Polymorphisms of POU1F1 and STAT5A genes and their associate on with milk production traits in cattle
Specific trait candidate genes are sequenced genes with known biological activity. The effects of POU1F1 and STAT5A on milk production traits have been studied in several studies. POU1F1 affects on transcription of prolactin and growth hormone gene, as well as, STAT5A is known as a main mediator of...
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Ferdowsi University of Mashhad
2015-04-01
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Online Access: | http://ijasr.um.ac.ir/index.php/animal/article/view/28771 |
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doaj-1f8e242f8f294d8d876ec8a691c16e562021-05-02T19:31:57ZfasFerdowsi University of Mashhadپژوهشهای علوم دامی ایران2008-31062423-40012015-04-01643883948708Polymorphisms of POU1F1 and STAT5A genes and their associate on with milk production traits in cattleSonia Zakizadeh0Ramin Haeri1Akbar Soleimani2Dept. of Animal Science, Higher Applied Science-Technology InstituteM.Sc. Graduated, Dept. of Animal Science, Islamic Azad University, Kashmar BranchIslamic Azad University, Kashmar BranchSpecific trait candidate genes are sequenced genes with known biological activity. The effects of POU1F1 and STAT5A on milk production traits have been studied in several studies. POU1F1 affects on transcription of prolactin and growth hormone gene, as well as, STAT5A is known as a main mediator of growth hormone action on target genes and intracellular mediator of prolactin signaling. Since these genes are essential for development of mammary system, the aim of this study was to determine association of their polymorphism with milk production breeding values in Brown Swiss cattle. Blood of ninety milking cow were randomly obtained. DNA was extracted from whole blood using modified salting out method, then the desired fragments were PCR amplified and digested by specific restriction endonuclease enzymes. Gene and genotype frequencies, heterozygosity indexes, the real and effective allele number were calculated by PopGene software; and the breeding values of production traits were estimated by DFREML. SAS software was used to analyze association between genotypes and breeding values. The frequency of 'A' and 'C' alleles of POU1F1 and STAT5A were 0.455 and 0.489, respectively. This population was in hardy-weinburg equilibrium for both loci. There was no significant association between genotypes and breeding values, although POU1F1*B tended to produce higher milk and POU1F1*A showed higher fat and protein percent.http://ijasr.um.ac.ir/index.php/animal/article/view/28771POU1F1STAT5Abreeding valueBrown Swissmilk production traits |
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DOAJ |
language |
fas |
format |
Article |
sources |
DOAJ |
author |
Sonia Zakizadeh Ramin Haeri Akbar Soleimani |
spellingShingle |
Sonia Zakizadeh Ramin Haeri Akbar Soleimani Polymorphisms of POU1F1 and STAT5A genes and their associate on with milk production traits in cattle پژوهشهای علوم دامی ایران POU1F1 STAT5A breeding value Brown Swiss milk production traits |
author_facet |
Sonia Zakizadeh Ramin Haeri Akbar Soleimani |
author_sort |
Sonia Zakizadeh |
title |
Polymorphisms of POU1F1 and STAT5A genes and their associate on with milk production traits in cattle |
title_short |
Polymorphisms of POU1F1 and STAT5A genes and their associate on with milk production traits in cattle |
title_full |
Polymorphisms of POU1F1 and STAT5A genes and their associate on with milk production traits in cattle |
title_fullStr |
Polymorphisms of POU1F1 and STAT5A genes and their associate on with milk production traits in cattle |
title_full_unstemmed |
Polymorphisms of POU1F1 and STAT5A genes and their associate on with milk production traits in cattle |
title_sort |
polymorphisms of pou1f1 and stat5a genes and their associate on with milk production traits in cattle |
publisher |
Ferdowsi University of Mashhad |
series |
پژوهشهای علوم دامی ایران |
issn |
2008-3106 2423-4001 |
publishDate |
2015-04-01 |
description |
Specific trait candidate genes are sequenced genes with known biological activity. The effects of POU1F1 and STAT5A on milk production traits have been studied in several studies. POU1F1 affects on transcription of prolactin and growth hormone gene, as well as, STAT5A is known as a main mediator of growth hormone action on target genes and intracellular mediator of prolactin signaling. Since these genes are essential for development of mammary system, the aim of this study was to determine association of their polymorphism with milk production breeding values in Brown Swiss cattle. Blood of ninety milking cow were randomly obtained. DNA was extracted from whole blood using modified salting out method, then the desired fragments were PCR amplified and digested by specific restriction endonuclease enzymes. Gene and genotype frequencies, heterozygosity indexes, the real and effective allele number were calculated by PopGene software; and the breeding values of production traits were estimated by DFREML. SAS software was used to analyze association between genotypes and breeding values. The frequency of 'A' and 'C' alleles of POU1F1 and STAT5A were 0.455 and 0.489, respectively. This population was in hardy-weinburg equilibrium for both loci. There was no significant association between genotypes and breeding values, although POU1F1*B tended to produce higher milk and POU1F1*A showed higher fat and protein percent. |
topic |
POU1F1 STAT5A breeding value Brown Swiss milk production traits |
url |
http://ijasr.um.ac.ir/index.php/animal/article/view/28771 |
work_keys_str_mv |
AT soniazakizadeh polymorphismsofpou1f1andstat5agenesandtheirassociateonwithmilkproductiontraitsincattle AT raminhaeri polymorphismsofpou1f1andstat5agenesandtheirassociateonwithmilkproductiontraitsincattle AT akbarsoleimani polymorphismsofpou1f1andstat5agenesandtheirassociateonwithmilkproductiontraitsincattle |
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