The mutational impact of culturing human pluripotent and adult stem cells

The mutational impact of culturing human pluripotent and adult stem cells

Genetic changes acquired during in vitro culture pose a challenge to application of stem cells. Here the authors use whole genome sequencing to show that cultured human adult and pluripotent stem cells have a high mutational load caused by oxidative stress and reduced oxygen tension in culture lower...

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Main Authors: Ewart Kuijk, Myrthe Jager, Bastiaan van der Roest, Mauro D. Locati, Arne Van Hoeck, Jerome Korzelius, Roel Janssen, Nicolle Besselink, Sander Boymans, Ruben van Boxtel, Edwin Cuppen
Format: Article
Language:English
Published: Nature Publishing Group 2020-05-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-16323-4
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spelling doaj-1f6a475491464666a79c0e3f7512c41e2021-05-23T11:15:26ZengNature Publishing GroupNature Communications2041-17232020-05-0111111210.1038/s41467-020-16323-4The mutational impact of culturing human pluripotent and adult stem cellsEwart Kuijk0Myrthe Jager1Bastiaan van der Roest2Mauro D. Locati3Arne Van Hoeck4Jerome Korzelius5Roel Janssen6Nicolle Besselink7Sander Boymans8Ruben van Boxtel9Edwin Cuppen10Center for Molecular Medicine and Oncode Institute, University Medical Center Utrecht, Universiteitsweg 100Center for Molecular Medicine and Oncode Institute, University Medical Center Utrecht, Universiteitsweg 100Center for Molecular Medicine and Oncode Institute, University Medical Center Utrecht, Universiteitsweg 100Center for Molecular Medicine and Oncode Institute, University Medical Center Utrecht, Universiteitsweg 100Center for Molecular Medicine and Oncode Institute, University Medical Center Utrecht, Universiteitsweg 100Leibniz Institute on Aging—Fritz Lipmann Institute, Beutenbergstraße 11Center for Molecular Medicine and Oncode Institute, University Medical Center Utrecht, Universiteitsweg 100Center for Molecular Medicine and Oncode Institute, University Medical Center Utrecht, Universiteitsweg 100Center for Molecular Medicine and Oncode Institute, University Medical Center Utrecht, Universiteitsweg 100Princess Máxima Center for Pediatric Oncology, Heidelberglaan 25, 3584 CS UtrechtCenter for Molecular Medicine and Oncode Institute, University Medical Center Utrecht, Universiteitsweg 100Genetic changes acquired during in vitro culture pose a challenge to application of stem cells. Here the authors use whole genome sequencing to show that cultured human adult and pluripotent stem cells have a high mutational load caused by oxidative stress and reduced oxygen tension in culture lowers mutation rates.https://doi.org/10.1038/s41467-020-16323-4
collection DOAJ
language English
format Article
sources DOAJ
author Ewart Kuijk
Myrthe Jager
Bastiaan van der Roest
Mauro D. Locati
Arne Van Hoeck
Jerome Korzelius
Roel Janssen
Nicolle Besselink
Sander Boymans
Ruben van Boxtel
Edwin Cuppen
spellingShingle Ewart Kuijk
Myrthe Jager
Bastiaan van der Roest
Mauro D. Locati
Arne Van Hoeck
Jerome Korzelius
Roel Janssen
Nicolle Besselink
Sander Boymans
Ruben van Boxtel
Edwin Cuppen
The mutational impact of culturing human pluripotent and adult stem cells
Nature Communications
author_facet Ewart Kuijk
Myrthe Jager
Bastiaan van der Roest
Mauro D. Locati
Arne Van Hoeck
Jerome Korzelius
Roel Janssen
Nicolle Besselink
Sander Boymans
Ruben van Boxtel
Edwin Cuppen
author_sort Ewart Kuijk
title The mutational impact of culturing human pluripotent and adult stem cells
title_short The mutational impact of culturing human pluripotent and adult stem cells
title_full The mutational impact of culturing human pluripotent and adult stem cells
title_fullStr The mutational impact of culturing human pluripotent and adult stem cells
title_full_unstemmed The mutational impact of culturing human pluripotent and adult stem cells
title_sort mutational impact of culturing human pluripotent and adult stem cells
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2020-05-01
description Genetic changes acquired during in vitro culture pose a challenge to application of stem cells. Here the authors use whole genome sequencing to show that cultured human adult and pluripotent stem cells have a high mutational load caused by oxidative stress and reduced oxygen tension in culture lowers mutation rates.
url https://doi.org/10.1038/s41467-020-16323-4
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