Effect of Nigella sativa on ischemia-reperfusion induced rat kidney damage

• Main Authors: Shahrzad Havakhah, Hamid R Sadeghnia, Mosa-Al-Reza Hajzadeh, Nama Mohammadian Roshan, Somayeh Shafiee, Hossein Hosseinzadeh, Narges Mohareri, Abolfazl Khajavi Rad
• Format: Article
• Language: English
• Published: Mashhad University of Medical Sciences 2015-12-01
• Series: Iranian Journal of Basic Medical Sciences
• Subjects: Comet assay Ischemia-reperfusion injury (IRI) Kidney Nigella sativa Oxidative stress
• Online Access: http://ijbms.mums.ac.ir/pdf_3856_be54e5fa3a2d2f51679f6b6b825ff388.html

Objective(s):There are a few previously reported studies about the effect of Nigella sativa oil on renal ischemia-reperfusion injury (IRI). The aim of the present study was to test the hypothesis whether pre- or post-treatment with N. sativa hydroalcoholic extract (NSE) would reduce tissue injury and oxidative damages in a clinically relevant rat model of renal IRI.    Materials and Methods: IRI was induced by clamping of bilateral renal arteries for 40 min fallowed by reperfusion for 180 min. NSE was prepared in a Soxhlet extractor and administrated with doses of 150 mg/kg or 300 mg/kg at 1 hr before ischemia induction (P-150 and 300) or at the beginning of reperfusion phase (T-150 and 300), via jugular catheter intravenously. The kidneys were then removed and subjected to biochemical analysis, comet assay or histopathological examination. Results: The kidneys of untreated IRI rats had a higher histopathological score (P