A Human Gonadal Cell Model From Induced Pluripotent Stem Cells
Sertoli cells are main players in the male gonads development and their study may shed light on 46,XY disorders of sex development (DSD). Mature primary Sertoli cells are incapable of proliferating in prolonged in vitro cultures and the available Sertoli cell models have several limitations since th...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2018-10-01
|
Series: | Frontiers in Genetics |
Subjects: | |
Online Access: | https://www.frontiersin.org/article/10.3389/fgene.2018.00498/full |
id |
doaj-1f1e18b66cce44a09cc89d93d98f8238 |
---|---|
record_format |
Article |
spelling |
doaj-1f1e18b66cce44a09cc89d93d98f82382020-11-24T23:15:51ZengFrontiers Media S.A.Frontiers in Genetics1664-80212018-10-01910.3389/fgene.2018.00498418348A Human Gonadal Cell Model From Induced Pluripotent Stem CellsDaniel Rodríguez Gutiérrez0Wassim Eid1Wassim Eid2Anna Biason-Lauber3Section of Medicine, Endocrinology Division, University of Fribourg, Fribourg, SwitzerlandSection of Medicine, Endocrinology Division, University of Fribourg, Fribourg, SwitzerlandDepartment of Biochemistry, Medical Research Institute, University of Alexandria, Alexandria, EgyptSection of Medicine, Endocrinology Division, University of Fribourg, Fribourg, SwitzerlandSertoli cells are main players in the male gonads development and their study may shed light on 46,XY disorders of sex development (DSD). Mature primary Sertoli cells are incapable of proliferating in prolonged in vitro cultures and the available Sertoli cell models have several limitations since they derive from mouse or human cancer tissues. We differentiated human fibroblasts (HFs)-derived induced pluripotent stem cells into Sertoli-like cells (SLC) and, in order to characterize this new Sertoli cell model, we performed gene expression analyses by NextGeneration Sequencing techniques. This approach revealed that our putative SLC have reduced expression of pluripotency markers and expressed Sertoli cell markers such as SRY-Related HMG-Box 9 (SOX9), vimentin (VIM), and claudin-11 (CLDN-11). More in detail, the transcriptional profile analysis suggested that these cells are in an early stage of Sertoli cells maturation. Harnessing the power of induced pluripotent stem cells, we were able to generate SLC that show genetic and functional similarities to human Sertoli cells (HSerCs). SLC could become an excellent source of patient-specific Sertoli cells that could be of paramount benefit for both basic research and personalized medicine in sex development and reproductive medicine.https://www.frontiersin.org/article/10.3389/fgene.2018.00498/fullSertoli cellsiPSCNGSreprogramingcell modeldisorders/differences of sex development |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Daniel Rodríguez Gutiérrez Wassim Eid Wassim Eid Anna Biason-Lauber |
spellingShingle |
Daniel Rodríguez Gutiérrez Wassim Eid Wassim Eid Anna Biason-Lauber A Human Gonadal Cell Model From Induced Pluripotent Stem Cells Frontiers in Genetics Sertoli cells iPSC NGS reprograming cell model disorders/differences of sex development |
author_facet |
Daniel Rodríguez Gutiérrez Wassim Eid Wassim Eid Anna Biason-Lauber |
author_sort |
Daniel Rodríguez Gutiérrez |
title |
A Human Gonadal Cell Model From Induced Pluripotent Stem Cells |
title_short |
A Human Gonadal Cell Model From Induced Pluripotent Stem Cells |
title_full |
A Human Gonadal Cell Model From Induced Pluripotent Stem Cells |
title_fullStr |
A Human Gonadal Cell Model From Induced Pluripotent Stem Cells |
title_full_unstemmed |
A Human Gonadal Cell Model From Induced Pluripotent Stem Cells |
title_sort |
human gonadal cell model from induced pluripotent stem cells |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Genetics |
issn |
1664-8021 |
publishDate |
2018-10-01 |
description |
Sertoli cells are main players in the male gonads development and their study may shed light on 46,XY disorders of sex development (DSD). Mature primary Sertoli cells are incapable of proliferating in prolonged in vitro cultures and the available Sertoli cell models have several limitations since they derive from mouse or human cancer tissues. We differentiated human fibroblasts (HFs)-derived induced pluripotent stem cells into Sertoli-like cells (SLC) and, in order to characterize this new Sertoli cell model, we performed gene expression analyses by NextGeneration Sequencing techniques. This approach revealed that our putative SLC have reduced expression of pluripotency markers and expressed Sertoli cell markers such as SRY-Related HMG-Box 9 (SOX9), vimentin (VIM), and claudin-11 (CLDN-11). More in detail, the transcriptional profile analysis suggested that these cells are in an early stage of Sertoli cells maturation. Harnessing the power of induced pluripotent stem cells, we were able to generate SLC that show genetic and functional similarities to human Sertoli cells (HSerCs). SLC could become an excellent source of patient-specific Sertoli cells that could be of paramount benefit for both basic research and personalized medicine in sex development and reproductive medicine. |
topic |
Sertoli cells iPSC NGS reprograming cell model disorders/differences of sex development |
url |
https://www.frontiersin.org/article/10.3389/fgene.2018.00498/full |
work_keys_str_mv |
AT danielrodriguezgutierrez ahumangonadalcellmodelfrominducedpluripotentstemcells AT wassimeid ahumangonadalcellmodelfrominducedpluripotentstemcells AT wassimeid ahumangonadalcellmodelfrominducedpluripotentstemcells AT annabiasonlauber ahumangonadalcellmodelfrominducedpluripotentstemcells AT danielrodriguezgutierrez humangonadalcellmodelfrominducedpluripotentstemcells AT wassimeid humangonadalcellmodelfrominducedpluripotentstemcells AT wassimeid humangonadalcellmodelfrominducedpluripotentstemcells AT annabiasonlauber humangonadalcellmodelfrominducedpluripotentstemcells |
_version_ |
1725589158037028864 |