Solving the mysteries of the bacterial cell – application of novel techniques in fluorescence microscopy

We have reviewed how the development of fluorescent markers, triggered by the discovery of green fluorescence protein and its other color variants leading to the establishment of methods for studies of protein interactions with application of fluorescent proteins, affected the view of bacterial cell...

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Main Authors:	Magdalena Donczew, Katarzyna Ginda, Jolanta Zakrzewska-Czerwińska, Dagmara Jakimowicz
Format:	Article
Language:	English
Published:	Index Copernicus International S.A. 2011-01-01
Series:	Postępy Higieny i Medycyny Doświadczalnej
Subjects:	fluorescence microscopy fluorescent proteins FRET FRAP bacterial cytoskeleton organization of bacterial cell
Online Access:	http://journals.indexcopernicus.com/fulltxt.php?ICID=933713

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spelling	doaj-1f6c97defc78484695142798593fab2f2020-11-24T23:32:26ZengIndex Copernicus International S.A.Postępy Higieny i Medycyny Doświadczalnej0032-54491732-26932011-01-0165114123Solving the mysteries of the bacterial cell – application of novel techniques in fluorescence microscopyMagdalena DonczewKatarzyna GindaJolanta Zakrzewska-CzerwińskaDagmara JakimowiczWe have reviewed how the development of fluorescent markers, triggered by the discovery of green fluorescence protein and its other color variants leading to the establishment of methods for studies of protein interactions with application of fluorescent proteins, affected the view of bacterial cell organization. Application of the new microscopic methods allowed localization of proteins and chromosomal regions, and observation of their migration in real time. These studies revealed the spatial organization of bacterial cells which includes specific subcellular localization of proteins, the presence of dynamic cytoskeletal structures, orchestrated and active segregation of chromosomes, and spatiotemporal gene regulation.http://journals.indexcopernicus.com/fulltxt.php?ICID=933713fluorescence microscopyfluorescent proteinsFRETFRAPbacterial cytoskeletonorganization of bacterial cell
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Magdalena Donczew Katarzyna Ginda Jolanta Zakrzewska-Czerwińska Dagmara Jakimowicz
spellingShingle	Magdalena Donczew Katarzyna Ginda Jolanta Zakrzewska-Czerwińska Dagmara Jakimowicz Solving the mysteries of the bacterial cell – application of novel techniques in fluorescence microscopy Postępy Higieny i Medycyny Doświadczalnej fluorescence microscopy fluorescent proteins FRET FRAP bacterial cytoskeleton organization of bacterial cell
author_facet	Magdalena Donczew Katarzyna Ginda Jolanta Zakrzewska-Czerwińska Dagmara Jakimowicz
author_sort	Magdalena Donczew
title	Solving the mysteries of the bacterial cell – application of novel techniques in fluorescence microscopy
title_short	Solving the mysteries of the bacterial cell – application of novel techniques in fluorescence microscopy
title_full	Solving the mysteries of the bacterial cell – application of novel techniques in fluorescence microscopy
title_fullStr	Solving the mysteries of the bacterial cell – application of novel techniques in fluorescence microscopy
title_full_unstemmed	Solving the mysteries of the bacterial cell – application of novel techniques in fluorescence microscopy
title_sort	solving the mysteries of the bacterial cell – application of novel techniques in fluorescence microscopy
publisher	Index Copernicus International S.A.
series	Postępy Higieny i Medycyny Doświadczalnej
issn	0032-5449 1732-2693
publishDate	2011-01-01
description	We have reviewed how the development of fluorescent markers, triggered by the discovery of green fluorescence protein and its other color variants leading to the establishment of methods for studies of protein interactions with application of fluorescent proteins, affected the view of bacterial cell organization. Application of the new microscopic methods allowed localization of proteins and chromosomal regions, and observation of their migration in real time. These studies revealed the spatial organization of bacterial cells which includes specific subcellular localization of proteins, the presence of dynamic cytoskeletal structures, orchestrated and active segregation of chromosomes, and spatiotemporal gene regulation.
topic	fluorescence microscopy fluorescent proteins FRET FRAP bacterial cytoskeleton organization of bacterial cell
url	http://journals.indexcopernicus.com/fulltxt.php?ICID=933713
work_keys_str_mv	AT magdalenadonczew solvingthemysteriesofthebacterialcellapplicationofnoveltechniquesinfluorescencemicroscopy AT katarzynaginda solvingthemysteriesofthebacterialcellapplicationofnoveltechniquesinfluorescencemicroscopy AT jolantazakrzewskaczerwinska solvingthemysteriesofthebacterialcellapplicationofnoveltechniquesinfluorescencemicroscopy AT dagmarajakimowicz solvingthemysteriesofthebacterialcellapplicationofnoveltechniquesinfluorescencemicroscopy
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Solving the mysteries of the bacterial cell – application of novel techniques in fluorescence microscopy

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