Investigating organic multilayers by spectroscopic ellipsometry: specific and non-specific interactions of polyhistidine with NTA self-assembled monolayers

Investigating organic multilayers by spectroscopic ellipsometry: specific and non-specific interactions of polyhistidine with NTA self-assembled monolayers

Background: A versatile strategy for protein–surface coupling in biochips exploits the affinity for polyhistidine of the nitrilotriacetic acid (NTA) group loaded with Ni(II). Methods based on optical reflectivity measurements such as spectroscopic ellipsometry (SE) allow for label-free, non-invasive...

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Bibliographic Details
Main Authors: Ilaria Solano, Pietro Parisse, Ornella Cavalleri, Federico Gramazio, Loredana Casalis, Maurizio Canepa
Format: Article
Language:English
Published: Beilstein-Institut 2016-04-01
Series:Beilstein Journal of Nanotechnology
Subjects:
His-tag
nitrilotriacetic acid (NTA)
protein binding
self-assembled monolayers (SAMs)
spectroscopic ellipsometry
Online Access:https://doi.org/10.3762/bjnano.7.48

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