Dudomycins: New Secondary Metabolites Produced After Heterologous Expression of an Nrps Cluster from <i>Streptomyces albus</i> ssp. <i>Chlorinus</i> Nrrl B-24108

• Main Authors: Constanze Lasch, Marc Stierhof, Marta Rodríguez Estévez, Maksym Myronovskyi, Josef Zapp, Andriy Luzhetskyy
• Format: Article
• Language: English
• Published: MDPI AG 2020-11-01
• Series: Microorganisms
• Subjects: Streptomyces; NRPS; heterologous expression
• Online Access: https://www.mdpi.com/2076-2607/8/11/1800

Since the 1950s, natural products of bacterial origin were systematically developed to be used as drugs with a wide range of medical applications. The available treatment options for many diseases are still not satisfying, wherefore, the discovery of new structures has not lost any of its importance. Beyond the great variety of already isolated and characterized metabolites, Streptomycetes still harbor uninvestigated gene clusters whose products can be accessed using heterologous expression in host organisms. This works presents the discovery of a set of structurally novel secondary metabolites, dudomycins A to D, through the expression of a cryptic NRPS cluster from <i>Streptomyces albus</i> ssp. <i>Chlorinus </i>NRRL B-24108 in the heterologous host strain <i>Streptomyces albus</i> Del14. A minimal set of genes, required for the production of dudomycins, was defined through gene inactivation experiments. This paper also proposes a model for dudomycin biosynthesis.