Misdiagnosis of pulmonary tuberculosis and associated factors in peripheral laboratories: a retrospective study, Addis Ababa, Ethiopia
Abstract Objective Sputum smear microscopy reading errors are likely to result in failure to detect persons with infectious TB. This study was intended to review misdiagnosis of pulmonary TB and associated factors in peripheral laboratories. Results During the study period 1033 (10.5%) sputum smear...
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doaj-1fbd6d99b30a43b08d005650588361672020-11-25T02:22:58ZengBMCBMC Research Notes1756-05002018-05-011111710.1186/s13104-018-3414-6Misdiagnosis of pulmonary tuberculosis and associated factors in peripheral laboratories: a retrospective study, Addis Ababa, EthiopiaDaniel Melese Desalegn0Kumera Terfa Kitila1Hanna Mekonnen Balcha2Chalachew Sisay Gebeyehu3Yohannes W. Kidan4Kassayenew Amare5Daniel Dejene6Merone Seifu7Addis Zewdie8Abiyot Tenna9Tinsae Kidanemariam Hailu10Boja Dufera Taddese11Abrham Tesfaye Bika12Addis Ababa Public Health Research and Emergency Management Core Process, Addis Ababa City Administration Health BureauAddis Ababa Public Health Research and Emergency Management Core Process, Addis Ababa City Administration Health BureauAddis Ababa Public Health Research and Emergency Management Core Process, Addis Ababa City Administration Health BureauAddis Ababa Public Health Research and Emergency Management Core Process, Addis Ababa City Administration Health BureauAddis Ababa Public Health Research and Emergency Management Core Process, Addis Ababa City Administration Health BureauAddis Ababa Public Health Research and Emergency Management Core Process, Addis Ababa City Administration Health BureauAddis Ababa Public Health Research and Emergency Management Core Process, Addis Ababa City Administration Health BureauAddis Ababa Public Health Research and Emergency Management Core Process, Addis Ababa City Administration Health BureauAddis Ababa Public Health Research and Emergency Management Core Process, Addis Ababa City Administration Health BureauAddis Ababa Public Health Research and Emergency Management Core Process, Addis Ababa City Administration Health BureauAddis Ababa Public Health Research and Emergency Management Core Process, Addis Ababa City Administration Health BureauAddis Ababa Public Health Research and Emergency Management Core Process, Addis Ababa City Administration Health BureauAddis Ababa Public Health Research and Emergency Management Core Process, Addis Ababa City Administration Health BureauAbstract Objective Sputum smear microscopy reading errors are likely to result in failure to detect persons with infectious TB. This study was intended to review misdiagnosis of pulmonary TB and associated factors in peripheral laboratories. Results During the study period 1033 (10.5%) sputum smear positive and 8783 (89.5%) smear negative slides were reported by peripheral laboratories. The slides were re-read by the central referral laboratories (CRLs) as the reference standard reading. Of 1033 positive slides reported by peripheral laboratories, 25 (2.4%) were false positive. Out of 8783 smear negative slides reported by peripheral laboratories, 35 (0.4%) were false negative. The sensitivity, specificity, positive predictive value and negative predictive value of peripheral laboratories were 96.64, 99.72, 97.58, and 99.61% respectively. The peripheral laboratories and CRLs have an observed agreement (Po) of 0.9939. Of 135 peripheral laboratories, 93 (68.9%) read negative and positive slides correctly, 49 (36.3%) did not have lens cleaning tissue papers, 11 (8.1%) lacked frosted slides, and 14 (10.4%) had shortage of reagents. As conclusions, the peripheral laboratories and CRLs had high agreement for sputum smear microscopy reading. However, a few TB cases were misdiagnosed despite having the disease; these individuals might continue to spread the infection in the community.http://link.springer.com/article/10.1186/s13104-018-3414-6External quality assessmentZN sputum smear microscopyAddis Ababa |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Daniel Melese Desalegn Kumera Terfa Kitila Hanna Mekonnen Balcha Chalachew Sisay Gebeyehu Yohannes W. Kidan Kassayenew Amare Daniel Dejene Merone Seifu Addis Zewdie Abiyot Tenna Tinsae Kidanemariam Hailu Boja Dufera Taddese Abrham Tesfaye Bika |
