IgG4 and IgE co-positive group found in idiopathic orbital inflammatory disease

AIM: To reveal the cytokines involved in idiopathic orbital inflammatory disease (IOID) and the relationship between Th17 cells, IgE and IOID pathogenesis. METHODS: Whole blood samples were processed immediately after collection and serological IgG4, IgG, and IgE antibodies were tested using ELISA....

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Main Authors: Peng-Xiang Zhao, Yao Mawulikplimi Adzavon, Jian-Min Ma, Lei Shang, Dan-Ying Chen, Fei Xie, Meng-Yu Liu, Xin Zhang, Bao-Bei Lyu, Ming-Zi Zhang, Lin-Qi Yang, Xue-Mei Ma
Format: Article
Language:English
Published: Press of International Journal of Ophthalmology (IJO PRESS) 2018-01-01
Series:International Journal of Ophthalmology
Subjects:
42
IgE
Online Access:http://www.ijo.cn/en_publish/2018/1/20180107.pdf
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spelling doaj-1ffefd4a4d484f8b90eb98b426ab24f92020-11-25T00:07:09ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982018-01-01111364210.18240/ijo.2018.01.07IgG4 and IgE co-positive group found in idiopathic orbital inflammatory diseasePeng-Xiang Zhao0Yao Mawulikplimi Adzavon1Jian-Min Ma2Lei Shang3Dan-Ying Chen4Fei Xie5Meng-Yu Liu6Xin Zhang7Bao-Bei Lyu8Ming-Zi Zhang9Lin-Qi Yang10Xue-Mei Ma11College of Life Science and Bio-engineering, Beijing University of Technology, Beijing 100124, ChinaCollege of Life Science and Bio-engineering, Beijing University of Technology, Beijing 100124, ChinaBeijing Ophthalmology & Vision Science Key Lab, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, ChinaCollege of Life Science and Bio-engineering, Beijing University of Technology, Beijing 100124, ChinaInstitute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, ChinaCollege of Life Science and Bio-engineering, Beijing University of Technology, Beijing 100124, ChinaCollege of Life Science and Bio-engineering, Beijing University of Technology, Beijing 100124, ChinaCollege of Life Science and Bio-engineering, Beijing University of Technology, Beijing 100124, ChinaCollege of Life Science and Bio-engineering, Beijing University of Technology, Beijing 100124, ChinaDepartment of Plastic Surgery, Peking Union Medical College Hospital, Beijing 100730, ChinaCollege of Life Science and Bio-engineering, Beijing University of Technology, Beijing 100124, ChinaCollege of Life Science and Bio-engineering, Beijing University of Technology, Beijing 100124, ChinaAIM: To reveal the cytokines involved in idiopathic orbital inflammatory disease (IOID) and the relationship between Th17 cells, IgE and IOID pathogenesis. METHODS: Whole blood samples were processed immediately after collection and serological IgG4, IgG, and IgE antibodies were tested using ELISA. IOID and orbital cavernous hemangioma (CH) tissue samples underwent Bio-Plex multiplex cytokine detection. Hematoxylin-Eosin (HE) staining of all paraffin samples suggested the histological features of IOIDs, and expressions of IgG4 and IL-17A in affected tissues were detected by immunohistochemistry. RESULTS: Among 40 IOID plasma samples, 52.5% (21/40) were positive for IgG4 and 25% (10/40) were positive for IgE. Overlapped IgG4 or IgE positive samples accounted for 22.5% (9/40). Therefore, IOID samples were separated into three groups. The IgE+/IgG4+ group had a relevantly lower level of pro-inflammatory cytokine expression. IL-4 (Th2 cell related), IL-10 and TGF-β1 (Treg cell immunity related) were elevated in all three groups. Some of the Th17 cell related cytokines (i.e. IL-17A/F, IL-25, IL-23, and IL-33) displayed higher expression levels in the IgE-/IgG4- group compared to the other two groups. CONCLUSION: We discovered an IgG4-IgE co-positive group as well as Th17 cell immune involvement in IgG4-IgE co-negative subgtroup in IOID for the first time. The pathogenesis of IOID could differ from different subgroups according to the IgG4 and IgE detection. Therefore, we recommend that, Treatment stratagy should be made according to the clinical assessment of IgG4-IgE and Th17 profile detection.http://www.ijo.cn/en_publish/2018/1/20180107.pdf42idiopathic orbital inflammatory diseaseIgEIgG4Th17
