EVALUATION OF THE EFFECT OF SPUTUM STORAGE CONDITIONS ON THE VITAL PROPERTIES OF TUBERCULOUS MYCOBACTERIA

• Main Authors: Yu. D. Rodionova, O. A. Gusyakova, A. V. Lyamin, E. A. Borodulina, A. V. Kozlov
• Format: Article
• Language: Russian
• Published: NEW TERRA Publishing House 2017-01-01
• Series: Tuberkulez i Bolezni Lëgkih
• Subjects: sputum storage conditions; growth properties; tuberculous mycobacteria; nutritive medium; laboratory diagnostics
• Online Access: https://www.tibl-journal.com/jour/article/view/965

Goal of the study: to enhance efficiency of laboratory diagnostics of tuberculous infection through investigating the effect of various storage conditions of samples on the growth properties of Tuberculosis complex mycobacteria.Materials and Methods. 2058 samples of sputum collected by coughing were examined. All sputum samples were aliquoted into 5 parts stored under various conditions: in the fridge under +7°С for 2 hours; frozen for 7 days; by room indoor temperature for 48-72 hours; with the use preservative 10% solution of triple-substituted natrium phosphate for 48 hours; in the fridge under +7°С for 2 hours with consequent treatment by 1% solution of N-acetyl-L-cysteine.Results. The most optimal sputum storage conditions are freezing by -20°С, providing maximum safety of mycobacteria and minimum contamination of the samples with foreign bacteria. Sputum storage by indoor room temperature for 2-3 days reduces the number of positive results of cultures on nutritive media. Using 10% solution of triple-substituted natrium phosphate provides high positive results of bacterioscopic and cultural testing techniques. Time prolongation for biomaterials storage in the preservative solution for more than 72 hours results in the death tuberculous mycobacteria.