Synthesis and evaluation of radiolabeled, folic acid-PEG conjugated, amino silane coated magnetic nanoparticles in tumor bearing Balb/C mice

To design a potent agent for positron emission tomography/magnetic resonance imaging (PET/MRI) imaging and targeted magnetic hyperthermia-radioisotope cancer therapy radiolabeled surface modified superparamagnetic iron oxide nanoparticles (SPIONs) were used as nanocarriers. Folic acid was conjugated...

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Main Authors: Razjouyan Javad, Zolata Hamidreza, Khayat Omid, Nowshiravan Fereidoun, Shadanpour Nami, Mohammadnia Meisam
Format: Article
Language:English
Published: Sciendo 2015-07-01
Series:Nukleonika
Subjects:
Online Access:https://doi.org/10.1515/nuka-2015-0066
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spelling doaj-1f0d29290fac4fec9b976756ca3f49152021-09-06T19:21:14ZengSciendoNukleonika0029-59222015-07-0160349750210.1515/nuka-2015-0066nuka-2015-0066Synthesis and evaluation of radiolabeled, folic acid-PEG conjugated, amino silane coated magnetic nanoparticles in tumor bearing Balb/C miceRazjouyan Javad0Zolata Hamidreza1Khayat Omid2Nowshiravan Fereidoun3Shadanpour Nami4Mohammadnia Meisam5Department of Engineering, Garmsar Branch, Islamic Azad University, Garmsar, Iran, Tel.: +9891 0942 2335, Fax: 026 3446 4053Department of Energy Engineering and Physics, Amirkabir University of Technology, Tehran, IranYoung Researchers and Elite Club, South Tehran Branch, Islamic Azad University, Tehran, IranBiomedical Engineering Department, Science and Research Branch, Islamic Azad University, Tehran, IranNuclear Medicine Research Group, Alborz Research Center, Karaj, IranNuclear Engineering Department, East Tehran Branch, Islamic Azad University, Tehran, IranTo design a potent agent for positron emission tomography/magnetic resonance imaging (PET/MRI) imaging and targeted magnetic hyperthermia-radioisotope cancer therapy radiolabeled surface modified superparamagnetic iron oxide nanoparticles (SPIONs) were used as nanocarriers. Folic acid was conjugated for increasing selective cellular binding and internalization through receptor-mediated endocytosis. SPIONs were synthesized by the thermal decomposition of tris (acetylacetonato) iron (III) to achieve narrow and uniform nanoparticles. To increase the biocompatibility of SPIONs, they were coated with (3-aminopropyl) triethoxysilane (APTES), and then conjugated with synthesized folic acid-polyethylene glycol (FA-PEG) through amine group of (3-aminopropyl) triethoxysilane. Finally, the particles were labeled with 64Cu (t1/2 = 12.7 h) using 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid mono (N-hydroxy succinimide ester) DOTA-NHS chelator. After the characterization of SPIONs, their cellular internalization was evaluated in folate receptor (FR) overexpressing KB (established from a HeLa cell contamination) and mouse fibroblast cell (MFB) lines. Eventually, active and passive targeting effects of complex were assessed in KB tumor-bearing Balb/C mice through biodistribution studies. Synthesized bare SPIONs had low toxicity effect on healthy cells, but surface modification increased their biocompatibility. Moreover, KB cells viability was reduced when using folate conjugated SPIONs due to FR-mediated endocytosis, while having little effect on healthy cells (MFB). Moreover, this radiotracer had tolerable in vivo characteristics and tumor uptake. In the receptor blocked case, tumor uptake was decreased, indicating FR-specific uptake in tumor tissue while enhanced permeability and retention effect was major mechanism for tumor uptake.https://doi.org/10.1515/nuka-2015-0066superparamagnetic iron oxide nanoparticles (spions)(3-aminopropyl) triethoxysilane (aptes)poly ethylene glycol (peg)folic acidkb cellscopper-64
collection DOAJ
