Design of Primers for Detection and Typing of β-Lactamase Genes from Pathogenic Species of <i>Burkholderia</i>
The set of oligonucleotide primers was designed to identify β-lactam-resitance determinats in isolates of pathogenic Burkholderia using PCR. Simultaneously identified was certain molecular class of detected β-lactamases. PCR with primers specific to metallo-β-lactamase (class B) and oxacillinase of...
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Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2012-04-01
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Series: | Проблемы особо опасных инфекций |
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Online Access: | https://journal.microbe.ru/jour/article/view/797 |
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doaj-1f8e4ae8553d4183ad875ff37d117a952021-07-22T07:51:34ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2012-04-0102(112)596110.21055/0370-1069-2012-2(112)-59-61781Design of Primers for Detection and Typing of β-Lactamase Genes from Pathogenic Species of <i>Burkholderia</i>A. V. Romanova0I. B. Zakharova1V. S. Zamaraev2D. V. Viktorov3Volgograd Research Anti-Plague InstituteVolgograd Research Anti-Plague InstituteVolgograd Research Anti-Plague InstituteVolgograd Research Anti-Plague InstituteThe set of oligonucleotide primers was designed to identify β-lactam-resitance determinats in isolates of pathogenic Burkholderia using PCR. Simultaneously identified was certain molecular class of detected β-lactamases. PCR with primers specific to metallo-β-lactamase (class B) and oxacillinase of D-ala carboxypeptidase family (class D of β-lactamase) gene sequences allowed to differentiate among pseudomallei group of Burkholderia species.https://journal.microbe.ru/jour/article/view/797<i>burkholderia</i>β-lactamasesmolecular typingpolymerase chain reaction |
collection |
DOAJ |
language |
Russian |
format |
Article |
sources |
DOAJ |
author |
A. V. Romanova I. B. Zakharova V. S. Zamaraev D. V. Viktorov |
spellingShingle |
A. V. Romanova I. B. Zakharova V. S. Zamaraev D. V. Viktorov Design of Primers for Detection and Typing of β-Lactamase Genes from Pathogenic Species of <i>Burkholderia</i> Проблемы особо опасных инфекций <i>burkholderia</i> β-lactamases molecular typing polymerase chain reaction |
author_facet |
A. V. Romanova I. B. Zakharova V. S. Zamaraev D. V. Viktorov |
author_sort |
A. V. Romanova |
title |
Design of Primers for Detection and Typing of β-Lactamase Genes from Pathogenic Species of <i>Burkholderia</i> |
title_short |
Design of Primers for Detection and Typing of β-Lactamase Genes from Pathogenic Species of <i>Burkholderia</i> |
title_full |
Design of Primers for Detection and Typing of β-Lactamase Genes from Pathogenic Species of <i>Burkholderia</i> |
title_fullStr |
Design of Primers for Detection and Typing of β-Lactamase Genes from Pathogenic Species of <i>Burkholderia</i> |
title_full_unstemmed |
Design of Primers for Detection and Typing of β-Lactamase Genes from Pathogenic Species of <i>Burkholderia</i> |
title_sort |
design of primers for detection and typing of β-lactamase genes from pathogenic species of <i>burkholderia</i> |
publisher |
Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
series |
Проблемы особо опасных инфекций |
issn |
0370-1069 2658-719X |
publishDate |
2012-04-01 |
description |
The set of oligonucleotide primers was designed to identify β-lactam-resitance determinats in isolates of pathogenic Burkholderia using PCR. Simultaneously identified was certain molecular class of detected β-lactamases. PCR with primers specific to metallo-β-lactamase (class B) and oxacillinase of D-ala carboxypeptidase family (class D of β-lactamase) gene sequences allowed to differentiate among pseudomallei group of Burkholderia species. |
topic |
<i>burkholderia</i> β-lactamases molecular typing polymerase chain reaction |
url |
https://journal.microbe.ru/jour/article/view/797 |
work_keys_str_mv |
AT avromanova designofprimersfordetectionandtypingofblactamasegenesfrompathogenicspeciesofiburkholderiai AT ibzakharova designofprimersfordetectionandtypingofblactamasegenesfrompathogenicspeciesofiburkholderiai AT vszamaraev designofprimersfordetectionandtypingofblactamasegenesfrompathogenicspeciesofiburkholderiai AT dvviktorov designofprimersfordetectionandtypingofblactamasegenesfrompathogenicspeciesofiburkholderiai |
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1721291940246323200 |