Akkermansia muciniphila Exerts Strain-Specific Effects on DSS-Induced Ulcerative Colitis in Mice
Akkermansia muciniphila is a commensal bacterium of the gut mucus layer. Although both in vitro and in vivo data have shown that A. muciniphila strains exhibit strain-specific modulation of gut functions, its ability to moderate immunity to ulcerative colitis have not been verified. We selected thre...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2021-08-01
|
Series: | Frontiers in Cellular and Infection Microbiology |
Subjects: | |
Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2021.698914/full |
id |
doaj-1f3e3cfdef584fa09642f2950f1f462c |
---|---|
record_format |
Article |
spelling |
doaj-1f3e3cfdef584fa09642f2950f1f462c2021-08-04T07:47:42ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882021-08-011110.3389/fcimb.2021.698914698914Akkermansia muciniphila Exerts Strain-Specific Effects on DSS-Induced Ulcerative Colitis in MiceQing Liu0Qing Liu1Wenwei Lu2Wenwei Lu3Fengwei Tian4Fengwei Tian5Jianxin Zhao6Jianxin Zhao7Hao Zhang8Hao Zhang9Hao Zhang10Hao Zhang11Kan Hong12Leilei Yu13Leilei Yu14State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, ChinaSchool of Food Science and Technology, Jiangnan University, Wuxi, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, ChinaSchool of Food Science and Technology, Jiangnan University, Wuxi, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, ChinaSchool of Food Science and Technology, Jiangnan University, Wuxi, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, ChinaSchool of Food Science and Technology, Jiangnan University, Wuxi, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, ChinaSchool of Food Science and Technology, Jiangnan University, Wuxi, ChinaNational Engineering Research Center for Functional Food, Jiangnan University, Wuxi, ChinaWuxi Translational Medicine Research Center and Jiangsu Translational Medicine Research Institute Wuxi Branch, Wuxi, ChinaWuxi People’s Hospital Affiliated to Nanjing Medical University, Wuxi, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, ChinaSchool of Food Science and Technology, Jiangnan University, Wuxi, ChinaAkkermansia muciniphila is a commensal bacterium of the gut mucus layer. Although both in vitro and in vivo data have shown that A. muciniphila strains exhibit strain-specific modulation of gut functions, its ability to moderate immunity to ulcerative colitis have not been verified. We selected three isolated human A. muciniphila strains (FSDLZ39M14, FSDLZ36M5 and FSDLZ20M4) and the A. muciniphila type strain ATCC BAA-835 to examine the effects of different A. muciniphila strains on dextran sulfate sodium-induced colitis. All of the A. muciniphila strains were cultured anaerobically in brain heart infusion medium supplemented with 0.25% type II mucin from porcine stomach. To create animal models, colitis was established in C57BL/6 mice which randomly divided into six groups with 10 mice in each group by adding 3% dextran sulfate sodium to drinking water for 7 days. A. muciniphila strains were orally administered to the mice at a dose of 1 × 109 CFU. Only A. muciniphila FSDLZ36M5 exerted significant protection against ulcerative colitis (UC) by increasing the colon length, restoring body weight, decreasing gut permeability and promoting anti-inflammatory cytokine expression. However, the other strains (FSDLZ39M14, ATCC BAA-835 and FSDLZ20M4) failed to provide these effects. Notably, A. muciniphila FSDLZ20M4 showed a tendency to exacerbate inflammation according to several indicators. Gut microbiota sequencing showed that A. muciniphila FSDLZ36M5 supplementation recovered the gut microbiota of mice to a similar state to that of the control group. A comparative genomic analysis demonstrated that the positive effects of A. muciniphila FSDLZ36M5 compared with the FSDLZ20M4 strain may be associated with specific functional genes that are involved in immune defense mechanisms and protein synthesis. Our results verify the efficacy of A. muciniphila in improving UC and provide gene targets for the efficient and rapid screening of A. muciniphila strains with UC-alleviating effects.https://www.frontiersin.org/articles/10.3389/fcimb.2021.698914/fullAkkermansia muciniphilaulcerative colitisintestinal inflammationcomparative genomicsstrain-specific |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qing Liu Qing Liu Wenwei Lu Wenwei Lu Fengwei Tian Fengwei Tian Jianxin Zhao Jianxin Zhao Hao Zhang Hao Zhang Hao Zhang Hao Zhang Kan Hong Leilei Yu Leilei Yu |
spellingShingle |
Qing Liu Qing Liu Wenwei Lu Wenwei Lu Fengwei Tian Fengwei Tian Jianxin Zhao Jianxin Zhao Hao Zhang Hao Zhang Hao Zhang Hao Zhang Kan Hong Leilei Yu Leilei Yu Akkermansia muciniphila Exerts Strain-Specific Effects on DSS-Induced Ulcerative Colitis in Mice Frontiers in Cellular and Infection Microbiology Akkermansia muciniphila ulcerative colitis intestinal inflammation comparative genomics strain-specific |
