Design of Primers for Detection and Typing of β-Lactamase Genes from Pathogenic Species of <i>Burkholderia</i>

The set of oligonucleotide primers was designed to identify β-lactam-resitance determinats in isolates of pathogenic Burkholderia using PCR. Simultaneously identified was certain molecular class of detected β-lactamases. PCR with primers specific to metallo-β-lactamase (class B) and oxacillinase of...

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Main Authors: A. V. Romanova, I. B. Zakharova, V. S. Zamaraev, D. V. Viktorov
Format: Article
Language:Russian
Published: Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” 2012-04-01
Series:Проблемы особо опасных инфекций
Subjects:
Online Access:https://journal.microbe.ru/jour/article/view/797
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spelling doaj-1f8e4ae8553d4183ad875ff37d117a952021-07-22T07:51:34ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2012-04-0102(112)596110.21055/0370-1069-2012-2(112)-59-61781Design of Primers for Detection and Typing of β-Lactamase Genes from Pathogenic Species of <i>Burkholderia</i>A. V. Romanova0I. B. Zakharova1V. S. Zamaraev2D. V. Viktorov3Volgograd Research Anti-Plague InstituteVolgograd Research Anti-Plague InstituteVolgograd Research Anti-Plague InstituteVolgograd Research Anti-Plague InstituteThe set of oligonucleotide primers was designed to identify β-lactam-resitance determinats in isolates of pathogenic Burkholderia using PCR. Simultaneously identified was certain molecular class of detected β-lactamases. PCR with primers specific to metallo-β-lactamase (class B) and oxacillinase of D-ala carboxypeptidase family (class D of β-lactamase) gene sequences allowed to differentiate among pseudomallei group of Burkholderia species.https://journal.microbe.ru/jour/article/view/797<i>burkholderia</i>β-lactamasesmolecular typingpolymerase chain reaction
collection DOAJ
language Russian
format Article
sources DOAJ
author A. V. Romanova
I. B. Zakharova
V. S. Zamaraev
D. V. Viktorov
spellingShingle A. V. Romanova
I. B. Zakharova
V. S. Zamaraev
D. V. Viktorov
Design of Primers for Detection and Typing of β-Lactamase Genes from Pathogenic Species of <i>Burkholderia</i>
Проблемы особо опасных инфекций
<i>burkholderia</i>
β-lactamases
molecular typing
polymerase chain reaction
author_facet A. V. Romanova
I. B. Zakharova
V. S. Zamaraev
D. V. Viktorov
author_sort A. V. Romanova
title Design of Primers for Detection and Typing of β-Lactamase Genes from Pathogenic Species of <i>Burkholderia</i>
title_short Design of Primers for Detection and Typing of β-Lactamase Genes from Pathogenic Species of <i>Burkholderia</i>
title_full Design of Primers for Detection and Typing of β-Lactamase Genes from Pathogenic Species of <i>Burkholderia</i>
title_fullStr Design of Primers for Detection and Typing of β-Lactamase Genes from Pathogenic Species of <i>Burkholderia</i>
title_full_unstemmed Design of Primers for Detection and Typing of β-Lactamase Genes from Pathogenic Species of <i>Burkholderia</i>
title_sort design of primers for detection and typing of β-lactamase genes from pathogenic species of <i>burkholderia</i>
publisher Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
series Проблемы особо опасных инфекций
issn 0370-1069
2658-719X
publishDate 2012-04-01
description The set of oligonucleotide primers was designed to identify β-lactam-resitance determinats in isolates of pathogenic Burkholderia using PCR. Simultaneously identified was certain molecular class of detected β-lactamases. PCR with primers specific to metallo-β-lactamase (class B) and oxacillinase of D-ala carboxypeptidase family (class D of β-lactamase) gene sequences allowed to differentiate among pseudomallei group of Burkholderia species.
topic <i>burkholderia</i>
β-lactamases
molecular typing
polymerase chain reaction
url https://journal.microbe.ru/jour/article/view/797
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