Quantification of sterol lipids in plants by quadrupole time-of-flight mass spectrometry
Glycerolipids, sphingolipids, and sterol lipids constitute the major lipid classes in plants. Sterol lipids are composed of free and conjugated sterols, i.e., sterol esters, sterol glycosides, and acylated sterol glycosides. Sterol lipids play crucial roles during adaption to abiotic stresses and pl...
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doaj-1f0bce81602a46728e9e5d493385fc112021-04-28T06:03:33ZengElsevierJournal of Lipid Research0022-22752011-05-0152510391054Quantification of sterol lipids in plants by quadrupole time-of-flight mass spectrometryVera Wewer0Isabel Dombrink1Katharina vom Dorp2Peter Dörmann3Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, 53115 Bonn, GermanyInstitute of Molecular Physiology and Biotechnology of Plants, University of Bonn, 53115 Bonn, GermanyInstitute of Molecular Physiology and Biotechnology of Plants, University of Bonn, 53115 Bonn, GermanyTo whom correspondence should be addressed. doermann@uni-bonn.de; To whom correspondence should be addressed. doermann@uni-bonn.de; Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, 53115 Bonn, GermanyGlycerolipids, sphingolipids, and sterol lipids constitute the major lipid classes in plants. Sterol lipids are composed of free and conjugated sterols, i.e., sterol esters, sterol glycosides, and acylated sterol glycosides. Sterol lipids play crucial roles during adaption to abiotic stresses and plant-pathogen interactions. Presently, no comprehensive method for sterol lipid quantification in plants is available. We used nanospray ionization quadrupole-time-of-flight mass spectrometry (Q-TOF MS) to resolve and identify the molecular species of all four sterol lipid classes from Arabidopsis thaliana. Free sterols were derivatized with chlorobetainyl chloride. Sterol esters, sterol glycosides, and acylated sterol glycosides were ionized as ammonium adducts. Quantification of molecular species was achieved in the positive mode after fragmentation in the presence of internal standards. The amounts of sterol lipids quantified by Q-TOF MS/MS were validated by comparison with results obtained with TLC/GC. Quantification of sterol lipids from leaves and roots of phosphate-deprived A. thaliana plants revealed changes in the amounts and molecular species composition. The Q-TOF method is far more sensitive than GC or HPLC. Therefore, Q-TOF MS/MS provides a comprehensive strategy for sterol lipid quantification that can be adapted to other tandem mass spectrometers.http://www.sciencedirect.com/science/article/pii/S0022227520408909Arabidopsiscampesterolcholesterolphosphate limitationphytosterolsitosterol |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Vera Wewer Isabel Dombrink Katharina vom Dorp Peter Dörmann |
spellingShingle |
Vera Wewer Isabel Dombrink Katharina vom Dorp Peter Dörmann Quantification of sterol lipids in plants by quadrupole time-of-flight mass spectrometry Journal of Lipid Research Arabidopsis campesterol cholesterol phosphate limitation phytosterol sitosterol |
author_facet |
Vera Wewer Isabel Dombrink Katharina vom Dorp Peter Dörmann |
author_sort |
Vera Wewer |
title |
Quantification of sterol lipids in plants by quadrupole time-of-flight mass spectrometry |
title_short |
Quantification of sterol lipids in plants by quadrupole time-of-flight mass spectrometry |
title_full |
Quantification of sterol lipids in plants by quadrupole time-of-flight mass spectrometry |
title_fullStr |
Quantification of sterol lipids in plants by quadrupole time-of-flight mass spectrometry |
title_full_unstemmed |
Quantification of sterol lipids in plants by quadrupole time-of-flight mass spectrometry |
title_sort |
quantification of sterol lipids in plants by quadrupole time-of-flight mass spectrometry |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
2011-05-01 |
description |
Glycerolipids, sphingolipids, and sterol lipids constitute the major lipid classes in plants. Sterol lipids are composed of free and conjugated sterols, i.e., sterol esters, sterol glycosides, and acylated sterol glycosides. Sterol lipids play crucial roles during adaption to abiotic stresses and plant-pathogen interactions. Presently, no comprehensive method for sterol lipid quantification in plants is available. We used nanospray ionization quadrupole-time-of-flight mass spectrometry (Q-TOF MS) to resolve and identify the molecular species of all four sterol lipid classes from Arabidopsis thaliana. Free sterols were derivatized with chlorobetainyl chloride. Sterol esters, sterol glycosides, and acylated sterol glycosides were ionized as ammonium adducts. Quantification of molecular species was achieved in the positive mode after fragmentation in the presence of internal standards. The amounts of sterol lipids quantified by Q-TOF MS/MS were validated by comparison with results obtained with TLC/GC. Quantification of sterol lipids from leaves and roots of phosphate-deprived A. thaliana plants revealed changes in the amounts and molecular species composition. The Q-TOF method is far more sensitive than GC or HPLC. Therefore, Q-TOF MS/MS provides a comprehensive strategy for sterol lipid quantification that can be adapted to other tandem mass spectrometers. |
topic |
Arabidopsis campesterol cholesterol phosphate limitation phytosterol sitosterol |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520408909 |
work_keys_str_mv |
AT verawewer quantificationofsterollipidsinplantsbyquadrupoletimeofflightmassspectrometry AT isabeldombrink quantificationofsterollipidsinplantsbyquadrupoletimeofflightmassspectrometry AT katharinavomdorp quantificationofsterollipidsinplantsbyquadrupoletimeofflightmassspectrometry AT peterdormann quantificationofsterollipidsinplantsbyquadrupoletimeofflightmassspectrometry |
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1721504312472895488 |