author_facet |
Qing Liu Qing Liu Wenwei Lu Wenwei Lu Fengwei Tian Fengwei Tian Jianxin Zhao Jianxin Zhao Hao Zhang Hao Zhang Hao Zhang Hao Zhang Kan Hong Leilei Yu Leilei Yu |
author_sort |
Qing Liu |
title |
Akkermansia muciniphila Exerts Strain-Specific Effects on DSS-Induced Ulcerative Colitis in Mice |
title_short |
Akkermansia muciniphila Exerts Strain-Specific Effects on DSS-Induced Ulcerative Colitis in Mice |
title_full |
Akkermansia muciniphila Exerts Strain-Specific Effects on DSS-Induced Ulcerative Colitis in Mice |
title_fullStr |
Akkermansia muciniphila Exerts Strain-Specific Effects on DSS-Induced Ulcerative Colitis in Mice |
title_full_unstemmed |
Akkermansia muciniphila Exerts Strain-Specific Effects on DSS-Induced Ulcerative Colitis in Mice |
title_sort |
akkermansia muciniphila exerts strain-specific effects on dss-induced ulcerative colitis in mice |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cellular and Infection Microbiology |
issn |
2235-2988 |
publishDate |
2021-08-01 |
description |
Akkermansia muciniphila is a commensal bacterium of the gut mucus layer. Although both in vitro and in vivo data have shown that A. muciniphila strains exhibit strain-specific modulation of gut functions, its ability to moderate immunity to ulcerative colitis have not been verified. We selected three isolated human A. muciniphila strains (FSDLZ39M14, FSDLZ36M5 and FSDLZ20M4) and the A. muciniphila type strain ATCC BAA-835 to examine the effects of different A. muciniphila strains on dextran sulfate sodium-induced colitis. All of the A. muciniphila strains were cultured anaerobically in brain heart infusion medium supplemented with 0.25% type II mucin from porcine stomach. To create animal models, colitis was established in C57BL/6 mice which randomly divided into six groups with 10 mice in each group by adding 3% dextran sulfate sodium to drinking water for 7 days. A. muciniphila strains were orally administered to the mice at a dose of 1 × 109 CFU. Only A. muciniphila FSDLZ36M5 exerted significant protection against ulcerative colitis (UC) by increasing the colon length, restoring body weight, decreasing gut permeability and promoting anti-inflammatory cytokine expression. However, the other strains (FSDLZ39M14, ATCC BAA-835 and FSDLZ20M4) failed to provide these effects. Notably, A. muciniphila FSDLZ20M4 showed a tendency to exacerbate inflammation according to several indicators. Gut microbiota sequencing showed that A. muciniphila FSDLZ36M5 supplementation recovered the gut microbiota of mice to a similar state to that of the control group. A comparative genomic analysis demonstrated that the positive effects of A. muciniphila FSDLZ36M5 compared with the FSDLZ20M4 strain may be associated with specific functional genes that are involved in immune defense mechanisms and protein synthesis. Our results verify the efficacy of A. muciniphila in improving UC and provide gene targets for the efficient and rapid screening of A. muciniphila strains with UC-alleviating effects. |
topic |
Akkermansia muciniphila ulcerative colitis intestinal inflammation comparative genomics strain-specific |
url |
https://www.frontiersin.org/articles/10.3389/fcimb.2021.698914/full |
work_keys_str_mv |
AT qingliu akkermansiamuciniphilaexertsstrainspecificeffectsondssinducedulcerativecolitisinmice AT qingliu akkermansiamuciniphilaexertsstrainspecificeffectsondssinducedulcerativecolitisinmice AT wenweilu akkermansiamuciniphilaexertsstrainspecificeffectsondssinducedulcerativecolitisinmice AT wenweilu akkermansiamuciniphilaexertsstrainspecificeffectsondssinducedulcerativecolitisinmice AT fengweitian akkermansiamuciniphilaexertsstrainspecificeffectsondssinducedulcerativecolitisinmice AT fengweitian akkermansiamuciniphilaexertsstrainspecificeffectsondssinducedulcerativecolitisinmice AT jianxinzhao akkermansiamuciniphilaexertsstrainspecificeffectsondssinducedulcerativecolitisinmice AT jianxinzhao akkermansiamuciniphilaexertsstrainspecificeffectsondssinducedulcerativecolitisinmice AT haozhang akkermansiamuciniphilaexertsstrainspecificeffectsondssinducedulcerativecolitisinmice AT haozhang akkermansiamuciniphilaexertsstrainspecificeffectsondssinducedulcerativecolitisinmice AT haozhang akkermansiamuciniphilaexertsstrainspecificeffectsondssinducedulcerativecolitisinmice AT haozhang akkermansiamuciniphilaexertsstrainspecificeffectsondssinducedulcerativecolitisinmice AT kanhong akkermansiamuciniphilaexertsstrainspecificeffectsondssinducedulcerativecolitisinmice AT leileiyu akkermansiamuciniphilaexertsstrainspecificeffectsondssinducedulcerativecolitisinmice AT leileiyu akkermansiamuciniphilaexertsstrainspecificeffectsondssinducedulcerativecolitisinmice |
_version_ |
1721222394521059328 |