Blood Contamination in Saliva: Impact on the Measurement of Salivary Oxidative Stress Markers
Salivary oxidative stress markers represent a promising tool for monitoring of oral diseases. Saliva can often be contaminated by blood, especially in patients with periodontitis. The aim of our study was to examine the impact of blood contamination on the measurement of salivary oxidative stress ma...
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Series: | Disease Markers |
Online Access: | http://dx.doi.org/10.1155/2015/479251 |
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doaj-1fc8bf001a0f4f26896f25bef002011a2020-11-24T23:24:45ZengHindawi LimitedDisease Markers0278-02401875-86302015-01-01201510.1155/2015/479251479251Blood Contamination in Saliva: Impact on the Measurement of Salivary Oxidative Stress MarkersNatália Kamodyová0Lenka Baňasová1Katarína Janšáková2Ivana Koborová3Ľubomíra Tóthová4Peter Stanko5Peter Celec6Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Sasinkova 4, 811 08 Bratislava, SlovakiaDepartment of Stomatology and Maxillofacial Surgery, Comenius University, Heydukova 10, 812 50 Bratislava, SlovakiaInstitute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Sasinkova 4, 811 08 Bratislava, SlovakiaInstitute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Sasinkova 4, 811 08 Bratislava, SlovakiaInstitute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Sasinkova 4, 811 08 Bratislava, SlovakiaDepartment of Stomatology and Maxillofacial Surgery, Comenius University, Heydukova 10, 812 50 Bratislava, SlovakiaInstitute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Sasinkova 4, 811 08 Bratislava, SlovakiaSalivary oxidative stress markers represent a promising tool for monitoring of oral diseases. Saliva can often be contaminated by blood, especially in patients with periodontitis. The aim of our study was to examine the impact of blood contamination on the measurement of salivary oxidative stress markers. Saliva samples were collected from 10 healthy volunteers and were artificially contaminated with blood (final concentration 0.001–10%). Next, saliva was collected from 12 gingivitis and 10 control patients before and after dental hygiene treatment. Markers of oxidative stress were measured in all collected saliva samples. Advanced oxidation protein products (AOPP), advanced glycation end products (AGEs), and antioxidant status were changed in 1% blood-contaminated saliva. Salivary AOPP were increased in control and patients after dental treatment (by 45.7% and 34.1%, p<0.01). Salivary AGEs were decreased in patients after microinjury (by 69.3%, p<0.001). Salivary antioxidant status markers were decreased in both control and patients after dental treatment (p<0.05 and p<0.01). One % blood contamination biased concentrations of salivary oxidative stress markers. Saliva samples with 1% blood contamination are visibly discolored and can be excluded from analyses without any specific biochemic detection of blood constituents. Salivary markers of oxidative stress were significantly altered in blood-contaminated saliva in control and patients with gingivitis after dental hygiene treatment.http://dx.doi.org/10.1155/2015/479251 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Natália Kamodyová Lenka Baňasová Katarína Janšáková Ivana Koborová Ľubomíra Tóthová Peter Stanko Peter Celec |
spellingShingle |
Natália Kamodyová Lenka Baňasová Katarína Janšáková Ivana Koborová Ľubomíra Tóthová Peter Stanko Peter Celec Blood Contamination in Saliva: Impact on the Measurement of Salivary Oxidative Stress Markers Disease Markers |
author_facet |
Natália Kamodyová Lenka Baňasová Katarína Janšáková Ivana Koborová Ľubomíra Tóthová Peter Stanko Peter Celec |
author_sort |
Natália Kamodyová |
title |
Blood Contamination in Saliva: Impact on the Measurement of Salivary Oxidative Stress Markers |
title_short |
Blood Contamination in Saliva: Impact on the Measurement of Salivary Oxidative Stress Markers |
title_full |
Blood Contamination in Saliva: Impact on the Measurement of Salivary Oxidative Stress Markers |
title_fullStr |
Blood Contamination in Saliva: Impact on the Measurement of Salivary Oxidative Stress Markers |
title_full_unstemmed |
Blood Contamination in Saliva: Impact on the Measurement of Salivary Oxidative Stress Markers |
title_sort |
blood contamination in saliva: impact on the measurement of salivary oxidative stress markers |
publisher |
Hindawi Limited |
series |
Disease Markers |
issn |
0278-0240 1875-8630 |
publishDate |
2015-01-01 |
description |
Salivary oxidative stress markers represent a promising tool for monitoring of oral diseases. Saliva can often be contaminated by blood, especially in patients with periodontitis. The aim of our study was to examine the impact of blood contamination on the measurement of salivary oxidative stress markers. Saliva samples were collected from 10 healthy volunteers and were artificially contaminated with blood (final concentration 0.001–10%). Next, saliva was collected from 12 gingivitis and 10 control patients before and after dental hygiene treatment. Markers of oxidative stress were measured in all collected saliva samples. Advanced oxidation protein products (AOPP), advanced glycation end products (AGEs), and antioxidant status were changed in 1% blood-contaminated saliva. Salivary AOPP were increased in control and patients after dental treatment (by 45.7% and 34.1%, p<0.01). Salivary AGEs were decreased in patients after microinjury (by 69.3%, p<0.001). Salivary antioxidant status markers were decreased in both control and patients after dental treatment (p<0.05 and p<0.01). One % blood contamination biased concentrations of salivary oxidative stress markers. Saliva samples with 1% blood contamination are visibly discolored and can be excluded from analyses without any specific biochemic detection of blood constituents. Salivary markers of oxidative stress were significantly altered in blood-contaminated saliva in control and patients with gingivitis after dental hygiene treatment. |
url |
http://dx.doi.org/10.1155/2015/479251 |
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