A switchable light-input, light-output system modelled and constructed in yeast

• Main Authors: Kozma-Bognar Laszlo, Dixon Laura E, Terecskei Kata, Kapus Anita, Sorokina Oxana, Nagy Ferenc, Millar Andrew J
• Format: Article
• Language: English
• Published: BMC 2009-09-01
• Series: Journal of Biological Engineering
• Online Access: http://www.jbioleng.org/content/3/1/15

<p>Abstract</p> <p>Background</p> <p>Advances in synthetic biology will require spatio-temporal regulation of biological processes in heterologous host cells. We develop a light-switchable, two-hybrid interaction in yeast, based upon the Arabidopsis proteins PHYTOCHROME A and FAR-RED ELONGATED HYPOCOTYL 1-LIKE. Light input to this regulatory module allows dynamic control of a light-emitting LUCIFERASE reporter gene, which we detect by real-time imaging of yeast colonies on solid media.</p> <p>Results</p> <p>The reversible activation of the phytochrome by red light, and its inactivation by far-red light, is retained. We use this quantitative readout to construct a mathematical model that matches the system's behaviour and predicts the molecular targets for future manipulation.</p> <p>Conclusion</p> <p>Our model, methods and materials together constitute a novel system for a eukaryotic host with the potential to convert a dynamic pattern of light input into a predictable gene expression response. This system could be applied for the regulation of genetic networks - both known and synthetic.</p>