spellingShingle |
Daniel Melese Desalegn Kumera Terfa Kitila Hanna Mekonnen Balcha Chalachew Sisay Gebeyehu Yohannes W. Kidan Kassayenew Amare Daniel Dejene Merone Seifu Addis Zewdie Abiyot Tenna Tinsae Kidanemariam Hailu Boja Dufera Taddese Abrham Tesfaye Bika Misdiagnosis of pulmonary tuberculosis and associated factors in peripheral laboratories: a retrospective study, Addis Ababa, Ethiopia BMC Research Notes External quality assessment ZN sputum smear microscopy Addis Ababa |
author_facet |
Daniel Melese Desalegn Kumera Terfa Kitila Hanna Mekonnen Balcha Chalachew Sisay Gebeyehu Yohannes W. Kidan Kassayenew Amare Daniel Dejene Merone Seifu Addis Zewdie Abiyot Tenna Tinsae Kidanemariam Hailu Boja Dufera Taddese Abrham Tesfaye Bika |
author_sort |
Daniel Melese Desalegn |
title |
Misdiagnosis of pulmonary tuberculosis and associated factors in peripheral laboratories: a retrospective study, Addis Ababa, Ethiopia |
title_short |
Misdiagnosis of pulmonary tuberculosis and associated factors in peripheral laboratories: a retrospective study, Addis Ababa, Ethiopia |
title_full |
Misdiagnosis of pulmonary tuberculosis and associated factors in peripheral laboratories: a retrospective study, Addis Ababa, Ethiopia |
title_fullStr |
Misdiagnosis of pulmonary tuberculosis and associated factors in peripheral laboratories: a retrospective study, Addis Ababa, Ethiopia |
title_full_unstemmed |
Misdiagnosis of pulmonary tuberculosis and associated factors in peripheral laboratories: a retrospective study, Addis Ababa, Ethiopia |
title_sort |
misdiagnosis of pulmonary tuberculosis and associated factors in peripheral laboratories: a retrospective study, addis ababa, ethiopia |
publisher |
BMC |
series |
BMC Research Notes |
issn |
1756-0500 |
publishDate |
2018-05-01 |
description |
Abstract Objective Sputum smear microscopy reading errors are likely to result in failure to detect persons with infectious TB. This study was intended to review misdiagnosis of pulmonary TB and associated factors in peripheral laboratories. Results During the study period 1033 (10.5%) sputum smear positive and 8783 (89.5%) smear negative slides were reported by peripheral laboratories. The slides were re-read by the central referral laboratories (CRLs) as the reference standard reading. Of 1033 positive slides reported by peripheral laboratories, 25 (2.4%) were false positive. Out of 8783 smear negative slides reported by peripheral laboratories, 35 (0.4%) were false negative. The sensitivity, specificity, positive predictive value and negative predictive value of peripheral laboratories were 96.64, 99.72, 97.58, and 99.61% respectively. The peripheral laboratories and CRLs have an observed agreement (Po) of 0.9939. Of 135 peripheral laboratories, 93 (68.9%) read negative and positive slides correctly, 49 (36.3%) did not have lens cleaning tissue papers, 11 (8.1%) lacked frosted slides, and 14 (10.4%) had shortage of reagents. As conclusions, the peripheral laboratories and CRLs had high agreement for sputum smear microscopy reading. However, a few TB cases were misdiagnosed despite having the disease; these individuals might continue to spread the infection in the community. |
topic |
External quality assessment ZN sputum smear microscopy Addis Ababa |
url |
http://link.springer.com/article/10.1186/s13104-018-3414-6 |
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