language English
format Article
sources DOAJ
author Razjouyan Javad
Zolata Hamidreza
Khayat Omid
Nowshiravan Fereidoun
Shadanpour Nami
Mohammadnia Meisam
spellingShingle Razjouyan Javad
Zolata Hamidreza
Khayat Omid
Nowshiravan Fereidoun
Shadanpour Nami
Mohammadnia Meisam
Synthesis and evaluation of radiolabeled, folic acid-PEG conjugated, amino silane coated magnetic nanoparticles in tumor bearing Balb/C mice
Nukleonika
superparamagnetic iron oxide nanoparticles (spions)
(3-aminopropyl) triethoxysilane (aptes)
poly ethylene glycol (peg)
folic acid
kb cells
copper-64
author_facet Razjouyan Javad
Zolata Hamidreza
Khayat Omid
Nowshiravan Fereidoun
Shadanpour Nami
Mohammadnia Meisam
author_sort Razjouyan Javad
title Synthesis and evaluation of radiolabeled, folic acid-PEG conjugated, amino silane coated magnetic nanoparticles in tumor bearing Balb/C mice
title_short Synthesis and evaluation of radiolabeled, folic acid-PEG conjugated, amino silane coated magnetic nanoparticles in tumor bearing Balb/C mice
title_full Synthesis and evaluation of radiolabeled, folic acid-PEG conjugated, amino silane coated magnetic nanoparticles in tumor bearing Balb/C mice
title_fullStr Synthesis and evaluation of radiolabeled, folic acid-PEG conjugated, amino silane coated magnetic nanoparticles in tumor bearing Balb/C mice
title_full_unstemmed Synthesis and evaluation of radiolabeled, folic acid-PEG conjugated, amino silane coated magnetic nanoparticles in tumor bearing Balb/C mice
title_sort synthesis and evaluation of radiolabeled, folic acid-peg conjugated, amino silane coated magnetic nanoparticles in tumor bearing balb/c mice
publisher Sciendo
series Nukleonika
issn 0029-5922
publishDate 2015-07-01
description To design a potent agent for positron emission tomography/magnetic resonance imaging (PET/MRI) imaging and targeted magnetic hyperthermia-radioisotope cancer therapy radiolabeled surface modified superparamagnetic iron oxide nanoparticles (SPIONs) were used as nanocarriers. Folic acid was conjugated for increasing selective cellular binding and internalization through receptor-mediated endocytosis. SPIONs were synthesized by the thermal decomposition of tris (acetylacetonato) iron (III) to achieve narrow and uniform nanoparticles. To increase the biocompatibility of SPIONs, they were coated with (3-aminopropyl) triethoxysilane (APTES), and then conjugated with synthesized folic acid-polyethylene glycol (FA-PEG) through amine group of (3-aminopropyl) triethoxysilane. Finally, the particles were labeled with 64Cu (t1/2 = 12.7 h) using 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid mono (N-hydroxy succinimide ester) DOTA-NHS chelator. After the characterization of SPIONs, their cellular internalization was evaluated in folate receptor (FR) overexpressing KB (established from a HeLa cell contamination) and mouse fibroblast cell (MFB) lines. Eventually, active and passive targeting effects of complex were assessed in KB tumor-bearing Balb/C mice through biodistribution studies. Synthesized bare SPIONs had low toxicity effect on healthy cells, but surface modification increased their biocompatibility. Moreover, KB cells viability was reduced when using folate conjugated SPIONs due to FR-mediated endocytosis, while having little effect on healthy cells (MFB). Moreover, this radiotracer had tolerable in vivo characteristics and tumor uptake. In the receptor blocked case, tumor uptake was decreased, indicating FR-specific uptake in tumor tissue while enhanced permeability and retention effect was major mechanism for tumor uptake.
topic superparamagnetic iron oxide nanoparticles (spions)
(3-aminopropyl) triethoxysilane (aptes)
poly ethylene glycol (peg)
folic acid
kb cells
copper-64
url https://doi.org/10.1515/nuka-2015-0066
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