Thermomyces lanuginosus CBS 395.62/b Strain as Rich Source of α-Galactosidase Enzyme

• Main Authors: Quang D. Nguyen, Erika Bujna, Krisztina Takács, Magdolna Kovács, Ágoston Hoschke, Judit M. Rezessy-Szabó
• Format: Article
• Language: English
• Published: University of Zagreb 2003-01-01
• Series: Food Technology and Biotechnology
• Subjects: α-galactosidase; fermentation; enzyme production; Thermomyces lanuginosus
• Online Access: http://hrcak.srce.hr/file/163496
• ISSN: 1330-9862; 1334-2606

Seventeen Thermomyces lanuginosus strains, cultivated on raffinose and sucrose, were ranked on the basis of α-galactosidase activities. T. lanuginosus CBS 395.62/b strain showed the highest α-galactosidase activity on both investigated carbohydrates. Several carbon sources were tested as potential inducers for the α-galactosidase synthesis. On melibiose substrate α-galactosidase activity was higher in the intracellular fraction than in the filtrate of the fermentation broth, although both values were very low and did not reach the value of 1 U/mL. Raffinose, sucrose and Lactosucrose® proved to be inducers for α-galactosidase production. The highest titer (about 30 U/mL) was achieved on 1 % sucrose and 0.45 % ammonium acetate. The optimum sucrose and ammonium acetate concentrations, at which about 90 U/mL α-galactosidase activity was reached during an 8-day fermentation, were 3 and 0.9 %, respectively.