Using Simple Sequence Repeats in 9 <i>Brassica</i> Complex Species to Assess Hypertrophic Curd Induction
Five Simple Sequence Repeats (SSRs) were used to assess the relationship between inflorescence characteristics and their allelic variation in 53 <i>Brassica oleracea</i> and <i>Brassica</i> wild relatives (<i>n</i> = 9). Curd morphometric traits, such as weight (C...
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doaj-1fcf54766cc749b28ecacac5f8de83562021-07-23T13:25:56ZengMDPI AGAgriculture2077-04722021-07-011162262210.3390/agriculture11070622Using Simple Sequence Repeats in 9 <i>Brassica</i> Complex Species to Assess Hypertrophic Curd InductionSimone Treccarichi0Cornelia Di Gaetano1Fulvio Di Stefano2Mauro Gasparini3Ferdinando Branca4Food and Environment (Di3A), Department of Agriculture, University of Catania, 95131 Catania, ItalyDepartment of Medical Science, University of Turin, 10126 Turin, ItalyDepartment of Mathematical Science, Politecnico di Torino, 10126 Turin, ItalyDepartment of Mathematical Science, Politecnico di Torino, 10126 Turin, ItalyFood and Environment (Di3A), Department of Agriculture, University of Catania, 95131 Catania, ItalyFive Simple Sequence Repeats (SSRs) were used to assess the relationship between inflorescence characteristics and their allelic variation in 53 <i>Brassica oleracea</i> and <i>Brassica</i> wild relatives (<i>n</i> = 9). Curd morphometric traits, such as weight (CW), height (CH), diameter (CD1), shape (CS) inflorescence curvature angle (CA), and its curd stem diameter (CD2), were measured. The aim of the work was to analyze the relationships among the allelic patterns of the SSRs primers utilized, and their status of homo or heterozygosity registered at each locus, as well as the inflorescence morphometric traits in order to individuate genomic regions stimulating the hypertrophy of this reproductive organ. The relationships found explain the diversity among <i>B</i>. <i>oleracea</i> complex species (<i>n</i> = 9) for the inflorescence size and structure, allowing important time reduction during the breeding process by crossing wild species, transferring useful resistance, and organoleptic and nutraceutical traits. The five SSRs loci were BoABI1, BoAP1, BoPLD1, BoTHL1, and PBCGSSRBo39. According to the allelic variation ascertained, we evaluated the heterozygosity index (H) for each SSR above cited. The results showed a significant interaction between the H index of the BoPLD1 gene and the inflorescence characteristics, summarized by the First Principal Component (PC1) (<i>p</i>-value = 0.0244); we ascertained a negative correlation between the H index and inflorescence characteristics, namely CW, CH, CD1, CD2, CA. The homozygosity BoPLD1 alelles, indicated by the H index, affect the inflorescence characteristics and broccoli and cauliflower yields.https://www.mdpi.com/2077-0472/11/7/622<i>Brassica</i> complex speciesMADH–box genesSSRs assayheterozygosity indexallelic variancecurd morphometric traits |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Simone Treccarichi Cornelia Di Gaetano Fulvio Di Stefano Mauro Gasparini Ferdinando Branca |
spellingShingle |
Simone Treccarichi Cornelia Di Gaetano Fulvio Di Stefano Mauro Gasparini Ferdinando Branca Using Simple Sequence Repeats in 9 <i>Brassica</i> Complex Species to Assess Hypertrophic Curd Induction Agriculture <i>Brassica</i> complex species MADH–box genes SSRs assay heterozygosity index allelic variance curd morphometric traits |
author_facet |
Simone Treccarichi Cornelia Di Gaetano Fulvio Di Stefano Mauro Gasparini Ferdinando Branca |
author_sort |
Simone Treccarichi |
title |
Using Simple Sequence Repeats in 9 <i>Brassica</i> Complex Species to Assess Hypertrophic Curd Induction |
title_short |
Using Simple Sequence Repeats in 9 <i>Brassica</i> Complex Species to Assess Hypertrophic Curd Induction |
title_full |
Using Simple Sequence Repeats in 9 <i>Brassica</i> Complex Species to Assess Hypertrophic Curd Induction |
title_fullStr |
Using Simple Sequence Repeats in 9 <i>Brassica</i> Complex Species to Assess Hypertrophic Curd Induction |
title_full_unstemmed |
Using Simple Sequence Repeats in 9 <i>Brassica</i> Complex Species to Assess Hypertrophic Curd Induction |
title_sort |
using simple sequence repeats in 9 <i>brassica</i> complex species to assess hypertrophic curd induction |
publisher |
MDPI AG |
series |
Agriculture |
issn |
2077-0472 |
publishDate |
2021-07-01 |
description |
Five Simple Sequence Repeats (SSRs) were used to assess the relationship between inflorescence characteristics and their allelic variation in 53 <i>Brassica oleracea</i> and <i>Brassica</i> wild relatives (<i>n</i> = 9). Curd morphometric traits, such as weight (CW), height (CH), diameter (CD1), shape (CS) inflorescence curvature angle (CA), and its curd stem diameter (CD2), were measured. The aim of the work was to analyze the relationships among the allelic patterns of the SSRs primers utilized, and their status of homo or heterozygosity registered at each locus, as well as the inflorescence morphometric traits in order to individuate genomic regions stimulating the hypertrophy of this reproductive organ. The relationships found explain the diversity among <i>B</i>. <i>oleracea</i> complex species (<i>n</i> = 9) for the inflorescence size and structure, allowing important time reduction during the breeding process by crossing wild species, transferring useful resistance, and organoleptic and nutraceutical traits. The five SSRs loci were BoABI1, BoAP1, BoPLD1, BoTHL1, and PBCGSSRBo39. According to the allelic variation ascertained, we evaluated the heterozygosity index (H) for each SSR above cited. The results showed a significant interaction between the H index of the BoPLD1 gene and the inflorescence characteristics, summarized by the First Principal Component (PC1) (<i>p</i>-value = 0.0244); we ascertained a negative correlation between the H index and inflorescence characteristics, namely CW, CH, CD1, CD2, CA. The homozygosity BoPLD1 alelles, indicated by the H index, affect the inflorescence characteristics and broccoli and cauliflower yields. |
topic |
<i>Brassica</i> complex species MADH–box genes SSRs assay heterozygosity index allelic variance curd morphometric traits |
url |
https://www.mdpi.com/2077-0472/11/7/622 |
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