Relationship between apoptosis and the BH2 domain sequence of the VP5 peptide of infectious pancreatic necrosis virus
Objective. To determine whether the level of apoptosis induced by infectious pancreatic necrosis virus (IPNV) is related to the amino acid sequence of the BH2 domain of the VP5 protein and the level of infectivity. Materials and methods. Three IPNV strains were used, the VP2 protein gene was amplifi...
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2014-03-01
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doaj-1effcb236716409f9d290888d3f3fffd2020-11-24T20:56:12ZengUniversidad de CordobaRevista MVZ Cordoba0122-02681909-05442014-03-0119139904002Relationship between apoptosis and the BH2 domain sequence of the VP5 peptide of infectious pancreatic necrosis virusCesar Ortega S.0Sylvia Rodríguez S.1Juan Carlos Espinoza2Juan Kuznar3Alex Romero4Ricardo Enríquez5Universidad Autonoma del Estado de México, Faculty of Veterinary Medicine and Animal Husbandry, Advanced Animal Health Research and Study Center. Toluca, Mexico, AP. 4-56Biologic Research Center (High Council for Scientific Research) c/ Ramiro de Maeztu 9 Madrid 28040, Spain.Universidad de Valparaiso, Faculty of Sciences, Biochemistry and Virology Laboratory. Gran Bretaña 1111, Valparaíso, Chile.Universidad de Valparaiso, Faculty of Sciences, Biochemistry and Virology Laboratory. Gran Bretaña 1111, Valparaíso, Chile.Centro FONDAP: Interdisciplinary Center for Aquaculture Research (INCAR), Chile.Universidad Austral de Chile, Faculty of Veterinary Sciences, Biotechnology and Aquatic Pathology Laboratory. Valdivia, Chile.Objective. To determine whether the level of apoptosis induced by infectious pancreatic necrosis virus (IPNV) is related to the amino acid sequence of the BH2 domain of the VP5 protein and the level of infectivity. Materials and methods. Three IPNV strains were used, the VP2 protein gene was amplified for genotyping and the VP5 sequence was also obtained. The infectivity of the strains was calculated using the viral titer obtained at 12, 24, 36 and 45 hpi in CHSE-214 cells. The percentage of apoptosis in infected cells was visualized by TUNEL assay and immunohistochemistry (caspase 3 detection). Results. The V70/06 and V33/98 strains corresponded to genotype Sp, while V112/06 to VR-299; the amino acid analysis of the V70/06 strain allows its classification as middle virulent strain and V33/98 and V112/06 strains as low virulent ones; infection with the V112/06 strain produced a lower viral titer (p<0.05). The VP5 gene of the 3 strains showed four homologous domains to Bcl-2, however, the BH2 domain was truncated in V70/06 and V33/98 (12 kDa), being complete (15kDa) in V112/06, which also showed the Trp155 residue, equivalent to Trp188 considered as a critical factor for the function of Bcl-2. The average apoptosis was below 12%, showing no differences between strains (p>0.05). Conclusions. The results showed that the differences in the BH2 sequence of the VP5 protein, infectivity and the VP2 sequence are not associated with the modulation of apoptosis.http://apps.unicordoba.edu.co/revistas/revistamvz/mvz-191/v19n1a09.pdfApoptosisInfectivityIPNVVP5 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cesar Ortega S. Sylvia Rodríguez S. Juan Carlos Espinoza Juan Kuznar Alex Romero Ricardo Enríquez |
spellingShingle |
Cesar Ortega S. Sylvia Rodríguez S. Juan Carlos Espinoza Juan Kuznar Alex Romero Ricardo Enríquez Relationship between apoptosis and the BH2 domain sequence of the VP5 peptide of infectious pancreatic necrosis virus Revista MVZ Cordoba Apoptosis Infectivity IPNV VP5 |
author_facet |
Cesar Ortega S. Sylvia Rodríguez S. Juan Carlos Espinoza Juan Kuznar Alex Romero Ricardo Enríquez |
author_sort |
Cesar Ortega S. |
title |
Relationship between apoptosis and the BH2 domain sequence of the VP5 peptide of infectious pancreatic necrosis virus |
title_short |
Relationship between apoptosis and the BH2 domain sequence of the VP5 peptide of infectious pancreatic necrosis virus |
title_full |
Relationship between apoptosis and the BH2 domain sequence of the VP5 peptide of infectious pancreatic necrosis virus |
title_fullStr |
Relationship between apoptosis and the BH2 domain sequence of the VP5 peptide of infectious pancreatic necrosis virus |
title_full_unstemmed |
Relationship between apoptosis and the BH2 domain sequence of the VP5 peptide of infectious pancreatic necrosis virus |
title_sort |
relationship between apoptosis and the bh2 domain sequence of the vp5 peptide of infectious pancreatic necrosis virus |
publisher |
Universidad de Cordoba |
series |
Revista MVZ Cordoba |
issn |
0122-0268 1909-0544 |
publishDate |
2014-03-01 |
description |
Objective. To determine whether the level of apoptosis induced by infectious pancreatic necrosis virus (IPNV) is related to the amino acid sequence of the BH2 domain of the VP5 protein and the level of infectivity. Materials and methods. Three IPNV strains were used, the VP2 protein gene was amplified for genotyping and the VP5 sequence was also obtained. The infectivity of the strains was calculated using the viral titer obtained at 12, 24, 36 and 45 hpi in CHSE-214 cells. The percentage of apoptosis in infected cells was visualized by TUNEL assay and immunohistochemistry (caspase 3 detection). Results. The V70/06 and V33/98 strains corresponded to genotype Sp, while V112/06 to VR-299; the amino acid analysis of the V70/06 strain allows its classification as middle virulent strain and V33/98 and V112/06 strains as low virulent ones; infection with the V112/06 strain produced a lower viral titer (p<0.05). The VP5 gene of the 3 strains showed four homologous domains to Bcl-2, however, the BH2 domain was truncated in V70/06 and V33/98 (12 kDa), being complete (15kDa) in V112/06, which also showed the Trp155 residue, equivalent to Trp188 considered as a critical factor for the function of Bcl-2. The average apoptosis was below 12%, showing no differences between strains (p>0.05). Conclusions. The results showed that the differences in the BH2 sequence of the VP5 protein, infectivity and the VP2 sequence are not associated with the modulation of apoptosis. |
topic |
Apoptosis Infectivity IPNV VP5 |
url |
http://apps.unicordoba.edu.co/revistas/revistamvz/mvz-191/v19n1a09.pdf |
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