Effect of Nigella sativa on ischemia-reperfusion induced rat kidney damage
Objective(s):There are a few previously reported studies about the effect of Nigella sativa oil on renal ischemia-reperfusion injury (IRI). The aim of the present study was to test the hypothesis whether pre- or post-treatment with N. sativa hydroalcoholic extract (NSE) would reduce tissue injury an...
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doaj-1f2c0bc9c6f04b7897c867b771290cb62020-11-24T23:28:11ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences 2008-38662008-38742015-12-0117129869923856Effect of Nigella sativa on ischemia-reperfusion induced rat kidney damageShahrzad Havakhah0Hamid R Sadeghnia1Mosa-Al-Reza Hajzadeh2Nama Mohammadian Roshan3Somayeh Shafiee4Hossein Hosseinzadeh5Narges Mohareri6Abolfazl Khajavi Rad7Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranPharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranNeurocognitive Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Pathology, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranPharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranNeuroinflammation Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranObjective(s):There are a few previously reported studies about the effect of Nigella sativa oil on renal ischemia-reperfusion injury (IRI). The aim of the present study was to test the hypothesis whether pre- or post-treatment with N. sativa hydroalcoholic extract (NSE) would reduce tissue injury and oxidative damages in a clinically relevant rat model of renal IRI. Materials and Methods: IRI was induced by clamping of bilateral renal arteries for 40 min fallowed by reperfusion for 180 min. NSE was prepared in a Soxhlet extractor and administrated with doses of 150 mg/kg or 300 mg/kg at 1 hr before ischemia induction (P-150 and 300) or at the beginning of reperfusion phase (T-150 and 300), via jugular catheter intravenously. The kidneys were then removed and subjected to biochemical analysis, comet assay or histopathological examination. Results: The kidneys of untreated IRI rats had a higher histopathological score (Phttp://ijbms.mums.ac.ir/pdf_3856_be54e5fa3a2d2f51679f6b6b825ff388.htmlComet assay Ischemia-reperfusion injury (IRI) Kidney Nigella sativa Oxidative stress |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shahrzad Havakhah Hamid R Sadeghnia Mosa-Al-Reza Hajzadeh Nama Mohammadian Roshan Somayeh Shafiee Hossein Hosseinzadeh Narges Mohareri Abolfazl Khajavi Rad |
spellingShingle |
Shahrzad Havakhah Hamid R Sadeghnia Mosa-Al-Reza Hajzadeh Nama Mohammadian Roshan Somayeh Shafiee Hossein Hosseinzadeh Narges Mohareri Abolfazl Khajavi Rad Effect of Nigella sativa on ischemia-reperfusion induced rat kidney damage Iranian Journal of Basic Medical Sciences Comet assay Ischemia-reperfusion injury (IRI) Kidney Nigella sativa Oxidative stress |
author_facet |
Shahrzad Havakhah Hamid R Sadeghnia Mosa-Al-Reza Hajzadeh Nama Mohammadian Roshan Somayeh Shafiee Hossein Hosseinzadeh Narges Mohareri Abolfazl Khajavi Rad |
author_sort |
Shahrzad Havakhah |
title |
Effect of Nigella sativa on ischemia-reperfusion induced rat kidney damage |
title_short |
Effect of Nigella sativa on ischemia-reperfusion induced rat kidney damage |
title_full |
Effect of Nigella sativa on ischemia-reperfusion induced rat kidney damage |
title_fullStr |
Effect of Nigella sativa on ischemia-reperfusion induced rat kidney damage |
title_full_unstemmed |
Effect of Nigella sativa on ischemia-reperfusion induced rat kidney damage |
title_sort |
effect of nigella sativa on ischemia-reperfusion induced rat kidney damage |
publisher |
Mashhad University of Medical Sciences |
series |
Iranian Journal of Basic Medical Sciences |
issn |
2008-3866 2008-3874 |
publishDate |
2015-12-01 |
description |
Objective(s):There are a few previously reported studies about the effect of Nigella sativa oil on renal ischemia-reperfusion injury (IRI). The aim of the present study was to test the hypothesis whether pre- or post-treatment with N. sativa hydroalcoholic extract (NSE) would reduce tissue injury and oxidative damages in a clinically relevant rat model of renal IRI. Materials and Methods: IRI was induced by clamping of bilateral renal arteries for 40 min fallowed by reperfusion for 180 min. NSE was prepared in a Soxhlet extractor and administrated with doses of 150 mg/kg or 300 mg/kg at 1 hr before ischemia induction (P-150 and 300) or at the beginning of reperfusion phase (T-150 and 300), via jugular catheter intravenously. The kidneys were then removed and subjected to biochemical analysis, comet assay or histopathological examination. Results: The kidneys of untreated IRI rats had a higher histopathological score (P |
topic |
Comet assay Ischemia-reperfusion injury (IRI) Kidney Nigella sativa Oxidative stress |
url |
http://ijbms.mums.ac.ir/pdf_3856_be54e5fa3a2d2f51679f6b6b825ff388.html |
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