Quality control and evaluation of Inonotus obliquus using HPLC method with novel marker compounds
Abstract Current quality control of Inonotus obliquus requires chromogen complex content limit of 10% in accordance with the State Pharmacopoeia of the Union of Soviet Socialist Republics. However, this causes ambiguous results, impeding precise quality control. To improve ambiguous quality control...
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doaj-201193243ebb4af8ac6f107467549b502020-12-06T12:03:21ZengSpringerOpenJournal of Analytical Science and Technology2093-33712020-12-011111810.1186/s40543-020-00249-zQuality control and evaluation of Inonotus obliquus using HPLC method with novel marker compoundsJin Hyeok Kim0Seol Kyu Park1Chong Woon Cho2Ba Vinh Le3Young Ho Kim4Haiying Bao5Kyung Tae Kim6Jong Seong Kang7College of Pharmacy, Chungnam National UniversityCollege of Pharmacy, Chungnam National UniversityCollege of Pharmacy, Chungnam National UniversityCollege of Pharmacy, Chungnam National UniversityCollege of Pharmacy, Chungnam National UniversityCollege of Chinese Medicinal Materials, Jilin Agricultural UniversityDepartment of Food Science and Technology, Dong-Eui UniversityCollege of Pharmacy, Chungnam National UniversityAbstract Current quality control of Inonotus obliquus requires chromogen complex content limit of 10% in accordance with the State Pharmacopoeia of the Union of Soviet Socialist Republics. However, this causes ambiguous results, impeding precise quality control. To improve ambiguous quality control criteria, this study developed a new HPLC method using two novel marker compounds (inotodiol and 3β-hydroxylanosta-8,24-dien-21-al) to control the quality control of I. obliquus. The HPLC analysis was carried out in a C18 column with an isocratic elution of 95% acetonitrile at 210 nm. The developed method validated in terms of linearity, precision, accuracy, and recovery. The content criteria were established by the linear regression method and relative standard deviation method. As the results of the quantitative monitoring, 1.165 mg/g of inotodiol and 1.717 mg/g of 3β-hydroxylanosta-8,24-dien-21-al, calculated by the relative standard deviation method, were suggested new quality criteria. A new HPLC method was developed to improve current quality control of I. obliquus and new lower content criteria were proposed as a result of quantitative monitoring. Graphical abstracthttps://doi.org/10.1186/s40543-020-00249-zInonotus obliquusInotodiol3β-Hydroxylanosta-8,24-dien-21-alHPLCQuality control |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jin Hyeok Kim Seol Kyu Park Chong Woon Cho Ba Vinh Le Young Ho Kim Haiying Bao Kyung Tae Kim Jong Seong Kang |
spellingShingle |
Jin Hyeok Kim Seol Kyu Park Chong Woon Cho Ba Vinh Le Young Ho Kim Haiying Bao Kyung Tae Kim Jong Seong Kang Quality control and evaluation of Inonotus obliquus using HPLC method with novel marker compounds Journal of Analytical Science and Technology Inonotus obliquus Inotodiol 3β-Hydroxylanosta-8,24-dien-21-al HPLC Quality control |
author_facet |
Jin Hyeok Kim Seol Kyu Park Chong Woon Cho Ba Vinh Le Young Ho Kim Haiying Bao Kyung Tae Kim Jong Seong Kang |
author_sort |
Jin Hyeok Kim |
title |
Quality control and evaluation of Inonotus obliquus using HPLC method with novel marker compounds |
title_short |
Quality control and evaluation of Inonotus obliquus using HPLC method with novel marker compounds |
title_full |
Quality control and evaluation of Inonotus obliquus using HPLC method with novel marker compounds |
title_fullStr |
Quality control and evaluation of Inonotus obliquus using HPLC method with novel marker compounds |
title_full_unstemmed |
Quality control and evaluation of Inonotus obliquus using HPLC method with novel marker compounds |
title_sort |
quality control and evaluation of inonotus obliquus using hplc method with novel marker compounds |
publisher |
SpringerOpen |
series |
Journal of Analytical Science and Technology |
issn |
2093-3371 |
publishDate |
2020-12-01 |
description |
Abstract Current quality control of Inonotus obliquus requires chromogen complex content limit of 10% in accordance with the State Pharmacopoeia of the Union of Soviet Socialist Republics. However, this causes ambiguous results, impeding precise quality control. To improve ambiguous quality control criteria, this study developed a new HPLC method using two novel marker compounds (inotodiol and 3β-hydroxylanosta-8,24-dien-21-al) to control the quality control of I. obliquus. The HPLC analysis was carried out in a C18 column with an isocratic elution of 95% acetonitrile at 210 nm. The developed method validated in terms of linearity, precision, accuracy, and recovery. The content criteria were established by the linear regression method and relative standard deviation method. As the results of the quantitative monitoring, 1.165 mg/g of inotodiol and 1.717 mg/g of 3β-hydroxylanosta-8,24-dien-21-al, calculated by the relative standard deviation method, were suggested new quality criteria. A new HPLC method was developed to improve current quality control of I. obliquus and new lower content criteria were proposed as a result of quantitative monitoring. Graphical abstract |
topic |
Inonotus obliquus Inotodiol 3β-Hydroxylanosta-8,24-dien-21-al HPLC Quality control |
url |
https://doi.org/10.1186/s40543-020-00249-z |
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