNF-α), high levels of matrix metalloproteinase-9 (MMP-9) expression and inflammation are obtained, while mechanical stimulation significantly counteracts the TNF-α effects. Moreover, physiological deformation also plays a significant role in the control of the oxidative stress. Overall our findings indicate that, due to wall calcification, in AAA there is a significant change in terms of decreased wall stretching. |
topic |
cardiovascular diseases abdominal aortic aneurysm oxidative stress inflammation calcification cyclic stretch |
url |
http://www.mdpi.com/1422-0067/20/2/287 |
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