Dual effect of neutrophils on secretory component production by human bronchial epithelial cells
A decreased bronchial expression of secretory component (SC) was demonstrated in severe COPD, and correlated with neutrophils. Mechanisms of epithelial cell/neutrophils interactions remain however poorly understood. Calu-3 (human bronchial epithelial) cells were incubated after confluence (in tripli...
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European Respiratory Society
2006-12-01
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doaj-1f194f77f05f4198998003f68a8ff67a2020-11-25T02:47:16ZengEuropean Respiratory SocietyEuropean Respiratory Review0905-91801600-06172006-12-0115101175176Dual effect of neutrophils on secretory component production by human bronchial epithelial cellsC. PiletteB. DetryA. GuissetY. SibilleA decreased bronchial expression of secretory component (SC) was demonstrated in severe COPD, and correlated with neutrophils. Mechanisms of epithelial cell/neutrophils interactions remain however poorly understood. Calu-3 (human bronchial epithelial) cells were incubated after confluence (in triplicate conditions) with various ratios of activated neutrophils (0.5:1 to 15:1, neutrophils: Calu-3 cells). After 48hrs of co-culture supernatants were assayed for SC by ELISA. SC production by Calu-3 cells increased at intermediate neutrophil numbers (316±32 versus 193±19ng·ml–1, ratio of 5:1 versus control, mean±SEM of 3 experiments, p = 0.05). In contrast, a trend for decrease in SC was observed with high neutrophil numbers (111±19 versus 193±19ng·ml–1, ratio of 15:1 versus control, p = 0.06). The addition of secretory leukocyte protease inhibitor further increased SC upregulation at intermediate ratios, and inhibited the SC decrease at high neutrophil numbers. The mechanism of SC up-regulation by neutrophils did not implicate TNF-alpha or IL-1beta. This study provides direct evidence of a dual effect of neutrophils on epithelial SC. Our data suggest that neutrophils could differently affect epithelial immune secretory function according to the extent of neutrophil influx and/or to the reactivity of airway epithelial cells. http://err.ersjournals.com/cgi/content/full/15/101/175 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
C. Pilette B. Detry A. Guisset Y. Sibille |
spellingShingle |
C. Pilette B. Detry A. Guisset Y. Sibille Dual effect of neutrophils on secretory component production by human bronchial epithelial cells European Respiratory Review |
author_facet |
C. Pilette B. Detry A. Guisset Y. Sibille |
author_sort |
C. Pilette |
title |
Dual effect of neutrophils on secretory component production by human bronchial epithelial cells |
title_short |
Dual effect of neutrophils on secretory component production by human bronchial epithelial cells |
title_full |
Dual effect of neutrophils on secretory component production by human bronchial epithelial cells |
title_fullStr |
Dual effect of neutrophils on secretory component production by human bronchial epithelial cells |
title_full_unstemmed |
Dual effect of neutrophils on secretory component production by human bronchial epithelial cells |
title_sort |
dual effect of neutrophils on secretory component production by human bronchial epithelial cells |
publisher |
European Respiratory Society |
series |
European Respiratory Review |
issn |
0905-9180 1600-0617 |
publishDate |
2006-12-01 |
description |
A decreased bronchial expression of secretory component (SC) was demonstrated in severe COPD, and correlated with neutrophils. Mechanisms of epithelial cell/neutrophils interactions remain however poorly understood. Calu-3 (human bronchial epithelial) cells were incubated after confluence (in triplicate conditions) with various ratios of activated neutrophils (0.5:1 to 15:1, neutrophils: Calu-3 cells). After 48hrs of co-culture supernatants were assayed for SC by ELISA. SC production by Calu-3 cells increased at intermediate neutrophil numbers (316±32 versus 193±19ng·ml–1, ratio of 5:1 versus control, mean±SEM of 3 experiments, p = 0.05). In contrast, a trend for decrease in SC was observed with high neutrophil numbers (111±19 versus 193±19ng·ml–1, ratio of 15:1 versus control, p = 0.06). The addition of secretory leukocyte protease inhibitor further increased SC upregulation at intermediate ratios, and inhibited the SC decrease at high neutrophil numbers. The mechanism of SC up-regulation by neutrophils did not implicate TNF-alpha or IL-1beta. This study provides direct evidence of a dual effect of neutrophils on epithelial SC. Our data suggest that neutrophils could differently affect epithelial immune secretory function according to the extent of neutrophil influx and/or to the reactivity of airway epithelial cells. |
url |
http://err.ersjournals.com/cgi/content/full/15/101/175 |
work_keys_str_mv |
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