Effects of Chrysin plant flavonoid on proliferation and apoptosis of gastric cancer cell line (AGS)
Introduction: Chrysin is a natural flavonoid antioxidant which its role in tumor cell death has been reported. The aim of this study was to investigate the mechanism of Chrysin effect on proliferation and apoptosis of gastric cancer cell line (AGS). Methods: Cells were cultured and treated with diff...
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doaj-1f6df575fddc47638361983da3d93c662021-08-02T01:07:37ZengNikan Research InstituteJournal of HerbMed Pharmacology2345-50042014-04-0132125128JHP_20150527170155Effects of Chrysin plant flavonoid on proliferation and apoptosis of gastric cancer cell line (AGS)Askarian Dehkordi Narges0Shirzad Hedayatollah1Salimzadeh Loghman2Zamanzad Behnam3Meshkate Rezvan4Kiyani Mahbube5Department of Immunology, Shahrekord University of Medical Sciences, Shahrekord, IranCell and Molecular, Research Center, Shahrekord University of Medical Sciences, Shahrekord, IranMedical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, IranCell and Molecular, Research Center, Shahrekord University of Medical Sciences, Shahrekord, IranDepartment of Immunology, Shahrekord University of Medical Sciences, Shahrekord, IranDepartment of Immunology, Shahrekord University of Medical Sciences, Shahrekord, IranIntroduction: Chrysin is a natural flavonoid antioxidant which its role in tumor cell death has been reported. The aim of this study was to investigate the mechanism of Chrysin effect on proliferation and apoptosis of gastric cancer cell line (AGS). Methods: Cells were cultured and treated with different concentrations of Chrysin (90, 80, 70, 60, 50 μM) for 48 h and evaluated for cell viability. To examine the cytotoxic effect of drug in inducing apoptosis, staining with fluorescein isothiocyanate (FITC) and propidium iodide (PI) was used. The cells were treated for 48 h with different concentrations of Chrysin (50 of 90 μM) and examined for the morphological changes. The one-way analysis of variance (ANOVA) and the Excel software were used for data analysis. Results: Different concentrations of Chrysin significantly inhibited the growth and proliferation of AGS cells (p<0.05). The IC50 dose was determined to be 67.5±0.66. Apoptosis induced by 50 and 70 μM of Chrysin was significantly greater than in untreated cells (p<0.05). Cells treated with high concentration of Chrysin (90 μM) showed more prominent growth inhibition and cell shrinkage compared to cells treated with the lower concentrations of Chrysin. Conclusion: The results of this study showed that Chrysin effects on AGS cell line were significantly high and dose-dependent and might be helpful in the treatment of gastric cancer. Chrysin may therefore be considered a potential candidate for both cancer prevention and treatment. Further investigation is needed to validate the contribution of chrysin in tumor therapy in vivo.http://www.herbmedpharmacol.com/PDF/JHP-3-125.pdfChrysinGastric cancerApoptosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Askarian Dehkordi Narges Shirzad Hedayatollah Salimzadeh Loghman Zamanzad Behnam Meshkate Rezvan Kiyani Mahbube |
spellingShingle |
Askarian Dehkordi Narges Shirzad Hedayatollah Salimzadeh Loghman Zamanzad Behnam Meshkate Rezvan Kiyani Mahbube Effects of Chrysin plant flavonoid on proliferation and apoptosis of gastric cancer cell line (AGS) Journal of HerbMed Pharmacology Chrysin Gastric cancer Apoptosis |
author_facet |
Askarian Dehkordi Narges Shirzad Hedayatollah Salimzadeh Loghman Zamanzad Behnam Meshkate Rezvan Kiyani Mahbube |
author_sort |
Askarian Dehkordi Narges |
title |
Effects of Chrysin plant flavonoid on proliferation and apoptosis of gastric cancer cell line (AGS) |
title_short |
Effects of Chrysin plant flavonoid on proliferation and apoptosis of gastric cancer cell line (AGS) |
title_full |
Effects of Chrysin plant flavonoid on proliferation and apoptosis of gastric cancer cell line (AGS) |
title_fullStr |
Effects of Chrysin plant flavonoid on proliferation and apoptosis of gastric cancer cell line (AGS) |
title_full_unstemmed |
Effects of Chrysin plant flavonoid on proliferation and apoptosis of gastric cancer cell line (AGS) |
title_sort |
effects of chrysin plant flavonoid on proliferation and apoptosis of gastric cancer cell line (ags) |
publisher |
Nikan Research Institute |
series |
Journal of HerbMed Pharmacology |
issn |
2345-5004 |
publishDate |
2014-04-01 |
description |
Introduction: Chrysin is a natural flavonoid antioxidant which its role in tumor cell death has been reported. The aim of this study was to investigate the mechanism of Chrysin effect on proliferation and apoptosis of gastric cancer cell line (AGS). Methods: Cells were cultured and treated with different concentrations of Chrysin (90, 80, 70, 60, 50 μM) for 48 h and evaluated for cell viability. To examine the cytotoxic effect of drug in inducing apoptosis, staining with fluorescein isothiocyanate (FITC) and propidium iodide (PI) was used. The cells were treated for 48 h with different concentrations of Chrysin (50 of 90 μM) and examined for the morphological changes. The one-way analysis of variance (ANOVA) and the Excel software were used for data analysis. Results: Different concentrations of Chrysin significantly inhibited the growth and proliferation of AGS cells (p<0.05). The IC50 dose was determined to be 67.5±0.66. Apoptosis induced by 50 and 70 μM of Chrysin was significantly greater than in untreated cells (p<0.05). Cells treated with high concentration of Chrysin (90 μM) showed more prominent growth inhibition and cell shrinkage compared to cells treated with the lower concentrations of Chrysin. Conclusion: The results of this study showed that Chrysin effects on AGS cell line were significantly high and dose-dependent and might be helpful in the treatment of gastric cancer. Chrysin may therefore be considered a potential candidate for both cancer prevention and treatment. Further investigation is needed to validate the contribution of chrysin in tumor therapy in vivo. |
topic |
Chrysin Gastric cancer Apoptosis |
url |
http://www.herbmedpharmacol.com/PDF/JHP-3-125.pdf |
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