Unlike twins: an NMR comparison of two α-synuclein polymorphs featuring different toxicity.
We structurally compare, using solid-state NMR, two different polymorphs of α-synuclein which, as established recently, display contrasting biochemical properties, toxicity, and tropism for cells. We show that both forms, which can each be produced as a pure polymorph, are greatly different in secon...
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doaj-1f8e8b5f4b604242897b2a53b763e1d72021-03-04T09:46:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0193e9065910.1371/journal.pone.0090659Unlike twins: an NMR comparison of two α-synuclein polymorphs featuring different toxicity.Julia GathLuc BoussetBirgit HabensteinRonald MelkiAnja BöckmannBeat H MeierWe structurally compare, using solid-state NMR, two different polymorphs of α-synuclein which, as established recently, display contrasting biochemical properties, toxicity, and tropism for cells. We show that both forms, which can each be produced as a pure polymorph, are greatly different in secondary structure. While β-sheets are the dominating secondary structure elements for both polymorphs, they are markedly divergent in terms of number of elements, as well as their distribution. We demonstrate that all identified β-sheets feature an in-register parallel stacking for both polymorphs. The two forms show a different molecular arrangement in the unit cell and distinct dynamic features, while sharing a highly flexible C-terminal domain. The use of reproducible, well-identified conditions for sample preparation and the recording of identical NMR experiments allows for a direct comparison of the results.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24599158/?tool=EBI |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Julia Gath Luc Bousset Birgit Habenstein Ronald Melki Anja Böckmann Beat H Meier |
spellingShingle |
Julia Gath Luc Bousset Birgit Habenstein Ronald Melki Anja Böckmann Beat H Meier Unlike twins: an NMR comparison of two α-synuclein polymorphs featuring different toxicity. PLoS ONE |
author_facet |
Julia Gath Luc Bousset Birgit Habenstein Ronald Melki Anja Böckmann Beat H Meier |
author_sort |
Julia Gath |
title |
Unlike twins: an NMR comparison of two α-synuclein polymorphs featuring different toxicity. |
title_short |
Unlike twins: an NMR comparison of two α-synuclein polymorphs featuring different toxicity. |
title_full |
Unlike twins: an NMR comparison of two α-synuclein polymorphs featuring different toxicity. |
title_fullStr |
Unlike twins: an NMR comparison of two α-synuclein polymorphs featuring different toxicity. |
title_full_unstemmed |
Unlike twins: an NMR comparison of two α-synuclein polymorphs featuring different toxicity. |
title_sort |
unlike twins: an nmr comparison of two α-synuclein polymorphs featuring different toxicity. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
We structurally compare, using solid-state NMR, two different polymorphs of α-synuclein which, as established recently, display contrasting biochemical properties, toxicity, and tropism for cells. We show that both forms, which can each be produced as a pure polymorph, are greatly different in secondary structure. While β-sheets are the dominating secondary structure elements for both polymorphs, they are markedly divergent in terms of number of elements, as well as their distribution. We demonstrate that all identified β-sheets feature an in-register parallel stacking for both polymorphs. The two forms show a different molecular arrangement in the unit cell and distinct dynamic features, while sharing a highly flexible C-terminal domain. The use of reproducible, well-identified conditions for sample preparation and the recording of identical NMR experiments allows for a direct comparison of the results. |
url |
https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24599158/?tool=EBI |
work_keys_str_mv |
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