Effects of Temperature and Water-Filtered Infrared-A Alone or in Combination on Healthy and Glyoxal-Stressed Fibroblast Cultures
Water-filtered infrared-A (wIRA) radiation has been described as supportive for tissue regeneration. We sought to investigate in detail the wIRA effect at different temperatures in 3T3 fibroblasts that were treated with glyoxal to induce formation of advanced glycation end products (AGEs). Nonirradi...
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Series: | Oxidative Medicine and Cellular Longevity |
Online Access: | http://dx.doi.org/10.1155/2012/274953 |
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doaj-1fb4169488544828b9c2090804e1940e2020-11-25T00:14:05ZengHindawi LimitedOxidative Medicine and Cellular Longevity1942-09001942-09942012-01-01201210.1155/2012/274953274953Effects of Temperature and Water-Filtered Infrared-A Alone or in Combination on Healthy and Glyoxal-Stressed Fibroblast CulturesLilla Knels0Monika Valtink1Jamlec De la Vega Marin2Gerald Steiner3Cora Roehlecke4Alexander Krueger5Richard H. W. Funk6Institute of Anatomy of the Faculty of Medicine, TU Dresden, 01307 Dresden, GermanyInstitute of Anatomy of the Faculty of Medicine, TU Dresden, 01307 Dresden, GermanyInstitute of Anatomy of the Faculty of Medicine, TU Dresden, 01307 Dresden, GermanyMedical Sensoring and Monitoring of the Faculty of Medicine Carl Gustav Carus, TU Dresden, 01307 Dresden, GermanyInstitute of Anatomy of the Faculty of Medicine, TU Dresden, 01307 Dresden, GermanyMedical Sensoring and Monitoring of the Faculty of Medicine Carl Gustav Carus, TU Dresden, 01307 Dresden, GermanyInstitute of Anatomy of the Faculty of Medicine, TU Dresden, 01307 Dresden, GermanyWater-filtered infrared-A (wIRA) radiation has been described as supportive for tissue regeneration. We sought to investigate in detail the wIRA effect at different temperatures in 3T3 fibroblasts that were treated with glyoxal to induce formation of advanced glycation end products (AGEs). Nonirradiated and nonglyoxal-treated cells served as controls. Experiments were carried out over a range of 37∘–45∘C with exact temperature monitoring to distinguish between temperature and wIRA effects. Metabolic activity was assessed by resazurin assay. Mitochondrial membrane potential was assessed by JC-1 vital staining. Apoptotic changes were determined by vital staining with annexin V and YO-PRO-1 and determination of subG1 DNA content. Temperature had a dominant effect overriding effects exerted by wIRA or glyoxal treatment. The number of apoptotic cells was significantly higher at 45∘C, while the percentage of healthy cells was significantly lower at 45∘C. WIRA irradiation itself or in combination with glyoxal treatment exerted no damaging effects on the fibroblasts at physiological (37∘–40∘C) or higher (42∘–45∘C) temperatures compared to untreated controls. Temperatures of 45∘C, which can occur during inappropriate application of infrared irradiation, damage cells even in the absence of wIRA or glyoxal application.http://dx.doi.org/10.1155/2012/274953 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lilla Knels Monika Valtink Jamlec De la Vega Marin Gerald Steiner Cora Roehlecke Alexander Krueger Richard H. W. Funk |
spellingShingle |
Lilla Knels Monika Valtink Jamlec De la Vega Marin Gerald Steiner Cora Roehlecke Alexander Krueger Richard H. W. Funk Effects of Temperature and Water-Filtered Infrared-A Alone or in Combination on Healthy and Glyoxal-Stressed Fibroblast Cultures Oxidative Medicine and Cellular Longevity |
author_facet |
Lilla Knels Monika Valtink Jamlec De la Vega Marin Gerald Steiner Cora Roehlecke Alexander Krueger Richard H. W. Funk |
author_sort |
Lilla Knels |
title |
Effects of Temperature and Water-Filtered Infrared-A Alone or in Combination on Healthy and Glyoxal-Stressed Fibroblast Cultures |
title_short |
Effects of Temperature and Water-Filtered Infrared-A Alone or in Combination on Healthy and Glyoxal-Stressed Fibroblast Cultures |
title_full |
Effects of Temperature and Water-Filtered Infrared-A Alone or in Combination on Healthy and Glyoxal-Stressed Fibroblast Cultures |
title_fullStr |
Effects of Temperature and Water-Filtered Infrared-A Alone or in Combination on Healthy and Glyoxal-Stressed Fibroblast Cultures |
title_full_unstemmed |
Effects of Temperature and Water-Filtered Infrared-A Alone or in Combination on Healthy and Glyoxal-Stressed Fibroblast Cultures |
title_sort |
effects of temperature and water-filtered infrared-a alone or in combination on healthy and glyoxal-stressed fibroblast cultures |
publisher |
Hindawi Limited |
series |
Oxidative Medicine and Cellular Longevity |
issn |
1942-0900 1942-0994 |
publishDate |
2012-01-01 |
description |
Water-filtered infrared-A (wIRA) radiation has been described as supportive for tissue regeneration. We sought to investigate in detail the wIRA effect at different temperatures in 3T3 fibroblasts that were treated with glyoxal to induce formation of advanced glycation end products (AGEs). Nonirradiated and nonglyoxal-treated cells served as controls. Experiments were carried out over a range of 37∘–45∘C with exact temperature monitoring to distinguish between temperature and wIRA effects. Metabolic activity was assessed by resazurin assay. Mitochondrial membrane potential was assessed by JC-1 vital staining. Apoptotic changes were determined by vital staining with annexin V and YO-PRO-1 and determination of subG1 DNA content. Temperature had a dominant effect overriding effects exerted by wIRA or glyoxal treatment. The number of apoptotic cells was significantly higher at 45∘C, while the percentage of healthy cells was significantly lower at 45∘C. WIRA irradiation itself or in combination with glyoxal treatment exerted no damaging effects on the fibroblasts at physiological (37∘–40∘C) or higher (42∘–45∘C) temperatures compared to untreated controls. Temperatures of 45∘C, which can occur during inappropriate application of infrared irradiation, damage cells even in the absence of wIRA or glyoxal application. |
url |
http://dx.doi.org/10.1155/2012/274953 |
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