Identification of characteristic compounds of moderate volatility in breast cancer cell lines.
In this study, we were challenging to identify characteristic compounds in breast cancer cell lines. GC analysis of extracts from the culture media of breast cancer cell lines (MCF-7, SK-BR-3, and YMB-1) using a solid-phase Porapak Q extraction revealed that two compounds of moderate volatility, 1-h...
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doaj-1fc4ac9cdcb146e9b85394f7d12d98b82021-03-03T21:53:15ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01156e023544210.1371/journal.pone.0235442Identification of characteristic compounds of moderate volatility in breast cancer cell lines.Mitsuru TanakaChung HsuanMasataka OekiWeilin ShenAsuka GodaYusuke TaharaTakeshi OnoderaKeisuke SanematsuTomotsugu RikitakeEiji OkiYuzo NinomiyaRintaro KurebayashiHideto SonodaYoshihiko MaeharaKiyoshi TokoToshiro MatsuiIn this study, we were challenging to identify characteristic compounds in breast cancer cell lines. GC analysis of extracts from the culture media of breast cancer cell lines (MCF-7, SK-BR-3, and YMB-1) using a solid-phase Porapak Q extraction revealed that two compounds of moderate volatility, 1-hexadecanol and 5-(Z)-dodecenoic acid, were detected with markedly higher amount than those in the medium of fibroblast cell line (KMST-6). Furthermore, LC-TOF/MS analysis of the extracts clarified that in addition to the above two fatty acids, the amounts of five unsaturated fatty acids [decenoic acid (C10:1), decadienoic acid (C10:2), 5-(Z)-dodecenoic acid (C12:1), 5-(Z)-tetradecenoic acid (C14:1), and tetradecadienoic acid (C14:2)] in MCF-7 medium were higher than those in medium of KMST-6. Interestingly, H2O2-oxidation of 5-(Z)-dodecenoic acid and 5-(Z)-tetradecenoic acid produced volatile aldehydes that were reported as specific volatiles in breath from various cancer patients, such as heptanal, octanal, nonanal, decanal, 2-(E)-nonenal, and 2-(E)-octenal. Thus, we concluded that these identified compounds over-produced in breast cancer cells in this study could serve as potential precursors producing reported cancer-specific volatiles.https://doi.org/10.1371/journal.pone.0235442 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mitsuru Tanaka Chung Hsuan Masataka Oeki Weilin Shen Asuka Goda Yusuke Tahara Takeshi Onodera Keisuke Sanematsu Tomotsugu Rikitake Eiji Oki Yuzo Ninomiya Rintaro Kurebayashi Hideto Sonoda Yoshihiko Maehara Kiyoshi Toko Toshiro Matsui |
spellingShingle |
Mitsuru Tanaka Chung Hsuan Masataka Oeki Weilin Shen Asuka Goda Yusuke Tahara Takeshi Onodera Keisuke Sanematsu Tomotsugu Rikitake Eiji Oki Yuzo Ninomiya Rintaro Kurebayashi Hideto Sonoda Yoshihiko Maehara Kiyoshi Toko Toshiro Matsui Identification of characteristic compounds of moderate volatility in breast cancer cell lines. PLoS ONE |
author_facet |
Mitsuru Tanaka Chung Hsuan Masataka Oeki Weilin Shen Asuka Goda Yusuke Tahara Takeshi Onodera Keisuke Sanematsu Tomotsugu Rikitake Eiji Oki Yuzo Ninomiya Rintaro Kurebayashi Hideto Sonoda Yoshihiko Maehara Kiyoshi Toko Toshiro Matsui |
author_sort |
Mitsuru Tanaka |
title |
Identification of characteristic compounds of moderate volatility in breast cancer cell lines. |
title_short |
Identification of characteristic compounds of moderate volatility in breast cancer cell lines. |
title_full |
Identification of characteristic compounds of moderate volatility in breast cancer cell lines. |
title_fullStr |
Identification of characteristic compounds of moderate volatility in breast cancer cell lines. |
title_full_unstemmed |
Identification of characteristic compounds of moderate volatility in breast cancer cell lines. |
title_sort |
identification of characteristic compounds of moderate volatility in breast cancer cell lines. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2020-01-01 |
description |
In this study, we were challenging to identify characteristic compounds in breast cancer cell lines. GC analysis of extracts from the culture media of breast cancer cell lines (MCF-7, SK-BR-3, and YMB-1) using a solid-phase Porapak Q extraction revealed that two compounds of moderate volatility, 1-hexadecanol and 5-(Z)-dodecenoic acid, were detected with markedly higher amount than those in the medium of fibroblast cell line (KMST-6). Furthermore, LC-TOF/MS analysis of the extracts clarified that in addition to the above two fatty acids, the amounts of five unsaturated fatty acids [decenoic acid (C10:1), decadienoic acid (C10:2), 5-(Z)-dodecenoic acid (C12:1), 5-(Z)-tetradecenoic acid (C14:1), and tetradecadienoic acid (C14:2)] in MCF-7 medium were higher than those in medium of KMST-6. Interestingly, H2O2-oxidation of 5-(Z)-dodecenoic acid and 5-(Z)-tetradecenoic acid produced volatile aldehydes that were reported as specific volatiles in breath from various cancer patients, such as heptanal, octanal, nonanal, decanal, 2-(E)-nonenal, and 2-(E)-octenal. Thus, we concluded that these identified compounds over-produced in breast cancer cells in this study could serve as potential precursors producing reported cancer-specific volatiles. |
url |
https://doi.org/10.1371/journal.pone.0235442 |
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