Bacteria with Different Assemblages in the Soil Profile Drive the Diverse Nutrient Cycles in the Sugarcane Straw Retention Ecosystem

• Main Authors: Caifang Zhang, Muhammad Tayyab, Ahmad Yusuf Abubakar, Ziqi Yang, Ziqin Pang, Waqar Islam, Zhaoli Lin, Shiyan Li, Jun Luo, Xiaoliang Fan, Nyumah Fallah, Hua Zhang
• Format: Article
• Language: English
• Published: MDPI AG 2019-10-01
• Series: Diversity
• Subjects: soil profile; sugarcane straw retention; soil enzymes; soil fertility; 16s rrna gene amplicon; bacterial communities
• Online Access: https://www.mdpi.com/1424-2818/11/10/194

Straw retention, an alternative to artificial fertilization, commonly mitigates soil degradation and positively affects soil fertility. In this study, we investigated the succession of soil bacteria during two sugarcane straw retention treatments (control (CK) and sugarcane straw retention (SR)) and at four depths (0&#8722;10, 10&#8722;20, 20&#8722;30, and 30&#8722;40 cm) in fallow soil in a sugarcane cropping system. Using an Illumina MiSeq (16S rRNA) and soil enzyme activity, we explored the SR influence on soil bacterial communities and enzyme activities and its inclusive impact on soil fertility, with an emphasis on topsoil (0&#8722;10 cm) and subsoil (10&#8722;40 cm). Our results show that SR effectively improved soil fertility indicators (C, N, and P), including enzyme activities (C and N cycling), throughout the soil profile: these soil parameters greatly improved in the topsoil compared to the control. Sugarcane straw retention and soil depth (0&#8722;10 cm vs. 10&#8722;40 cm) were associated with little variation in bacterial species richness and alpha diversity throughout the soil profile. Subsoil and topsoil bacterial communities differed in composition. Compared to the CK treatment, SR enriched the topsoil with <i>Proteobacteria</i>, <i>Verrucomicrobia</i>, <i>Actinobacteria</i>, <i>Chloroflexi</i>, and <i>Nitrospirae</i>, while the subsoil was depleted in <i>Nitrospirae</i> and <i>Acidobacteria</i>. Similarly, SR enriched the subsoil with <i>Proteobacteria</i>, <i>Verrucomicrobia</i>, <i>Actinobacteria</i>, <i>Chloroflexi</i>, <i>Gemmatimonadetes</i>, and <i>Bacteroidetes</i>, while the topsoil was depleted in <i>Acidobacteria</i>, <i>Gemmatimonadetes</i>, and <i>Planctomycetes</i> compared to the CK. At the genus level, SR enriched the topsoil with <i>Gp1</i>, <i>Gp2</i>, <i>Gp5</i>, <i>Gp7</i>, <i>Gemmatimonas</i>, <i>Kofleria</i>, <i>Sphingomonas</i>, and <i>Gaiella</i>, which decompose lignocellulose and contribute to nutrient cycling. In summary, SR not only improved soil physicochemical properties and enzyme activities but also enriched bacterial taxa involved in lignocellulosic decomposition and nutrient cycling (C and N) throughout the soil profile. However, these effects were stronger in topsoil than in subsoil, suggesting that SR enhanced fertility more in topsoil than in subsoil in fallow land.