Prevalence of Methicillin-Resistant Staphylococcus aureus Carrying Panton-Valentine Leukocidin Gene in Cutaneous Infections in the City of Isfahan
Background: Methicillin-Resistant Staphylococcus aureus (MRSA) is a major cause of Nosocomial and community infections that are becoming increasingly difficult to combat, because of emerging resistance to all classes of antibiotics. Moreover Panton-Valentine leukocidin (PVL) is an import...
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Tehran University of Medical Sciences
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doaj-1f4c5c7a0cb44a4da8ca0180ee6058942020-11-25T03:53:45ZengTehran University of Medical SciencesJournal of Medical Bacteriology2251-86492322-25812015-10-0111-2Prevalence of Methicillin-Resistant Staphylococcus aureus Carrying Panton-Valentine Leukocidin Gene in Cutaneous Infections in the City of IsfahanSolmaz Ohadian-Moghadam0SeyedAsghar Havaei1MohammadReza Pourmand2Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran.Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IR Iran.Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran. Background: Methicillin-Resistant Staphylococcus aureus (MRSA) is a major cause of Nosocomial and community infections that are becoming increasingly difficult to combat, because of emerging resistance to all classes of antibiotics. Moreover Panton-Valentine leukocidin (PVL) is an important virulence factor in S. aureus and causes white blood cell destruction, necrosis and accelerated apoptosis. The aim of this study was to determine the frequency of PVL-positive MRSA in cutaneous infections, for epidemiological purposes and also to determine antibiotic resistance of the isolates. Methods: Collectively, 56 isolates of S. aureus were obtained from Isfahan University of Medical sciences affiliated hospitals and confirmed with biochemical tests (coagulase, mannitol fermentation, and DNase). Then polymerase chain reaction (PCR) was used to detect pvl gene. Coagulase gene was used as internal control. The antibiotic susceptibility of all isolates to methicillin was determined using disk diffusion method. Results: Out of 56 isolates 14.3% were PVL positive that 37.5% were from abscess and 62.5% were from wound. Among all of these isolates 67.8% were MRSA and also 75% of PVL-positive isolates were MRSA. Conclusion: The prevalence of PVL positive MRSA in cutaneous isolates is high. Future works are necessary for a more complete understanding of distribution of these virulent isolates in nasal carriers to decrease the risk of infections https://jmb.tums.ac.ir/index.php/jmb/article/view/5Methicillin-ResistantStaphylococcus aureusPanton-Valentine leukocidinPolymerase Chain Reaction |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Solmaz Ohadian-Moghadam SeyedAsghar Havaei MohammadReza Pourmand |
spellingShingle |
Solmaz Ohadian-Moghadam SeyedAsghar Havaei MohammadReza Pourmand Prevalence of Methicillin-Resistant Staphylococcus aureus Carrying Panton-Valentine Leukocidin Gene in Cutaneous Infections in the City of Isfahan Journal of Medical Bacteriology Methicillin-Resistant Staphylococcus aureus Panton-Valentine leukocidin Polymerase Chain Reaction |
author_facet |
Solmaz Ohadian-Moghadam SeyedAsghar Havaei MohammadReza Pourmand |
author_sort |
Solmaz Ohadian-Moghadam |
title |
Prevalence of Methicillin-Resistant Staphylococcus aureus Carrying Panton-Valentine Leukocidin Gene in Cutaneous Infections in the City of Isfahan |
title_short |
Prevalence of Methicillin-Resistant Staphylococcus aureus Carrying Panton-Valentine Leukocidin Gene in Cutaneous Infections in the City of Isfahan |
title_full |
Prevalence of Methicillin-Resistant Staphylococcus aureus Carrying Panton-Valentine Leukocidin Gene in Cutaneous Infections in the City of Isfahan |
title_fullStr |
Prevalence of Methicillin-Resistant Staphylococcus aureus Carrying Panton-Valentine Leukocidin Gene in Cutaneous Infections in the City of Isfahan |
title_full_unstemmed |
Prevalence of Methicillin-Resistant Staphylococcus aureus Carrying Panton-Valentine Leukocidin Gene in Cutaneous Infections in the City of Isfahan |
title_sort |
prevalence of methicillin-resistant staphylococcus aureus carrying panton-valentine leukocidin gene in cutaneous infections in the city of isfahan |
publisher |
Tehran University of Medical Sciences |
series |
Journal of Medical Bacteriology |
issn |
2251-8649 2322-2581 |
publishDate |
2015-10-01 |
description |
Background: Methicillin-Resistant Staphylococcus aureus (MRSA) is a major cause of Nosocomial and community infections that are becoming increasingly difficult to combat, because of emerging resistance to all classes of antibiotics. Moreover Panton-Valentine leukocidin (PVL) is an important virulence factor in S. aureus and causes white blood cell destruction, necrosis and accelerated apoptosis. The aim of this study was to determine the frequency of PVL-positive MRSA in cutaneous infections, for epidemiological purposes and also to determine antibiotic resistance of the isolates.
Methods: Collectively, 56 isolates of S. aureus were obtained from Isfahan University of Medical sciences affiliated hospitals and confirmed with biochemical tests (coagulase, mannitol fermentation, and DNase). Then polymerase chain reaction (PCR) was used to detect pvl gene. Coagulase gene was used as internal control. The antibiotic susceptibility of all isolates to methicillin was determined using disk diffusion method.
Results: Out of 56 isolates 14.3% were PVL positive that 37.5% were from abscess and 62.5% were from wound. Among all of these isolates 67.8% were MRSA and also 75% of PVL-positive isolates were MRSA.
Conclusion: The prevalence of PVL positive MRSA in cutaneous isolates is high. Future works are necessary for a more complete understanding of distribution of these virulent isolates in nasal carriers to decrease the risk of infections
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topic |
Methicillin-Resistant Staphylococcus aureus Panton-Valentine leukocidin Polymerase Chain Reaction |
url |
https://jmb.tums.ac.ir/index.php/jmb/article/view/5 |
work_keys_str_mv |
AT solmazohadianmoghadam prevalenceofmethicillinresistantstaphylococcusaureuscarryingpantonvalentineleukocidingeneincutaneousinfectionsinthecityofisfahan AT seyedasgharhavaei prevalenceofmethicillinresistantstaphylococcusaureuscarryingpantonvalentineleukocidingeneincutaneousinfectionsinthecityofisfahan AT mohammadrezapourmand prevalenceofmethicillinresistantstaphylococcusaureuscarryingpantonvalentineleukocidingeneincutaneousinfectionsinthecityofisfahan |
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