L-asparaginase produced from soil isolates of Pseudomonas aeruginosa shows potent anti-cancer activity on HeLa cells
Among cancers, acute lymphoblastic leukemia (ALL) occurs in the children <15 years of age. L-asparaginase is an important therapeutic enzyme used for treating ALL. Owing to its therapeutic use and demand, microorganisms have been in use for many years to produce L-asparaginase on an industrial sc...
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doaj-1f484c289515456ab9a9c4c118168e7e2020-11-24T22:01:01ZengElsevierSaudi Journal of Biological Sciences1319-562X2019-09-0126611461153L-asparaginase produced from soil isolates of Pseudomonas aeruginosa shows potent anti-cancer activity on HeLa cellsNuzhath Fatima0Mohd. Mazharuddin Khan1Imran Ali Khan2Department of Microbiology, Rayalaseema University, Kurnool, Andhra Pradesh 518007, IndiaFaculty of Natural Sciences, Department of Environmental Science, Addis Ababa University, Addis Ababa 1176, EthiopiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, PO Box-10219, Riyadh 11433, Saudi Arabia; Corresponding author.Among cancers, acute lymphoblastic leukemia (ALL) occurs in the children <15 years of age. L-asparaginase is an important therapeutic enzyme used for treating ALL. Owing to its therapeutic use and demand, microorganisms have been in use for many years to produce L-asparaginase on an industrial scale. Gram-negative bacteria (Serratia, Erwinia and Escherichia coli) species were used in L-asparaginase. However, earlier studies have documented that the long-term use of enzymes produced from these commercial strains induces hypersensitivity in patients. Therefore, there is a need to discover novel microbial strains producing L-asparaginase with anti-cancer properties, which can be employed for the commercial production of the enzyme. In this study, three strains of Pseudomonas aeruginosa (accession numbers LC425424 (P31), LC425425 (P32), and LC425426 (P34)) isolated from garden soil were screened for the invention of L-asparaginase. Fermented production media was dialyzed to attain the purified enzyme, thus showed a dose-depended cytotoxic effect on HeLa cells, as determined by MTT assay. The IC50s of the different isolates were 86.73, 57.65, and 40.34 µg/mL. These results indicate that pseudomonal L-asparaginase may be used for cancer treatment. Keywords: HeLa cells, ALL, L-asparaginase, Pseudomonas aeruginosahttp://www.sciencedirect.com/science/article/pii/S1319562X19300749 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nuzhath Fatima Mohd. Mazharuddin Khan Imran Ali Khan |
spellingShingle |
Nuzhath Fatima Mohd. Mazharuddin Khan Imran Ali Khan L-asparaginase produced from soil isolates of Pseudomonas aeruginosa shows potent anti-cancer activity on HeLa cells Saudi Journal of Biological Sciences |
author_facet |
Nuzhath Fatima Mohd. Mazharuddin Khan Imran Ali Khan |
author_sort |
Nuzhath Fatima |
title |
L-asparaginase produced from soil isolates of Pseudomonas aeruginosa shows potent anti-cancer activity on HeLa cells |
title_short |
L-asparaginase produced from soil isolates of Pseudomonas aeruginosa shows potent anti-cancer activity on HeLa cells |
title_full |
L-asparaginase produced from soil isolates of Pseudomonas aeruginosa shows potent anti-cancer activity on HeLa cells |
title_fullStr |
L-asparaginase produced from soil isolates of Pseudomonas aeruginosa shows potent anti-cancer activity on HeLa cells |
title_full_unstemmed |
L-asparaginase produced from soil isolates of Pseudomonas aeruginosa shows potent anti-cancer activity on HeLa cells |
title_sort |
l-asparaginase produced from soil isolates of pseudomonas aeruginosa shows potent anti-cancer activity on hela cells |
publisher |
Elsevier |
series |
Saudi Journal of Biological Sciences |
issn |
1319-562X |
publishDate |
2019-09-01 |
description |
Among cancers, acute lymphoblastic leukemia (ALL) occurs in the children <15 years of age. L-asparaginase is an important therapeutic enzyme used for treating ALL. Owing to its therapeutic use and demand, microorganisms have been in use for many years to produce L-asparaginase on an industrial scale. Gram-negative bacteria (Serratia, Erwinia and Escherichia coli) species were used in L-asparaginase. However, earlier studies have documented that the long-term use of enzymes produced from these commercial strains induces hypersensitivity in patients. Therefore, there is a need to discover novel microbial strains producing L-asparaginase with anti-cancer properties, which can be employed for the commercial production of the enzyme. In this study, three strains of Pseudomonas aeruginosa (accession numbers LC425424 (P31), LC425425 (P32), and LC425426 (P34)) isolated from garden soil were screened for the invention of L-asparaginase. Fermented production media was dialyzed to attain the purified enzyme, thus showed a dose-depended cytotoxic effect on HeLa cells, as determined by MTT assay. The IC50s of the different isolates were 86.73, 57.65, and 40.34 µg/mL. These results indicate that pseudomonal L-asparaginase may be used for cancer treatment. Keywords: HeLa cells, ALL, L-asparaginase, Pseudomonas aeruginosa |
url |
http://www.sciencedirect.com/science/article/pii/S1319562X19300749 |
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