collection DOAJ
language English
format Article
sources DOAJ
author Peng-Xiang Zhao
Yao Mawulikplimi Adzavon
Jian-Min Ma
Lei Shang
Dan-Ying Chen
Fei Xie
Meng-Yu Liu
Xin Zhang
Bao-Bei Lyu
Ming-Zi Zhang
Lin-Qi Yang
Xue-Mei Ma
spellingShingle Peng-Xiang Zhao
Yao Mawulikplimi Adzavon
Jian-Min Ma
Lei Shang
Dan-Ying Chen
Fei Xie
Meng-Yu Liu
Xin Zhang
Bao-Bei Lyu
Ming-Zi Zhang
Lin-Qi Yang
Xue-Mei Ma
IgG4 and IgE co-positive group found in idiopathic orbital inflammatory disease
International Journal of Ophthalmology
42
idiopathic orbital inflammatory disease
IgE
IgG4
Th17
author_facet Peng-Xiang Zhao
Yao Mawulikplimi Adzavon
Jian-Min Ma
Lei Shang
Dan-Ying Chen
Fei Xie
Meng-Yu Liu
Xin Zhang
Bao-Bei Lyu
Ming-Zi Zhang
Lin-Qi Yang
Xue-Mei Ma
author_sort Peng-Xiang Zhao
title IgG4 and IgE co-positive group found in idiopathic orbital inflammatory disease
title_short IgG4 and IgE co-positive group found in idiopathic orbital inflammatory disease
title_full IgG4 and IgE co-positive group found in idiopathic orbital inflammatory disease
title_fullStr IgG4 and IgE co-positive group found in idiopathic orbital inflammatory disease
title_full_unstemmed IgG4 and IgE co-positive group found in idiopathic orbital inflammatory disease
title_sort igg4 and ige co-positive group found in idiopathic orbital inflammatory disease
publisher Press of International Journal of Ophthalmology (IJO PRESS)
series International Journal of Ophthalmology
issn 2222-3959
2227-4898
publishDate 2018-01-01
description AIM: To reveal the cytokines involved in idiopathic orbital inflammatory disease (IOID) and the relationship between Th17 cells, IgE and IOID pathogenesis. METHODS: Whole blood samples were processed immediately after collection and serological IgG4, IgG, and IgE antibodies were tested using ELISA. IOID and orbital cavernous hemangioma (CH) tissue samples underwent Bio-Plex multiplex cytokine detection. Hematoxylin-Eosin (HE) staining of all paraffin samples suggested the histological features of IOIDs, and expressions of IgG4 and IL-17A in affected tissues were detected by immunohistochemistry. RESULTS: Among 40 IOID plasma samples, 52.5% (21/40) were positive for IgG4 and 25% (10/40) were positive for IgE. Overlapped IgG4 or IgE positive samples accounted for 22.5% (9/40). Therefore, IOID samples were separated into three groups. The IgE+/IgG4+ group had a relevantly lower level of pro-inflammatory cytokine expression. IL-4 (Th2 cell related), IL-10 and TGF-β1 (Treg cell immunity related) were elevated in all three groups. Some of the Th17 cell related cytokines (i.e. IL-17A/F, IL-25, IL-23, and IL-33) displayed higher expression levels in the IgE-/IgG4- group compared to the other two groups. CONCLUSION: We discovered an IgG4-IgE co-positive group as well as Th17 cell immune involvement in IgG4-IgE co-negative subgtroup in IOID for the first time. The pathogenesis of IOID could differ from different subgroups according to the IgG4 and IgE detection. Therefore, we recommend that, Treatment stratagy should be made according to the clinical assessment of IgG4-IgE and Th17 profile detection.
topic 42
idiopathic orbital inflammatory disease
IgE
IgG4
Th17
url http://www.ijo.cn/en_publish/2018/1/20180107.pdf
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